Preparation and characterization of highly stable lipid nanoparticles with amorphous core of tuneable viscosity

Delmas, Thomas; Couffin, Anne-Claude; Bayle, Pierre Alain; Crecy, F. de; Neumann, Emmanuelle; Vinet, F.; Bardet, Michel; Bibette, Jérôme; Texier, Isabelle

doi:10.1016/j.jcis.2011.04.080

Cited by 70 publications

(85 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22,25 As previously described, 22 the heterogeneity of the lipids and surfactants mixtures constituting the nanodroplet favors its colloidal stability. In this study, typical results are presented using 50 nm diameter lipidots, but other nanoparticle sizes can easily be achieved modulating the surfactant to lipid ratio.…”

Section: Design and Colloïdal Properties Of Dye-loaded Lipid Nanopartmentioning

confidence: 89%

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging

et al. 2011

Self Cite

View full text Add to dashboard Cite

The use of fluorescent nanostructures can bring several benefits on the signal to background ratio for in vitro microscopy, in vivo small animal imaging, and image-guided surgery. Fluorescent quantum dots (QDs) display outstanding optical properties, with high brightness and low photobleaching rate. However, because of their toxic element core composition and their potential long term retention in reticulo-endothelial organs such as liver, their in vivo human applications seem compromised. The development of new dye-loaded (DiO, DiI, DiD, DiR, and Indocyanine Green (ICG)) lipid nanoparticles for fluorescence imaging (lipidots) is described here. Lipidot optical properties quantitatively compete with those of commercial QDs (QTracker(®)705). Multichannel in vivo imaging of lymph nodes in mice is demonstrated for doses as low as 2 pmols of particles. Along with their optical properties, fluorescent lipidots display very low cytotoxicity (IC(50) > 75 nM), which make them suitable tools for in vitro, and especially in vivo, fluorescence imaging applications.

show abstract

Section: Design and Colloïdal Properties Of Dye-loaded Lipid Nanopartmentioning

confidence: 89%

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Where W initial drug is the amount of drug added in the formulation and W free drug is the amount of drug in supernatant [26].…”

Section: Entrapment Efficiencymentioning

confidence: 99%

The design of naproxen solid lipid nanoparticles to target skin layers

Âkbari

Saeedi

Morteza‐Semnani

et al. 2016

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

(2016) The design of naproxen solid lipid nanoparticles to target skin layers. Colloids and Surfaces B: Biointerfaces, This version is available from Sussex Research Online: http://sro.sussex.ac.uk/67745/ This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published version. Copyright and reuse:Sussex Research Online is a digital repository of the research output of the University.Copyright and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable, the material made available in SRO has been checked for eligibility before being made available.Copies of full text items generally can be reproduced, displayed or performed and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Graphical Abstract Highlights  Naproxen solid lipid nanoparticles (Nap-SLN) can successfully be prepared by ultrasonication  Properties of these nanoparticles can be optimized by lipid concentrations  HLB of the surfactants played a crucial effect on properties of solid lipid nanoparticles  Nap-SLN increased the drug concentration in skin layer rather than systemic absorption Accepted Manuscript AbstractThe aim of the current investigation was to produce naproxen solid lipid nanoparticles (Nap-SLNs) by the ultrasonication method to improve its skin permeation and also to investigate the influence of Hydrophilic-lipophilic balance (HLB) changes on nanoparticles properties. The properties of obtained SLNs loaded with naproxen were characterized by photon correlation spectroscopy (PCS), transmission electron microscopy (TEM) and differential scanning calorimetry (DSC). FT-IR was also used to investigate any interaction between naproxen and the excipients used at the molecular level during the preparation of the SLNs. The performance of the formulations was investigated in terms of skin permeation and also the retention of the drug by the skin. It was found that generally, with increasing the lipid concentration, the average particle size and polydispersity index (PDI) ...

show abstract

“…Triply labeled lipidots containing the 3 tracers 3 H-CHE, 14 C-CHO, and DiD were obtained as 55 6 2 nm (mean 6 SD, n 5 3) droplets with a polydispersity of 0.19, in a concentrated particle dispersion (1.2 · 10 15 particles/mL after dialysis) with long-term stability (.120 wk at 4°C) (4,5). Because of the neutral PEG coating of the particles, the z potential of the droplets was close to 25 mV.…”

Section: Nanoparticle Characterizationmentioning

confidence: 99%

“…The low cytotoxicity and high loading capacity of synthetic lipidbased nanoparticles limit adverse side effects, enhance delivery to biologic targets, and reduce metabolism and distribution to undesired sites (1-3). Lipidots are newly described lipid nanoparticles based on oil-in-water nanoemulsion templates made of components generally regarded as safe (4,5), forming colloidal dispersions of lipid droplets with finely tuned core/shell properties and stable for several years (4,5). These particles display no cellular toxicity and can incorporate high amounts of organic fluorescent dyes (6).…”

mentioning

confidence: 99%

Synthetic Lipid Nanoparticles Targeting Steroid Organs

et al. 2013

Self Cite

View full text Add to dashboard Cite

Lipidots are original nanoparticulate lipid delivery vectors for drugs and contrast agents made from materials generally regarded as safe. Here, we characterized the in vivo stability, biodistribution, and pharmacokinetics of lipidots. Methods: Lipidots 55 nm in diameter and coated with a phospholipid/poly(ethyleneglycol) surfactant shell were triply labeled with 3 H-cholesteryl-hexadecyl-ether, cholesteryl-14 C-oleate, and the 1,19-dioctadecyl-3,3,39,39-tetramethylindotricarbocyanine infrared fluorescent dye and injected intravenously into immunocompetent Friend virus B-type mice. The pharmacokinetics and biodistribution of lipidots were analyzed quantitatively in serial samples of blood and tissue and with in vivo optical imaging and were refined by microscopic examination of selected target tissues. Results: The plasmatic half-life of lipidots was approximately 30 min. Radioactive and fluorescent tracers displayed a similar nanoparticle-driven biodistribution, indicative of the lipidots' integrity during the first hours after injection. Lipidots distributed in the liver and, surprisingly, in the steroid-rich organs adrenals and ovaries, but not in the spleen. This tropism was confirmed at the microscopic level by histologic detection of 1,19-dioctadecyl-3,3,39,39-tetramethylindotricarbocyanine. Nanoparticle loading with cholesterol derivatives increased accumulation in ovaries in a dosedependent manner. Conclusion: This previously unreported distribution pattern is specific to lipidots and attributed to their nanometric size and composition, conferring on them a lipoproteinlike behavior. The affinity of lipidots for steroid hormone-rich areas is of interest to address drugs and contrast agents to lipoprotein-receptor-overexpressing cancer cells found in hormone-dependent tumors.

show abstract

Preparation and characterization of highly stable lipid nanoparticles with amorphous core of tuneable viscosity

Cited by 70 publications

References 36 publications

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging

The design of naproxen solid lipid nanoparticles to target skin layers

Synthetic Lipid Nanoparticles Targeting Steroid Organs

Contact Info

Product

Resources

About