Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering

Kim, Hye Lee; Jung, Gil Yong; Yoon, Jun Ho; Han, Jung Suk; Park, Yoon Jeong; D, Kim; Zhang, Miqin; Kim, Dae Joon

doi:10.1016/j.msec.2015.04.033

Cited by 211 publications

(114 citation statements)

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“…Alginate, a natural resource of polysaccharides derived from seaweed, is widely used in biomedical purposes [17,18]. Recently, alginate-based scaffolds, such as HA/alginate and HA/alginate/chitosan composites, have been developed for bone tissue engineering [19,20]. Particularly, HA/ alginate composite scaffolds have been found to increase the pore size of bone [21,22].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Porous Hydroxyapatite and Alginate Composite Scaffolds for Bone Tissue Engineering

2018

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Objective: To prepare and characterize composite scaffolds of a hydroxyapatite (HA) and an alginate having high viscosity. Materials and Methods:HA powder was synthesized using wet chemical precipitation, the alginate powder was extracted from the Sargassum duplicatum seaweed, and the HA/alginate composite scaffolds were prepared by freeze-drying. X-ray diffraction and Fourier transform infrared techniques were utilized to characterize the HA and alginate, and electron microscopy was used to evaluate the HA and the HA/alginate composite scaffolds. The HA/alginate composite scaffold obtained from the commercially available HA and alginate powders were employed as a comparison.Results: Synthesized HAs were identified as the HA phase, which contained absorbed water, phosphate, and carbonate groups. The extracted alginate contained the carboxyl, cyclic ether and hydroxyl groups. The scaffolds prepared from the HA and alginate mixture were three-dimensional and containing interconnected pores with a diameter ranging from 150 to 300 µm and pore walls of a composite construction. Conclusion:A three-dimensional scaffold was produced using a freeze-drying method from a composite of HA and the high viscosity alginate solution. The scaffold was highly porous and showed interconnected pores, with a diameter ranging from 150 to 300 µm.

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Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Porous Hydroxyapatite and Alginate Composite Scaffolds for Bone Tissue Engineering

2018

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“…The compressive strength was determined by crushing the cubic-shaped scaffolds with a dimension of 10 10 10 mm until it reached 30 % deformation using a computer-controlled texture analyzer (CT3, Brookfield, USA) with a ramp rate of 0.5 mm s -1 [22,23]. Three identical specimens for each sample group were used in the compressive testing and average results were taken.…”

Section: Characterization Of Bone Scaffoldsmentioning

confidence: 99%

Fabrication of Bone Scaffolds from Cockle Shell Waste

2019

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Bone scaffold is a three-dimensional structure composed of materials that could enhance bone regeneration. Bone scaffolds were prepared using freeze-drying by varying the cockle shell powder concentration where sodium alginate acted as matrix. The scaffolds were then characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, texture analyzer, and liquid displacement method. The bioactivity of the scaffolds was evaluated by immersion into a simulated body fluid solution. Cockle shell powder concentrations affected the bone scaffold characteristics. The increment of the powder concentrations improved the physicochemical properties and bioactivity of the scaffolds.

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“…23 Estudos mais recentes mostram uma preocupação em aprimorar as propriedades mecânicas e biológicas da quitosana. 6,11,13,14,23,[28][29][30][31][32][33][34][35][36][37][38] Sua associação com outros polímeros tem sido largamente estudada, e essa é a tendência de perspectivas futuras no que se refere à pesquisa com quitosana. Essa complexa integração entre engenharia e biologiaáreas tão distintas, mas, ao mesmo tempo, tão intrinsecamente interligadas -proporciona uma verdadeira relação…”

Section: A Quitosana Para O Reparo óSseounclassified

Chitosan Scaffolds - Physico -Chemical and Biological Properties for Bone Repair

Rolim¹,

Carvalho²,

Costa³

et al. 2018

Rev. Virtual Quim.

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Abstract:Chitosan is a versatile polymer, whose applicability in bone repair has been widely investigated. The main biological properties of this polymer are related to its biocompatibility, slow degradation, and controlled bioreabsorption. It also presents bioactivity in the induction or stimulation of osteoblasts, and antimicrobial, antifungal, hemostatic and angiogenic properties. Due to its characteristics, chitosan may integrate the structure of anionic polyelectrolytes, such as proteins and polymers which makes possible the obtainment of different biomaterials with properties, applications and distinct forms of presentation possible. Chitosan, when used in a three-dimensional (3D) matrix form, acts as a physical scaffold for tissue regeneration, through migration, adhesion and proliferation of bone cells. Its polymeric structure and its physical and chemical attributes allow the improvement of its bioactive properties through the addition of composites, cells, or growth factors. Thus, to the extent that there is improvement in the microstructure molecular of the chitosan, there are modifications in its properties as a reference biomaterial in bone tissue bioengineering. Among the modifiable characteristics of these biomaterials in the base of the chitosan, the mechanical strength, surface topography, hydrophilicity, elasticity, porosity, interconnectivity, biodegradation, molar mass, and its pH are to be noted. This paper, therefore, aims to address the main aspects (micro and macro-structural) related to the structure of chitosan in bone tissue repair, as well as its impact on the biological behavior of 3D arrays when it's used alone or in combination with other biomaterials in regenerative medicine.Keywords: Chitosan; biomaterial; three-dimensional matrix; bone repair. ResumoA quitosana é um polímero versátil, cuja aplicabilidade no reparo ósseo tem sido amplamente investigada. As principais propriedades biológicas desse polímero estão relacionadas à sua biocompatibilidade, à degradação lenta e à biorreabsorção controlada. Apresenta também bioatividade na indução ou estimulação de osteoblastos, ação antimicrobiana, antifúngica, hemostática e angiogênica. Em razão de suas características, podem integrar a sua estrutura os polieletrólitos aniônicos, os polímeros e proteínas, o que possibilita a obtenção de diferentes biomateriais com propriedades, aplicações e formas de apresentação distintas. Quando utilizada sob a forma de matriz tridimensional (3D), atua como arcabouço físico para a regeneração tecidual, pela migração, adesão e proliferação das células ósseas. Sua estrutura polimérica e seus atributos físico-químicos permitem o aprimoramento de suas propriedades bioativas por meio da adição de compósitos, células ou fatores de crescimento. E assim, na medida em que há aprimoramento na microestrutura molecular da quitosana, há modificações nas suas propriedades como biomaterial de referência na bioengenharia tecidual óssea. Dentre as características modificáveis desses biomateriais à base de quitosana, d...

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Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering

Cited by 211 publications

References 27 publications

Preparation and Characterization of Porous Hydroxyapatite and Alginate Composite Scaffolds for Bone Tissue Engineering

Preparation and Characterization of Porous Hydroxyapatite and Alginate Composite Scaffolds for Bone Tissue Engineering

Fabrication of Bone Scaffolds from Cockle Shell Waste

Chitosan Scaffolds - Physico -Chemical and Biological Properties for Bone Repair

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