Preparation and Amperometric Glucose Sensitivity of Covalently Bound Glucose Oxidase to (2-Aminoethyl)ferrocene on an Au Electrode

Abstract

A multilayer film consisting of glucose oxidase (GOx) and(2-aminoethyl)ferrocene (2-AF) as an electron mediator has been prepared on an Au electrode with the use of glutaraldehyde as a cross-linking agent The amount of GOx and 2-AF deposited on the electrode is varied by changing the concentration of 2-AF relative to that of GOx in the preparation bath, and the highest deposition is obtained at an optimum concentration of 2-AF. Interestingly, the molar ratio of 2-AF to GOx in the film is ~10, independent of relative compositions of 2-AF to GOx in

Layered Biomaterials on Electrode Supports: Routes to Electrochemical Biosensors, Immunosensors and DNA—Sensors

“…The magnitude of the amperometric responses of the multilayer enzyme electrodes resulting from the bioelectrocatalyzed oxidation of glucose in the presence of the diffusional electron mediator, ferrocene methanol, are controlled by the number of biocatalyst layers associated with the electrode. The one-step synthesis of a crosslinked electrically-contacted enzyme array on a Au surface was reported by Yoneyama et al [36]. Crosslinking of glucose oxidase and ferrocene ethylamine in the presence of glutaric dialdehyde leads to crosslinking between the ferrocene units and the protein, and parallel bridging of enzyme units.…”

Electrical contact of redox enzyme layers associated with electrodes: Routes to amperometric biosensors

“…The one-step synthesis of a crosslinked electrically contacted enzyme array on a Au-surface was reported by Yoneyama and coworkers [32]. Crosslinking of glucose oxidase and ferrocene ethylamine in the presence of glutaric dialdehyde leads to crosslinking between the ferrocene units and the protein, and parallel bridging of enzyme units.…”

Amperometric bienzyme glucose biosensor based on carbon nanotube modified electrode with electropolymerized poly(toluidine blue O) film

“…Since the first report on amperometric glucose biosensors [1], monolayer and multilayer enzyme electrodes have been widely employed as amperometric biosensors [2][3][4]. Various approaches have been employed for enzyme immobilization.…”