Preparation and characterization of a molecularly imprinted monolithic column for pressure‐assisted CEC separation of nitroimidazole drugs

Abstract

A polymethacrylate-based molecularly imprinted monolithic column bearing mixed functional monomers, using non-covalent imprinting approach, was designed for the rapid separation of nitroimidazole compounds. The new monolithic column has been prepared via simple in situ polymerization of 2-hydroxyethyl methacrylate, dimethylaminoethyl methacrylate and ethylene dimethacrylate, using (S)-ornidazole ((S)-ONZ) as template in a binary porogenic mixture consisting of toluene and dodecanol. The composition of the polymerization mixture was systematically altered and optimized by altering the amount of monomers as well as the composition of the porogenic solvent. The column performance was evaluated in pressure-assisted CEC mode. Separation conditions such as pH, voltage, amount of organic modifier and salt concentration were studied. The optimized monolithic column resulted in excellent separation of a group of structurally related nitroimidazole drugs within 10 min in isocratic elution condition. Column efficiencies of 99 000, 80 000, 103 000, 60 000 and 99 000 plates/m were obtained for metronidazole, secnidazole, ronidazole, tinidazole and dimetridazole, respectively. Parallel experiments were carried out using molecularly imprinted and non-imprinted capillary columns. The separation might be the result of combined effects including hydrophobic, hydrogen bonding and the imprinting cavities on the (S)-ONZ-imprinted monolithic column.

Determination of 5-nitroimidazole residues in milk by capillary electrochromatography with packed C18 silica beds

“…At the best of our knowledge, there is just one CEC method published for the determination of 5-NDZs [48]. Nevertheless, this work is focused on monolithic MIP-based capillary development and 5-NDZ standard separation is proposed without any application to real matrixes.…”

Recent developments and applications of molecularly imprinted monolithic column for HPLC and CEC

“…A new ( S )‐ONZ MIP monolithic column using 2‐hydroxyethyl methacrylate (HEMA) and dimethylaminoethyl methacrylate (DMAEMA) as the functional monomers and EDMA as cross‐linker was prepared and successfully used for the separation of nitroimidazole drugs 137. The performance of MIP monolith was evaluated in pressure‐assisted CEC, which involves the application of pressure in the CEC by using an HPLC pump 138, 139.…”

Liquid crystal-based molecularly imprinted nanoparticles with low crosslinking for capillary electrochromatography

“…Up to now, there are mainly three formats of MIP that can be applied to CEC, namely the monolith [13][14][15][16][17][18][19], the coating [20][21][22][23][24] and particle-based technique [25][26][27][28][29][30][31][32][33]. To extend the application of liquid crystal MIP, liquid crystalline MIP nanoparticles were tried for enantiomers separation in CEC mode.…”