Preparation and Characteristic of Hydroxyapatite Synthesized from Cuttlefish Bone by Precipitation Method

Abstract

Calcium phosphates ceramics such as a hydroxyapatite have composition and structure similar to be mineral component of bone. Hydroxyapatite could be production from natural and chemical source with various techniques. In this study, Calcium phosphates was prepared and characterized for future applications on the utilization of bioactivity and resorbability of Hydroxyapatite. Hydroxyapatite was synthesized by precipitation method using cuttlefish bone as the calcium (Ca) source in the form of calcium nitrate and ammonium phosphate as the phosphate (P) source. The property, crystal structure and crystalline size of samples were investigated by X-ray diffraction (XRD) and Fourier transforms infrared spectroscopy (FTIR). The results showed that the raw cuttlefish bone was calcite and aragonite phase and completely transformed to calcium oxide at 1000 °C. Hydroxyapatite from cuttlefish bone was hexagonal phase. The crystalline size of hydroxyapatite increases with increasing temperature from 200-1300 °C. This experiment indicated that the hydroxyapatite could be synthetized from cuttlefish bone.

Hydroxyapatite Production from Cuttlebone as Bone Scaffold Material Preparations

“…Produksi hidroksiapatit berbasis limbah cangkang sotong telah dilakukan beberapa penelitian di antaranya menggunakan metode kalsinasi dengan variasi suhu 900°C, 1.000°C, 1.100°C 3 jam dan perlakuan waktu Ball milling berkisar 1-3 jam (Solechan dan Rubijanto 2015), presipitasi kimia menggunakan HCl selama 10-12 jam (Faksawat et al 2015) dan hidrotermal pada suhu 200°C selama 24 jam (Tkalcec et al 2014), namun beberapa metode tersebut memiliki beberapa kelemahan yakni membutuhkan waktu kalsinasi yang cukup lama, sulit mendapatkan stoikiometri hidroksiapatit yang diinginkan dan masih terdapat unsur pengotor berupa ion karbonat. Berdasarkan permasalahan tersebut maka perlu dilakukan suatu upayah terkait penggunaan metode ekstraksi hidroksiapatit yang dapat menyerderhanakan kompleksnya produksi hidroksiapatit, salah satunya menggunakan kombinasi metode hidrotermal dan kalsinasi.…”

Faster Biomineralization and Tailored Mechanical Properties of Marine-Resource-Derived Hydroxyapatite Scaffolds with Tunable Interconnected Porous Architecture

“…Also, the presence of these ions is reported to facilitate rapid bone regeneration . Sea urchins ( Brissus latecarinatus ), sputnik sea urchin ( Phyllacanthus imperialis ), sea snail (Trochidae Infundibulum concavus ), eastern oyster ( Crassostrea virginica ), conch shells ( Strombus gigas ), giant clam ( Tridacna gigas ), Thunnus obesus bone, sea snail ( Cerithium vulgatum ), land snails ( Helix aspersa , Helix pomatia ), sea snail ( Rapana venosa ), and cuttlefish bone ( Sepia officinalis ) − are reported as natural resources of synthesis of HA for biomedical applications. Cuttlefish bone consists of two different layers, dorsal shield and lamellar matrix.…”

“…Some studies have reported that natural-resource-based raw products are expected to have trace elements like Mg 2+ , Na + , Zn 2+ , Sr 2+ , K + , and Al 3+ , which take part in bioactivity . Cuttlefish-derived HA containing these trace elements also supports faster biomineralization. − …”

Preparation of Nano-hydroxyapatite Obtained from Lates Calcarifer Fish Bone by Alkaline Hydrolysis Method

“…HAp can be obtained by two main ways: (1) synthesis from compounds containing calcium and phosphorus [6]; (2) extraction from natural sources including corals [11][12][13], cuttlefish bones [14][15][16][17][18], seashells from clams, oysters, snails [19][20][21][22][23], eggshell [24,25], mammalian bone (e.g. bovine [26,27], camel and horse [28]), fish scales [29][30][31][32], and fish bones [33][34][35][36].…”