Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Abstract

The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

Recent progress of sample stacking in capillary electrophoresis (2016–2018)

“…General reviews were aimed at enrichment techniques and enhancement of sensitivity in CE , their application in chiral CE , in CE‐MS and in the analysis of alkaloids . Electrophoretic stacking was also included in specialized reviews on CE analyses of peptides and proteins , pharmaceuticals , pesticides , antibiotics , food , and biologically important thiols . The use stacking by pH junction in CE‐ESI‐MS for proteomic analyses was reviewed in .…”

A capillary electrophoresis strategy to sensitively detect dynamic properties of coiled coil polypeptides

“…However, the current ensemble measurement of FRET is easily affected by non‐FRET signals, which results in the decrease of FRET efficiency [17,18]. To address this issue, CE was chosen to separate the NON‐FRET components before FRET was studied, which can enhance the sensitivity of FRET bioanalysis [19–21].…”

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015–mid 2018)

“…LC methods are currently preferred in proteomic or peptidomic studies but CE and microchip electrophoresis (MCE) techniques are also frequently employed in these fields as well as in the protein and peptide analyses in general . CE methods offer high resolution power, high separation efficiency, short analysis time, and require low amounts of samples and reagents .…”