In Situ Hydroxyapatite Content Affects the Cell Differentiation on Porous Chitosan/Hydroxyapatite Scaffolds

Abstract

Highly porous chitosan/hydroxyapatite composite structures with different weight ratios (100/0; 90/10; 80/20; 70/30; 60/40; 50/50; 40/60) have been prepared by precipitation method and freeze-gelation technique using calcite, urea phosphate and chitosan as starting materials. The composition of prepared composite scaffolds was characterized by X-ray diffraction analysis and Fourier transformed infrared spectroscopy, while morphology of scaffolds was imaged by scanning electron microscopy. Mercury intrusion porosimetry measurements of prepared scaffolds have shown different porosity and microstructure regarding to the HA content, along with SEM observations of scaffolds after being immersed in physiological medium. The results of swelling capacity and compressive strength measured in Dulbecco's phosphate buffer saline (DPBS) have shown higher values for composite scaffolds with lower in situ HA content. Viability, proliferation and differentiation of MC3T3-E1 cells seeded on different scaffolds have been evaluated by live dead assay and confocal scan microscopy. Our results suggest that the increase of HA content enhance osteoblast differentiation confirming osteogenic properties of highly porous CS/HA scaffolds for tissue engineering applications in bone repair.

Porous Chitosan/Nano-Hydroxyapatite Composite Scaffolds Incorporating Simvastatin-Loaded PLGA Microspheres for Bone Repair

“…3a, b). The mean size of HA-0.5, HA-1, and HA-2 scaffolds was >100 μm, which was within the recommended pore size range for bone regeneration [Rogina et al, 2016]. The addition of HA induced a concentration-dependent increase in the compressive modulus of the scaffolds (Fig.…”

Macroporous poly(lactic acid) construct supporting the osteoinductive porous chitosan-based hydrogel for bone tissue engineering

“…Our previous studies [18,19] have indicated highly porous chitosan-based scaffolds with 30% weight ratio of in situ formed hydroxyapatite as a suitable environment for differentiation of MC3T3-E1 preosteoblasts during 14 days of culture. In situ synthesis has provided good dispersion of formed HA particles and formation of nonstoichiometric more bioresorbable hydroxyapatite.…”

Estudo de hidroxiapatita contendo própolis de origem brasileira: caracterização, atividade antimicrobiana e efeito citotóxico dos materiais

“…As análises de FTIR foram realizadas com o objetivo de identificar os grupos funcionais específicos tanto para os extratos secos de própolis verde e vermelha como para as amostras (HA-ETOH80, HA-GP20, HA-GP8, HA-RP20 e HA-RP8). Para os extratos secos de própolis verde e vermelha, os espectros de FTIR foram obtidos com objetivo principal de identificar grupos funcionais característicos de fenólicos e flavonoides totais, principais responsáveis pela atividade antimicrobiana destes extratos, uma vez que a composição da própolis é bastante complexa e variável, apresentando mais de 300 substâncias (SFORCIN, 2016 adsorvido da atmosfera durante o processo de síntese, uma vez que o procedimento é realizado em sistema aberto (GUERRA-LOPEZ et al, 2015;HE;DONG;DENG, 2016;JAFARI et al, 2016;ROGINA et al, 2016;SINGH, 2016;ZHANG et al, 2016). A Tabela 8 mostra os sinais obtidos para cada banda de absorção para a amostra HA-ETOH80 e suas respectivas atribuições.…”

“…Nos espectros obtidos para as amostras HA-GP 20 e HA-GP8 ( Guerra-Lopez et al (2015); Rogina et al (2016); Srilakshmi, Saraf (2016); Zhang et al (2016) Fonte: Própria autoria.…”