Layered Biomaterials on Electrode Supports: Routes to Electrochemical Biosensors, Immunosensors and DNA—Sensors

WILLNER, I.; Katz, Eugenii; Willner, Bilha

doi:10.1002/1616-8984(199904)5:1<45::aid-seup45>3.0.co;2-i

Cited by 14 publications

(5 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considerable research efforts have been devoted to combine enzyme-catalyzed reactions with electrochemical transducers to produce highly selective and sensitive biosensors [1][2][3][4][5][6][7][8][9]. These sensors are attractive for several reasons [10][11][12][13][14][15][16][17]: 1) complex organic molecules can be determined with the convenience, speed and ease that characterize amperometric measurements; 2) biocatalysts permit reactions to occur under mild conditions of temperature, pH and minimal substrate concentrations; 3) the sensor response is analyte dependent as dictated by the specificity of most enzyme reactions; and 4) the measured signal is inherently amplified by the enzyme catalytic cycle.…”

Section: Introductionmentioning

confidence: 43%

NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

et al. 2006

View full text Add to dashboard Cite

We present a carbon paste electrode (CPE) modified using the electron mediator bis(1,10-phenanthroline-5,6-dione) (2,2′-bipyridine)ruthenium(II) ([Ru(phend) 2+ exhibited luminescence even at low concentration. Modified CPEs were constructed and analyzed using cyclic voltammetry. The intercalated mediator remained electroactive within the layers (E°′ = −38.5 mV vs. Ag/AgCl, 3.5 M NaCl) and electrocatalysis of NADH oxidation was observed. The kinetics of the modified CPE shows a Michaelis -Menten behavior. This CPE was used for the oxidation of NADH in the presence of Bakers' yeast alcohol dehydrogenase. A calibration plot for ethanol is presented.

show abstract

Section: Introductionmentioning

confidence: 43%

NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

et al. 2006

View full text Add to dashboard Cite

show abstract

“…A variety of signal transduction techniques have been incorporated in the biosensor design, such as optical (Broude, 2002;Christel et al, 2002) and electrochemical transducer (Willner et al, 1999;Drummond et al, 2003;Fan et al, 2003;Boal and Barton, 2005;Wong and Gooding, 2006). Owing to the fact that electrochemical detectors are simple, portable, sensitive, fast response and low cost, electrochemical DNA biosensors have been recognized to be a promising way for diagnostic purpose as well as environmental and antibioterrorism monitoring Bard, 2003, 2004).…”

Section: Introductionsupporting

confidence: 48%

Sequence-specific detection of trace DNA via a junction-probe electrochemical sensor employed template-enhanced hybridization strategy

Zhang

Chen

et al. 2009

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…The assembly of monolayers or multilayers of biomaterials on electronic transducers, e.g., electrodes or piezoelectric crystals, is extensively employed for the development of biosensors. , Enzyme-layered electrodes, , antigen (or antibody) layers on electrodes or piezoelectric crystals, and nucleic acid-monolayer-functionalized electrodes were reported as active sensing interfaces for biosensors. A challenging topic in the development of biosensors relates to the sensitivity of the detection methods and to the need to develop amplification schemes for the analyses.…”

mentioning

confidence: 99%

Electrochemical and Quartz Crystal Microbalance Detection of the Cholera Toxin Employing Horseradish Peroxidase And GM1-Functionalized Liposomes

Willner²,

et al. 2001

View full text Add to dashboard Cite

An ultrasensitive method for the detection of the cholera toxin (CT) using electrochemical or microgravimetric quartz crystal microbalance transduction means is described. Horseradish peroxidase (HRP) and GM1-functionalized liposomes act as catalytic recognition labels for the amplified detection of the cholera toxin based on highly specific recognition of CT by the ganglioside GM1. The sensing interface consists of monoclonal antibody against the B subunit of CT that is linked to protein G, assembled as a monolayer on an Au electrode or an Au/ quartz crystal. The CT is detected by a "sandwich-type" assay on the electronic transducers, where the toxin is first bound to the anti-CT-Ab and then to the HRP-GM1ganglioside-functionalized liposome. The enzyme-labeled liposome mediates the oxidation of 4-chloronaphthol (2) in the presence of H 2 O 2 to form the insoluble product 3 on the electrode support or the Au/quartz crystal. The biocatalytic precipitation of 3 provides the amplification route for the detection of the CT. Formation of the insulating film of 3 on the electrode increases the interfacial electron-transfer resistance, R et , or enhances the electrode resistance, R′, parameters that are quantitatively derived by Faradaic impedance measurements and chronopotentiometric analyses, respectively. Similarly, the precipitate 3 formed on the Au/quartz crystal results in a mass increase on the transducer that is reflected by a decrease in the resonance frequency of the crystal. The methods allow the detection of the CT with an unprecedented sensitivity that corresponds to 1.0 × 10 -13 M.

show abstract

Layered Biomaterials on Electrode Supports: Routes to Electrochemical Biosensors, Immunosensors and DNA—Sensors

Cited by 14 publications

References 68 publications

NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

Sequence-specific detection of trace DNA via a junction-probe electrochemical sensor employed template-enhanced hybridization strategy

Electrochemical and Quartz Crystal Microbalance Detection of the Cholera Toxin Employing Horseradish Peroxidase And GM1-Functionalized Liposomes

Contact Info

Product

Resources

About