Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect

Abstract

Bacterial wound infection is one of the most common nosocomial infections.The unnecessary employment of antibiotics led to raising the growth of antibioticresistant bacteria. Accordingly, alternative armaments capable of accelerating wound healing along with bactericidal effects are urgently needed. Considering this, we fabricated chitosan (CS)/polyethylene oxide (PEO) nanofibers armed with antibacterial silver and zinc oxide nanoparticles. The nanocomposites exhibited a high antioxidant effect and antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Besides, based on the results of the cell viability assays, the optimum concentration of ZnONPs and AgNPs in the nanofibrous mats is 0.2% w/v and 0.08% w/v respectively and had no cytotoxicity on fibroblast cells. The scaffold also showed good blood compatibility according to the effects of coagulation time. As well as significant fibroblast migration and proliferation on the wound margin, according to wound-healing assay. All in all, the developed biocompatible, antioxidant, and antibacterial Ag-ZnO NPs incorporated CS/PEO nanofibrous mats showed their potential as an effective wound dressing.antibacterial, antioxidant, chitosan/polyethylene oxide nanofibers, electrospun, silver nanoparticles, wound healing, zinc oxide nanoparticles | INTRODUCTIONWound infection is a common and unsolved issue in preventing wound healing. Any kind of wound on the skin is susceptible to the accumulation of infectious bacteria. 1 With the spread of antibiotic-resistant bacteria, employing antibiotics to prevent and treat wound infections is inefficient.In addition, it imposes extra costs on the patient and society. Consequently, alternative materials are pivotal to combat pathogens. 2,3

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