NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

Santiago-Berríos, Mitk’El B.; Vélez, Meredith M.; Borrero, Solmarie; Díaz, Agustín; Casillas, Craig A.; Hofmann, Cristina; Guadalupe, Ana R.; Colón, Jorge L.

doi:10.1002/elan.200503432

Cited by 42 publications

(60 citation statements)

References 51 publications

(101 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, there exist two kinds of catalytic model for the electrooxidation of NADH: one is a direct electro-catalysis 13,15,16,36 and the other is called a redox mediator electro-catalysis. [25][26][27][28][29][30][31][32]37 Though these redox mediators are very efficient electron shuttles for NADH oxidation processes, hydrodynamic voltammetry on the mediator-modified electrode remains a challenge for examining the kinetic parameters of NADH electrooxidation due to possible mediator loss from the electrode surface.…”

Section: 10mentioning

confidence: 99%

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Jin

Peng

et al. 2015

J. Electrochem. Soc.

View full text Add to dashboard Cite

In this study, the composite material that made up polyglycine/3d-4f hybrid-metallic cyano-bridged mixed coordination polymers (CyMCP) was successfully decorated onto a glassy carbon rotating disk electrode (GC RDE) using electrodeposition. The voltammetric behavior of NADH showed typical direct electrooxidation characteristics on the modified GC electrode. Our polyglycine/CyMCP-modified GC RDE was able to measure the apparent heterogeneous electron transfer rate constant of NADH during its direct electrooxidation by preventing fouling effects on the working electrode, which would generally cause a decrease in the oxidative peak current of NADH. In addition, differential pulse voltammetry provided a large linear range between the oxidative peak current and the concentration of NADH from 2-1500 μM at the polyglycine/CyMCP/GC electrode. Finally, the apparent heterogeneous electron transfer rate constant (k s NADH ) of NADH was determined to be 1.73 × 10 −2 cm · s −1 using the polyglycine/CyMCP-modified GC RDE. Both reduced and oxidized forms of β-nicotinamide adenine dinucleotide (NADH and NAD + , respectively) are the coenzymes for a large number of dehydrogenase enzymes (>300) and components of biomarker systems.1 In fact, because of its very important role in the field of analytical electrochemistry, 2,3 bioelectrocatalysis 4-6 and biofuel cells, 7,8 the electrochemical investigation for the formal potential of NADH/NAD + redox couple has attracted much attention. 9,10Therefore, direct oxidation of NADH at untreated or unmodified classical solid electrodes is known to appear poor reversibility and occurs with considerable overpotential (e.g., 1.1 V on carbon and 1.3 V on platinum). [11][12][13][14][15][16] Furthermore, accumulation of oxidation products involving radical intermediates, such as dimer from the one-electron oxidation product NAD +. , 17,18 can cause deactivation and fouling of the electrode surface. Such contamination of the working electrode can largely lower the sensitivity and reproducibility of voltammetric measurements. Consequently, considerable effort has been devoted with a strategy of surface modification on the substrate of conventional materials electrode to attain the electrode's long term stability for determination of NADH at a lower potential. 2,[19][20][21] Over the last few decades, various carbon nanomaterial 22-24 and redox-active mediator materials [25][26][27][28][29][30][31][32][33][34][35] have been modified onto the electrode surface in order to alleviate and/or prevent higher overpotential and fouling problems of the electrode. Therefore, there exist two kinds of catalytic model for the electrooxidation of NADH: one is a direct electro-catalysis 13,15,16,36 and the other is called a redox mediator electro-catalysis. [25][26][27][28][29][30][31][32]37 Though these redox mediators are very efficient electron shuttles for NADH oxidation processes, hydrodynamic voltammetry on the mediator-modified electrode remains a challenge for examining the kinetic parameters of NADH elect...

show abstract

Section: 10mentioning

confidence: 99%

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Jin

Peng

et al. 2015

J. Electrochem. Soc.

View full text Add to dashboard Cite

show abstract

“…Mediators have been immobilized directly on the electrode (Catalin Popescu et al 1999;Gligor et al 2009a;Jaegfeldt et al 1981;Persson and Gorton 1990) or on different supporting materials prior to mixing with the electrode material. As support materials, titanium phosphate (Kubota et al 2000), zirconium phosphates (Ladiu et al 2007;Santiago et al 2006), modified silica gel (de Lucca et al 2002;Santos et al 2002bSantos et al , 2003, and carbon nanotubes (Antiochia et al 2005;Huang et al 2007) have been used. Direct adsorption of the mediator cannot be used in technical applications due to a limited stability of the immobilized layers.…”

Section: Adsorptionmentioning

confidence: 99%

“…After 300 measurements, the residual activity was almost 95%. Layered α-zirconium phosphate (Zr(HPO 4 )2⋅H 2 O) was demonstrated to be a promising material for the immobilization of mediators (Santiago et al 2006). α-Zirconium phosphate is known to be a good ion exchanger because it is a strong acid material due to its phosphate groups and presents ion-conducting properties (Munteanu et al 2001).…”

Section: )mentioning

confidence: 99%

“…However, if the ratio of the catalytic current to the anodic current in the absence of NADH is to be considered, the sensors with MB showed the most beneficial characteristics (Vasilescu et al 2003). Interestingly, in another publication, a k obs value was estimated to be 5.7×10 4 M −1 s −1 using an immobilized ruthenium complex (Santiago et al 2006), which was much faster than in the solution (2.0×10 3 M −1 s −1 ) at comparable experimental conditions (Rivera et al 1994). Such difference in the catalytic activity occurs since the immobilized mediators are an integral part of the electrode surface.…”

Section: Comparison Of Processes With Dissolved and Immobilized Mediamentioning

confidence: 99%

“…Such difference in the catalytic activity occurs since the immobilized mediators are an integral part of the electrode surface. This part of the electrode surface enables electron hopping between mediators inside zirconium phosphate layers, which increases the reaction rate (Santiago et al 2006).…”

Section: Comparison Of Processes With Dissolved and Immobilized Mediamentioning

confidence: 99%

See 2 more Smart Citations

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Kochius

Magnusson

Hollmann

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The applicability of dissolved redox mediators for NAD(P) + regeneration has been demonstrated several times. Nevertheless, the use of mediators in solutions for sensor applications is not a very convenient strategy since the analysis is not reagentless and long stabilization times occur. The most important drawbacks of dissolved mediators in biocatalytic applications are interferences during product purification, limited reusability of the mediators, and their cost-intensive elimination from wastewater. Therefore, the use of immobilized mediators has both economic and ecological advantages. This work critically reviews the current stateof-art of immobilized redox mediators for electrochemical NAD(P) + regeneration. Various surface modification techniques, such as adsorption polymerization and covalent linkage, as well as the corresponding NAD(P) + regeneration rates and the operational stability of the immobilized mediator films, will be discussed. By comparison with other existing regeneration systems, the technical potential and future perspectives of biocatalytic redox reactions based on electrochemically fed immobilized mediators will be assessed.

show abstract

Drug Carriers Based on Zirconium Phosphate Nanoparticles

Colón¹,

Casañas²

2015

Tailored Organic‐Inorganic Materials

View full text Add to dashboard Cite

NADH Electrooxidation Using Bis(1,10-phenanthroline-5,6-dione)(2,2′-bipyridine)ruthenium(II)-Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes

Cited by 42 publications

References 51 publications

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Investigation of Direct Electrooxidation Behavior of NADH at a Chemically Modified Glassy Carbon Electrode

Immobilized redox mediators for electrochemical NAD(P)+ regeneration

Drug Carriers Based on Zirconium Phosphate Nanoparticles

Contact Info

Product

Resources

About