Preparation and application of a novel mixed-mode monolith for reversed-phase and per aqueous capillary electrochromatography

Tang, Sheng; Guo, Yong; Liang, Xiaojing; Fang, Wei; Yang, Limin; Liu, Shujuan; Liu, Xia; Jiang, Shengxiang

doi:10.1039/c5ay00611b

Cited by 5 publications

(3 citation statements)

References 34 publications

(40 reference statements)

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“…CEC separations of peptides and proteins have been performed in different separation modes, with reverse‐phase (RP) , affinity , mixed‐mode , and cationic or anionic ion‐exchange stationary phases, in classical FS capillary columns as well as in microfluidic chips . Attractive alternatives to the classical CEC columns packed with particulate materials are the monolithic stationary phases .…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…Rapid developments have been noticed with mixed‐mode organic–silica monoliths as they allow for more than one kind of separation capability using one single column. Among these are columns prepared from TMOS and 3‐chloropopyltrimethoxysilane (CPTMS) functionalized by nucleophilic substitution reactions with tertiary amines such as 4,5‐imidazoledicarboxylic acid , imidazole‐1‐acetic acid , 4,4’‐dipyridyl , o ‐phenanthroline , or histidine . For analysis of wide range of compounds these columns exhibited multiple retention mechanisms such as hydrophobic, hydrophilic, π–π and ion‐exchange interactions in reversed‐phase and per aqueous CEC.…”

Section: Organic–silica Hybrid Monolithsmentioning

confidence: 99%

Advances in the development and applications of organic–silica hybrid monoliths

Zajickova

2016

J of Separation Science

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This review will concentrate on recent progress (since 2013) toward preparation of organic-silica hybrid monoliths and their latest applications as extraction and separation media largely focusing on capillary liquid chromatography and capillary electrochromatography. Main emphasis will be given to advancement of approaches relying on the sol-gel chemistry of tetra- and tri-alkoxysilanes, sol-gel chemistry of alkoxysilanes and free-radical copolymerization with organic monomers, and free radical and ring-opening copolymerization of polyhedral oligomeric silsesquioxanes with organic monomers. Hybrid molecularly-imprinted polymer monoliths and hybrid monoliths made with non-silica-based precursors or in combination with metal alkoxides will be included as well.

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“…An alternative to these materials, hybrid organic-inorganic monoliths, has been developed via a sol-gel process, to combine the advantages of organic polymers and silica-based monoliths [37]. Such materials are mechanically stable, easy to fabricate, biocompatible, and easily permeable and can be employed in a wide pH range, so they have attracted much attention for application in several techniques including in-tube-SPME [38,39], nano-LC [40,41] and capillary electrochromatography [42,43].…”

Section: Introductionmentioning

confidence: 99%

Pipette tip micro‐solid phase extraction (octyl‐functionalized hybrid silica monolith) and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry to determine cannabidiol and tetrahydrocannabinol in plasma samples

Cruz

Miranda

Queiroz

2021

J of Separation Science

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describes the development of an innovative method to determine cannabinoids (cannabidiol and tetrahydrocannabinol) in human plasma samples by pipette tip micro-solid phase extraction and liquid chromatographymass spectrometry/mass spectromtery. An octyl-functionalized hybrid silica monolith, which had been synthesized and characterized, was used as a selective stationary phase. The octyl-functionalized hybrid silica monoliths presented high permeability and adequate mechanical strength. The micro-solid phase extraction variables (sample pH, draw-eject cycles, solvent for phase clean-up, and desorption conditions) were investigated to improve not only the selectivity but also the sorption capacity. The method was linear at concentrations ranging from the lower limit of quantification (10.00 ng/mL) to the upper limit of quantification (150.0 ng/mL). The lack of fit and homoscedasticity tests, as well as the determination coefficients (r 2 greater than 0.995), certified that linearity was adequate. The precision assays presented coefficient of variation values lower than 15%, and the accuracy tests provided relative error values ranging from 3.2 to 14%. Neither significant carry-over nor matrix effects were detected.Therefore, the pipette tip micro-solid phase extraction/liquid chromatographymass spectrometry/mass spectrometry method has demonstrated to be adequate to determine cannabidiol and tetrahydrocannabinol simultaneously in plasma samples for therapeutic drug monitoring of patients undergoing treatment with cannabinoids. K E Y W O R D Scannabidiol, octyl-functionalized hybrid silica monolith, pipette tip micro-solid phase extraction, plasma samples, tetrahydrocannabinol

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Preparation and application of a novel mixed-mode monolith for reversed-phase and per aqueous capillary electrochromatography

Abstract: A novel mixed-mode monolithic column was prepared and applied for reversed-phase and per aqueous capillary electrochromatography.

Cited by 5 publications

References 34 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Advances in the development and applications of organic–silica hybrid monoliths

Pipette tip micro‐solid phase extraction (octyl‐functionalized hybrid silica monolith) and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry to determine cannabidiol and tetrahydrocannabinol in plasma samples

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