Hydrogel from Acellular Porcine Adipose Tissue Accelerates Wound Healing by Inducing Intradermal Adipocyte Regeneration

Tan, Qiuwen; Tang, Shenli; Zhang, Yi; Yang, Jiqiao; Wang, Zhu-Le; Xie, Huiqi; Lv, Qing

doi:10.1016/j.jid.2018.08.013

Cited by 22 publications

(16 citation statements)

References 29 publications

(38 reference statements)

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“…29 Furthermore, SVF can provide an enhanced biological framework for adipocytes, thereby favoring adipose tissue survival. 30 These observations indicated that the combination of adipocytes and SVF might be an important factor in improving the efficacy of rejuvenation therapy. Certainly, many researchers also reported a similar positive effect of platelet-rich plasma (PRP) addition as an alternative approach in fat grafting.…”

Section: Discussionmentioning

confidence: 95%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Background: Cell-assisted lipotransfer (CAL) promotes the survival of fat grafts with high vascular density and improves skin quality by increasing collagen content. However, no study has quantified the changes on the skin surface, and rigorous methodological evaluations are still lacking. Design: Fifty patients were recruited and randomly divided into two groups: an experimental group (n = 25) that underwent a stromal vascular fraction (SVF)-assisted fat graft and a control group (n = 25) that underwent fat graft only. Methods: The SVF cells were counted, tested in terms of viability, and characterized. The volumes of whole faces were determined by using a 3D scanner and Geomagic software preoperation, immediately after surgery, and 6 months postoperation. Facial skin qualities, including spots, wrinkles, texture, pores, UV spots, brown spots, red areas, and porphyrins, were detected by a VISIA skin detector preoperation and 6 months postoperation. A visual analog scale was used for clinical evaluation. Results: The cell pellet contained 1-3 × 10 7 /mL of fresh SVF cells. The cell viability exceeded 98%. The immunophenotyping characteristics and stemness were consistent with the features of adipose-derived stem cells (ADSCs). The survival rate of SVF-enriched fat grafts was significantly higher than that of control grafts: 77.6% ±11.6% versus 56.2% ±9.5% (p < 0.001). The VISIA values of wrinkles (19.3 ± 6.6 versus 10.9 ± 5.5, p < 0.001) and texture (15.8 ± 7.0 versus 10.3 ± 5.0, p < 0.01) were significantly higher in SVF-enriched group than in control group at 6 months postoperation. During long-term follow-up, the majority of patients in both groups were satisfied with the final facial esthetic results.

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Section: Discussionmentioning

confidence: 95%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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“…In addition, they can facilitate adhesion, survival, and differentiation of stem cells, as well as promoting host cell infiltration and revascularization ( Nie et al, 2015 ; Bankoti et al, 2020a ). Acellular matrices can be isolated from full skins ( Hsieh et al, 2020 ; Lin et al, 2020 ) and adipose tissues ( Dongen et al, 2019 ; Tan et al, 2019 ; Pu et al, 2020 ). In addition, acellular matrices are genetically tunable to adjust cell invasion through thrombospondin-2 knockout resulted in faster wound healing ( Morris et al, 2018 ).…”

Section: Fabrication Of Hydrogel For Wound Healingmentioning

confidence: 99%

Biomimetic Hydrogels to Promote Wound Healing

Fan

Saha

Hanjaya‐Putra

2021

Front. Bioeng. Biotechnol.

102

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Wound healing is a common physiological process which consists of a sequence of molecular and cellular events that occur following the onset of a tissue lesion in order to reconstitute barrier between body and external environment. The inherent properties of hydrogels allow the damaged tissue to heal by supporting a hydrated environment which has long been explored in wound management to aid in autolytic debridement. However, chronic non-healing wounds require added therapeutic features that can be achieved by incorporation of biomolecules and supporting cells to promote faster and better healing outcomes. In recent decades, numerous hydrogels have been developed and modified to match the time scale for distinct stages of wound healing. This review will discuss the effects of various types of hydrogels on wound pathophysiology, as well as the ideal characteristics of hydrogels for wound healing, crosslinking mechanism, fabrication techniques and design considerations of hydrogel engineering. Finally, several challenges related to adopting hydrogels to promote wound healing and future perspectives are discussed.

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“…Studies also showed that conditioned medium from cultured adipose-derived stem cells have the ability to promote tissue regeneration [21,22]. On the other hand, acellular adipose tissue as an important biomaterial, could also promote soft tissue regeneration [23,24].…”

Section: Introductionmentioning

confidence: 99%

Cell-free fat extract promotes tissue regeneration in a tissue expansion model

Deng

Wang

et al. 2020

Stem Cell Res Ther

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Background Tissue expansion techniques play an important role in plastic surgery. How to improve the quality of the expanded skin and shorten the expansion period are still worth investigating. Our previous studies found that a cell-free fat extract (CEFFE) possessed pro-angiogenic and pro-proliferative activities. However, the role of CEFFE on tissue expansion has remained unclear. The purpose of this study was to evaluate the effect of CEFFE on tissue expansion. Methods A rat tissue expansion model was used. Animals were treated with CEFFE by subcutaneous injection. After 4 weeks of tissue expansion, the skin necrosis and retraction rates were evaluated, the thicknesses of the epidermis and dermis were determined by histological analyses, blood vessel density was measured by anti-CD31 staining, cell proliferation was assessed by proliferating cell nuclear antigen staining, and the expression of specific proteins was evaluated by western blot analyses. In addition, the effects of CEFFE on the proliferation and cell cycle of cultured HaCaT cells were evaluated in vitro. Results CEFFE treatment significantly decreased the necrosis rate and retraction of the expanded skin. The thickness of the epidermal and dermal layers was higher in CEFFE-treated compared to untreated skin. The density of blood vessels and cell proliferation in the epidermis of the expanded skin was improved by CEFFE treatment. In addition, CEFFE treatment significantly increased the expression of the vascular endothelial growth factor receptor, epidermal growth factor receptor, collagen type 1, and collagen type 3. CEFFE also increased the proliferation of HaCaT cells in culture. Conclusions CEFFE improves the quality of the expanded skin by promoting angiogenesis and cell proliferation. It could be potentially used clinically for augmenting tissue expansion.

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Hydrogel from Acellular Porcine Adipose Tissue Accelerates Wound Healing by Inducing Intradermal Adipocyte Regeneration

Cited by 22 publications

References 29 publications

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Biomimetic Hydrogels to Promote Wound Healing

Cell-free fat extract promotes tissue regeneration in a tissue expansion model

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