Antitumor activities of carboplatin–doxorubicin–ZnO complexes in different human cancer cell lines (breast, cervix uteri, colon, liver and oral) under UV exposition

Abstract

This study aimed to examine the pharmacological profiles of multiple chemo drug candidates in systematic circulation to enhance their specific interactions with five human cancer cell lines. ZnO nanoparticles were successfully bound with chemo drugs via physical adsorption. The drug loading capacity was confirmed by FTIR, whereas the loading efficiency was determined via UV-vis spectrometry. The mean hydrodynamic size increased to 69-82 nm after chemo-drug immobilization via non-covalent interaction with ZnO. Among the nine formulated chemo drugs, the carboplatin (CP)-doxorubicin (DOX)-ZnO complex under UV light irradiation exhibited high sensitivity towards human breast adenocarcinoma cells without affecting human keratinocyte immortal cells with an IC 50 of 0.137 mg/mL, whereas the loading capacity and efficiency of CP-DOX-ZnO were 77.81% and 99.05%, respectively. Fluorescence images confirmed that CP-DOX-ZnO using DOX served as a fluorescence enhancer specifically bound onto the cell membranes, which became almost saturated after 24 h incubation. Carboplatin-DOX-ZnO was possibly endocytosed by cancer cells and was selectively internalized into the target cells; thus, free chemo drug was released in the cytoplasm, which induced acute apoptosis. This resulted in complete inhabitation of growth signal of target cancer cells.

Innovative microwave-assisted biosynthesis of copper oxide nanoparticles loaded with platinum(ii) based complex for halting colon cancer: cellular, molecular, and computational investigations

“…A Similar approach was performed previously where Cr together with doxorubicin (Dox) were dual loaded onto ZnO nanoparticles and proved highly efficient at increasing the sensitivity and selectivity of chemo drugs to human breast adenocarcinoma cells. 70 All the above reinforces the idea that our newly synthesized Cr@CuO NPs possess much better therapeutic potential than free Cr or CuO Nps in the treatment of colon cancer.…”

“…A Similar approach was performed previously where Cr together with doxorubicin (Dox) were dual loaded onto ZnO nanoparticles and proved highly efficient at increasing the sensitivity and selectivity of chemo drugs to human breast adenocarcinoma cells. 70 …”

Nanoparticles Loaded with Platinum Drugs for Colorectal Cancer Therapy

“…These NPs displayed a loading capacity and efficiency of 77.81% and 99.05%, respectively. The anticancer studies of ZnO NPs co-incorporated with carboplatin and doxorubicin showed good antitumor efficacy against HT-29 cancer cell lines under the influence of UV with an IC 50 of 0.173 µg/mol and they were selective in their uptake into the target cells where the loaded drugs were released to induce acute apoptosis with a significant absence of a growth signal of target cancer cells [ 93 ].…”