Preparation and bioactivity of exopolysaccharide from an endophytic fungus Chaetomium sp. of the medicinal plant gynostemma pentaphylla

Zhang, Huiru; Wang, Xueqin; Li, Ruifang; Sun, Xueying; Sun, Siwen; Li, Qiang; Xu, Chunping

doi:10.4103/0973-1296.211033

Cited by 14 publications

(5 citation statements)

References 25 publications

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“…Ceratobasidium, Cladosporium, and Fusarium, which are closely related to the microbial community of medicinal Echinacea purpurea, are also present [14]. Chaetomium species are also found in many plants, such as Curcuma wenyujin, Platycladus orientalis, and Maytenus hookeri Loes [46,47]. In this work, S. suffruticosa was mainly collected from the Shell Islands of Yellow River Delta.…”

Section: Plos Onementioning

confidence: 95%

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Yao

et al. 2020

PLoS ONE

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Securinega suffruticosa (Pall.) Rehd is an excellent natural secondary shrub in the Shell Islands of Yellow River Delta. The roots of S. suffruticosa have high medicinal value and are used to treat diseases, such as neurasthenia and infant malnutrition. Any organism that is isolated from this species is of immense interest due to its potential novel bioactive compounds. In this research, the distribution and diversity of culturable endophytic fungi in S. suffruticosa were studied, and the endophytic fungi with antimicrobial activity were screened. A total of 420 endophytic fungi isolates were obtained from the S. suffruticosa grown in Shell Islands, from which 20 genera and 35 species were identified through morphological and internal transcribed spacer (ITS) sequence analyses. Chaetomium, Fusarium, Cladosporium, and Ceratobasidium were the dominant genera. The high species richness S (42), Margalef index D 0 (5.6289), Shannon-Wiener index H 0 (3.1000), Simpson diversity index D s (0.9459), PIE index (0.8670), and evenness Pielou index J (0.8719) and a low dominant index λ (0.0541) indicated the high diversity of endophytic fungi in S. suffruticosa, the various species of endophytic fungi with obvious tissue specificity. The inhibition percentages of the 12 species of such endophytic fungi against Colletotrichum siamense were 3.6%-26.3%. C. globosum, Fusarium sp.3, and C. ramotenellum had a high antibacterial activity against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) were between 0.5 mg/mL and 2 mg/mL. Alkaloid content detection indicated that endophytic fungi had a high alkaloid content, whereas the alkaloid contents of C. globosum and Fusarium sp.3 reached 0.231% and 0.170%, respectively. Members belonging to the endophytic fungal community in the S. suffruticosa of Shell Islands that may be used as antagonists and antibacterial agents for future biotechnology applications were identified for the first time.

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Section: Plos Onementioning

confidence: 95%

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Yao

et al. 2020

PLoS ONE

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“…The scavenging activity of EPS ranged from 67.80-81.80%, 60.60–81.20%, 70.40–84.70%, 78.40–88.50% against DPPH, OH − , Fe 2+ and NO, respectively. Zhang et al. (2017) revealed EPS as antioxidant agent that scavenged OH - and DPPH radicals.…”

Section: Resultsmentioning

confidence: 99%

Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues

Ogidi¹,

Ubaru²,

Ladi-Lawal³

et al. 2020

Heliyon

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Pleurotus spp. are white-rot fungi that utilize different agro-wastes to produce useful biologically active compounds. In this study, exopolysaccharides (EPS) were produced by Pleurotus pulmonarius in submerged culture supplemented with different agro-wastes. Functional groups in EPS were revealed using Fourier Transform-Infrared (FT-IR) spectroscopy. Antimicrobial activity of EPS was tested against microorganisms using agar well diffusion. Scavenging potentials of EPS was tested against 1, 1- diphenyl-2-picryhydrazyl (DPPH), hydroxyl (OH), iron (Fe 2+ ) and nitric oxide (NO) radicals. In vitro prebiotic activity of EPS was carried out. The highest yield (5.60 g/L) of EPS was produced by P. pulmonarius in submerged culture supplemented with groundnut shell (20.0 g/L). The functional groups in EPS were hydroxyl (-OH), methyl (-CH 3 ), ketone (-RCOH) and carbonyl group (-C=O). EPS displayed zones of inhibition (5.00–14.00 mm) against tested microorganisms. Scavenging activity of EPS ranged from 65.70-81.80% against DPPH. EPS supported the growth of Lactobacillus delbrueckii and Streptococcus thermophiles with values ranged from 3.04 × 10 4 –3.40 × 10 4 cfu/ml and 2.50 × 10 4 –2.81 × 10 4 cfu/ml, respectively. Submerged culture of P. pulmonarius with addition of agro-wastes enhanced yield of EPS. The EPS exhibited bio-functional properties like antimicrobial, antioxidant and prebiotic activities. Hence, agrowastes can be recycled in submerged fermentation with fungi to produce promising biomaterials for biopharmaceutical applications.

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“…CGMCC 5416 Phomochromanone A Anti-HIV-1 activity Yang et al ( 2020b ) 7 Phomochromanone B 8 Phomochromanone C Anti-PANC-1 cancer cells 9 Gynostemma Pentaphylla Chaetomium sp. JN180937.1 Exopolysaccharide (composed of glucose, mannose, arabinose, and galactose in the ratio of 78.29:8.99:8.64:4.08) Antioxidation and inhibition of cell proliferation Zhang et al ( 2017 ) 10 Duroia hirsuta Stelliosphaera formicum YU.101029 Stelliosphaerols A Anti- Staphylococcus aureus Forcina et al ( 2015 ) 11 Stelliosphaerols B 12 Vernonia amygdalina Curvularia papendorfii KR673909 Kheiric acid The anti-virus activities of human coronavirus 229E, feline coronavirus FCV F9, and Staphylococus sp. could inhibit the proliferation of human breast cancer MCF7 cells Khiralla et al ( 2020 ) 13 Markhamia platycalyx Aspergillus flocculus 5,9-Dihydroxy-2,4,6,8,10-pentamethyldodeca-2,6,10-trienal Anti-parasite activity of parasite Trypanosoma Bruce Tawfike et al ( 2019 ) 14 Extensive host plants Diaporthe vochysiae LGMF1583 Vochysiamides A Activity against gram-negative bacteria, Klebsiella pneumoniae Noriler et al ( 2019 ) 15 Paul...…”

Section: Research Progress On the Biosynthesis Of Natural Products By Endophytic Fungimentioning

confidence: 99%

Biotransformation ability of endophytic fungi: from species evolution to industrial applications

Liu

Zhou

Cui

et al. 2021

Appl Microbiol Biotechnol

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Increased understanding of the interactions between endophytic fungi and plants has led to the discovery of a new generation of chemical compounds and processes between endophytic fungi and plants. Due to the long-term co-evolution between fungal endophytes and host plants, endophytes have evolved special biotransformation abilities, which can have critical consequences on plant metabolic processes and their composition. Biotransformation or bioconversion can impact the synthesis and decomposition of hormones, sugars, amino acids, vitamins, lipids, proteins, and various secondary metabolites, including flavonoids, polysaccharides, and terpenes. Endophytic fungi produce enzymes and various bioactive secondary metabolites with industrial value and can degrade or sequester inorganic and organic small molecules and macromolecules (e.g., toxins, pollutants, heavy metals). These fungi also have the ability to cause highly selective catalytic conversion of high-value compounds in an environmentally friendly manner, which can be important for the production/innovation of bioactive molecules, food and nutrition, agriculture, and environment. This work mainly summarized recent research progress in this field, providing a reference for further research and application of fungal endophytes. Key points •The industrial value of degradation of endophytes was summarized. • The commercial value for the pharmaceutical industry is reviewed. Graphical abstract

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Preparation and bioactivity of exopolysaccharide from an endophytic fungus Chaetomium sp. of the medicinal plant gynostemma pentaphylla

Cited by 14 publications

References 25 publications

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues

Biotransformation ability of endophytic fungi: from species evolution to industrial applications

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