Preparation and characterization of nano-hydroxyapatite within chitosan matrix

Rogina, Anamarija; Ivanković, Marica; Ivanković, Hrvoje

doi:10.1016/j.msec.2013.07.008

Cited by 54 publications

(30 citation statements)

References 19 publications

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“…1). 1,26 Results of our previous study 22 have indicated possible interactions between in situ formed HA and chitosan. In FTIR spectra of CS/HA composites the phosphate band shifting was observed, regarding the phosphate band of pure hydroxyapatite prepared at the same conditions.…”

Section: Scaffold's Characterizationmentioning

confidence: 95%

In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

et al. 2015

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Highly porous chitosan/hydroxyapatite composite structures with different weight ratios (100/0; 90/10; 80/20; 70/30; 60/40; 50/50; 40/60) have been prepared by precipitation method and freeze-gelation technique using calcite, urea phosphate and chitosan as starting materials. The composition of prepared composite scaffolds was characterized by X-ray diffraction analysis and Fourier transformed infrared spectroscopy, while morphology of scaffolds was imaged by scanning electron microscopy. Mercury intrusion porosimetry measurements of prepared scaffolds have shown different porosity and microstructure regarding to the HA content, along with SEM observations of scaffolds after being immersed in physiological medium. The results of swelling capacity and compressive strength measured in Dulbecco's phosphate buffer saline (DPBS) have shown higher values for composite scaffolds with lower in situ HA content. Viability, proliferation and differentiation of MC3T3-E1 cells seeded on different scaffolds have been evaluated by live dead assay and confocal scan microscopy. Our results suggest that the increase of HA content enhance osteoblast differentiation confirming osteogenic properties of highly porous CS/HA scaffolds for tissue engineering applications in bone repair.

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Section: Scaffold's Characterizationmentioning

confidence: 95%

In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

et al. 2015

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“…Generally, there is several ways to produce such composite materials either by mixing the solid inorganic powder with organic polymer or by preparing in situ preparing the inorganic materials from their ionic precursors in the polymer. The most used method is the in situ co-precipitation synthesis [10].…”

Section: Mai K Hassan Et Almentioning

confidence: 99%

The Effect of Different Solvents for Chitosan Solubilization on The Crystal Growth of in situ Prepared Hydroxyapatite

2017

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C HITOSAN/hydroxyapatite (CS/HA) nanocomposites have been widely fabricated for potential use in bone defects due to their high biocompatibility and similarity to the natural bone. In the current study, HA nanocrystals were prepared by co-precipitation method in CS which was initially dissolved in different concentrations of acrylic acid and acetic acid (2 and 4 %). The effect of chitosan solvents on the morphology of HA nanocrystals, the crystallite sizes, and the physical binding with CS were investigated by different techniques including Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and transmission electron microscope (TEM). The FT-IR and XPS results showed that the interaction between CS and HA in the nanocomposites is predominantly dependent on the type of solvent used to dissolve CS. When acetic acid was used, the interaction between CS and HA was stronger when compared to acrylic acid. Quantitative XPS results showed that the atomic percentage of nitrogen (N1s) of the amine groups of CS was less in the case of the composites prepared after dissolving CS in acrylic acid compared to acetic acid. The amine groups of CS (−NH 2 ) can start the nucleation of HA crystals and remain bounded to the surface of the grown crystal through chelation with calcium ions on the crystal facets. In both organic solvents, HA crystals were found to grow along their c-axis direction into rodshape but the growth in the width was more restricted in case of acetic acid. The results in the current study indicated that the type of CS solvents is determining the strength of the physical interaction between the CS and HA. This might be a crucial factor to control and enhance the mechanical and the biological behaviors of the nanocomposites.

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“…It was added to a chitosan/gelatin mixture to prepare a membrane which may potentially be useful as a biomaterial for engineering bone tissue . It should be noted that the in situ precipitation method may lead to nanocomposites that show some features of natural bone both in composition and in microstructure . It has been shown that biomaterials such as chitosan and calcium phosphate composites can show excellent properties such as biocompatibility, biofunctionality and non‐antigenic properties too .…”

Section: Chitosan Nanocompositesmentioning

confidence: 99%

Biopolymeric nanocomposites for potential biomedical applications

Sionkowska

2016

Polymer International

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This review reports recent advances in the field of biopolymeric composites and nanocomposites for potential biomedical applications. These materials have attracted both academic and, for several composites, industrial attention because they exhibit properties required in the biomedical field. Herein, the structure, preparation and properties of biopolymeric composite blends are discussed in general, and detailed examples are also drawn from the scientific literature and practical work. In this review the most common natural polymers collagen, chitosan and their composites and nanocomposites with inorganic particles are discussed.

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Preparation and characterization of nano-hydroxyapatite within chitosan matrix

Cited by 54 publications

References 19 publications

In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

The Effect of Different Solvents for Chitosan Solubilization on The Crystal Growth of in situ Prepared Hydroxyapatite

Biopolymeric nanocomposites for potential biomedical applications

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