Preparation and characterization of poly(ethylene oxide)/zinc oxide nanofibrous scaffold for chronic wound healing applications

Nosrati, Hamed; Khodaei, Mohammad; Dehkordi, Mehdi Banitalebi; Alizadeh, Morteza; Asadpour, Shiva; Sharifi, Esmaeel; Ai, Jafar; Soleimannejad, Mostafa

doi:10.17219/pim/128378

Cited by 19 publications

(14 citation statements)

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“…Each experiment was performed three times at the specified culture durations of 1, 3, and 7 days in a 96‐well plate (3 × 10 3 cells/well) as reported previously. 21 , 22 Finally, the microplate reader (ChroMate‐4300, FL, USA) at 570 nm was used to measure the absorbance of the samples. For better assessment and evaluating biocompatibility, after assessing the cytotoxicity with MTT assay, we evaluated the viability as well as cell proliferation using cell counting kit‐8 (CCK‐8) using fibroblast (L929) cells, respectively.…”

Section: Methodsmentioning

confidence: 99%

“… 14 , 20 The simplest and most effective way to enhance the electrospinning capabilities of chitosan is blending with another polymer (e.g., polyethylene oxide) with high electrospinning potential. 21 , 22 Polyethylene oxide (PEO) is a biocompatible, noncytotoxic, and hydrophilic polymer and is widely used in biomedical applications. Moreover, PEO is employed in combination with chitosan to form uniform nanofibers with suitable mechanical properties for wound healing and tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

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Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect

Bagheri

Validi

Gholipour

et al. 2021

Bioengineering & Transla Med

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118

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Bacterial wound infection is one of the most common nosocomial infections.The unnecessary employment of antibiotics led to raising the growth of antibioticresistant bacteria. Accordingly, alternative armaments capable of accelerating wound healing along with bactericidal effects are urgently needed. Considering this, we fabricated chitosan (CS)/polyethylene oxide (PEO) nanofibers armed with antibacterial silver and zinc oxide nanoparticles. The nanocomposites exhibited a high antioxidant effect and antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Besides, based on the results of the cell viability assays, the optimum concentration of ZnONPs and AgNPs in the nanofibrous mats is 0.2% w/v and 0.08% w/v respectively and had no cytotoxicity on fibroblast cells. The scaffold also showed good blood compatibility according to the effects of coagulation time. As well as significant fibroblast migration and proliferation on the wound margin, according to wound-healing assay. All in all, the developed biocompatible, antioxidant, and antibacterial Ag-ZnO NPs incorporated CS/PEO nanofibrous mats showed their potential as an effective wound dressing.antibacterial, antioxidant, chitosan/polyethylene oxide nanofibers, electrospun, silver nanoparticles, wound healing, zinc oxide nanoparticles | INTRODUCTIONWound infection is a common and unsolved issue in preventing wound healing. Any kind of wound on the skin is susceptible to the accumulation of infectious bacteria. 1 With the spread of antibiotic-resistant bacteria, employing antibiotics to prevent and treat wound infections is inefficient.In addition, it imposes extra costs on the patient and society. Consequently, alternative materials are pivotal to combat pathogens. 2,3

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect

Bagheri

Validi

Gholipour

et al. 2021

Bioengineering & Transla Med

Self Cite

118

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“…FTIR spectra of ZnO nanoparticles (Figure 3) showed an intense peak at 400-500 cm À1 (Zn-O) and a broad peak at 3331-3441 cm À1 (O-H) [26,27].…”

Section: Ftir Datamentioning

confidence: 99%

“…FTIR spectra of the formulated chemo drugs were divided into three major fractions in terms of the compositions of drugs: ZnO structure (400-500 cm À1 ) [26,27], CP structure (1379 cm À1 ) and DOX structure (1730 cm À1 ). The FTIR spectra of CP-DOX-ZnO complexes were slightly shifted because of effect of ester linkage between chemo drugs and ZnO nanoparticles.…”

Section: Surface Characterizationmentioning

confidence: 99%

Antitumor activities of carboplatin–doxorubicin–ZnO complexes in different human cancer cell lines (breast, cervix uteri, colon, liver and oral) under UV exposition

Pairoj

Damrongsak

et al. 2021

Artificial Cells, Nanomedicine, and Biotechnology

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This study aimed to examine the pharmacological profiles of multiple chemo drug candidates in systematic circulation to enhance their specific interactions with five human cancer cell lines. ZnO nanoparticles were successfully bound with chemo drugs via physical adsorption. The drug loading capacity was confirmed by FTIR, whereas the loading efficiency was determined via UV-vis spectrometry. The mean hydrodynamic size increased to 69-82 nm after chemo-drug immobilization via non-covalent interaction with ZnO. Among the nine formulated chemo drugs, the carboplatin (CP)-doxorubicin (DOX)-ZnO complex under UV light irradiation exhibited high sensitivity towards human breast adenocarcinoma cells without affecting human keratinocyte immortal cells with an IC 50 of 0.137 mg/mL, whereas the loading capacity and efficiency of CP-DOX-ZnO were 77.81% and 99.05%, respectively. Fluorescence images confirmed that CP-DOX-ZnO using DOX served as a fluorescence enhancer specifically bound onto the cell membranes, which became almost saturated after 24 h incubation. Carboplatin-DOX-ZnO was possibly endocytosed by cancer cells and was selectively internalized into the target cells; thus, free chemo drug was released in the cytoplasm, which induced acute apoptosis. This resulted in complete inhabitation of growth signal of target cancer cells.

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“…Kiran Kumar et al [ 142 ] studied zinc oxide nanoparticles synthesized from a Raphanus sativus root extract and showed their antimicrobial property against Escherichia fergusonii and E. coli strains that can be further explored for wound-healing potential. Apart from these, many reports have also indicated that the application of zinc oxide nanoparticles/or their biocomposites, irrespective of their application, possessed potential wound-healing ability and help in wound healing [ 143 , 144 , 145 , 146 ].…”

Section: Biomedical Applications Of Plant-mediated Zinc Oxide Nanoparticlesmentioning

confidence: 99%

Plant-Mediated Zinc Oxide Nanoparticles: Advances in the New Millennium towards Understanding Their Therapeutic Role in Biomedical Applications

et al. 2021

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Zinc oxide nanoparticles have become one of the most popular metal oxide nanoparticles and recently emerged as a promising potential candidate in the fields of optical, electrical, food packaging, and biomedical applications due to their biocompatibility, low toxicity, and low cost. They have a role in cell apoptosis, as they trigger excessive reactive oxygen species (ROS) formation and release zinc ions (Zn2+) that induce cell death. The zinc oxide nanoparticles synthesized using the plant extracts appear to be simple, safer, sustainable, and more environmentally friendly compared to the physical and chemical routes. These biosynthesized nanoparticles possess strong biological activities and are in use for various biological applications in several industries. Initially, the present review discusses the synthesis and recent advances of zinc oxide nanoparticles from plant sources (such as leaves, stems, bark, roots, rhizomes, fruits, flowers, and seeds) and their biomedical applications (such as antimicrobial, antioxidant, antidiabetic, anticancer, anti-inflammatory, photocatalytic, wound healing, and drug delivery), followed by their mechanisms of action involved in detail. This review also covers the drug delivery application of plant-mediated zinc oxide nanoparticles, focusing on the drug-loading mechanism, stimuli-responsive controlled release, and therapeutic effect. Finally, the future direction of these synthesized zinc oxide nanoparticles’ research and applications are discussed.

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Preparation and characterization of poly(ethylene oxide)/zinc oxide nanofibrous scaffold for chronic wound healing applications

Cited by 19 publications

References 0 publications

Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect

Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect

Antitumor activities of carboplatin–doxorubicin–ZnO complexes in different human cancer cell lines (breast, cervix uteri, colon, liver and oral) under UV exposition

Plant-Mediated Zinc Oxide Nanoparticles: Advances in the New Millennium towards Understanding Their Therapeutic Role in Biomedical Applications

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