Preparation and application of a coated-fiber needle extraction device

Abstract

In this study, a needle-trap device with fibers coated with a molecularly imprinted polymer was developed for separation. A number of heat-resistant Zylon filaments were longitudinally packed into a glass capillary, followed by coating with a molecularly imprinted polymer. Then, the molecularly imprinted polymer coating was copolymerized and anchored onto the surface of the fibers. The bundle of synthetic fibers coated with the molecularly imprinted polymer was packed into a 21G stainless-steel needle and served as an extraction medium. The coated-fiber needle extraction device was used to extract volatile organic compounds from paints and gasoline effectively. Subsequently, the extracted volatile organic compounds were analyzed by gas chromatography. Calibration curves of gaseous benzene, toluene, ethylbenzene, and o-xylene in the concentration range of 1-250 μg/L were obtained to evaluate the method, acceptable linearity was attended with correlation coefficients above 0.998. The limit of detection of benzene, toluene, ethylbenzene, and o-xylene was 11-20 ng/L using the coated-fiber needle-trap device. The relative standard deviation of needle-to-needle repeatability was less than 8% with an extraction time of 20 min. The loss rates after storage for 3 and 7 days at room temperature were less than 30%.

Preparation and Application of Molecularly Imprinted Fiber-Fi Lled Needle Trap Extraction Device in Real Samples

“…The polymer solution was injected with a syringe directly into the filled glass tube, which was then sealed. The polymer solution was prepared according to described before [9]. After a reaction at 60 °C for 24 h, the glass tube was clamped with tweezers and washed with a solution of ethanol/NaOH (4:1, v/v) to remove the template molecule, i.e., benzoic acid.…”

“…In recent years, many micro sample preparation technologies and devices have been developed, such as solid-phase micro extraction technology (SPME) and so on [5,6]. Needle trap extraction (NTE) technology is a new type of sample preparation technology recently developed, especially with molecularly imprinted polymer (MIP) as an adsorption medium [7][8][9][10]. Technology can effectively concentrate VOCs in complex environments.…”

Molecularly imprinted polymer-based reusable biosensing device on stainless steel for spatially localized detection of cytokine IL-1β

“…Compared with biological counterparts (antibodies, enzymes and hormone receptors), better performances of MIP were demonstrated in high mechanical/chemical stability, low cost, and ease of preparation while having the similar affinity against the target analyte to that of polyclonal antibody [14]. Therefore, MIP has found wide applications in artificial mimics, sorbent assays, separation media, chemical sensing and catalysis [1,20,21,22]. Although MIP has been successfully developed against a wide range of small molecules, the imprinting of macromolecules such as proteins still presents a challenge [14] and so far, no MIP based biosensors for the detection of cytokines have been reported.…”