Electrical contact of redox enzyme layers associated with electrodes: Routes to amperometric biosensors

Willner, Itamar; Katz, Eugenii; Willner, Bilha

doi:10.1002/elan.1140091302

Cited by 191 publications

(92 citation statements)

References 46 publications

(23 reference statements)

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“…Amperometric biosensors are also considered Palmore and Whitesides (1994) After a brief introduction this is a summary of biofuel cell literature published between 1985 and 1992 on microbial and enzymatic fuel cells, including photomicrobial cells Willner et al (1997) Methods of contacting enzymes to biosensor electrodes, using monolayers, multilayers, reconstituted enzymes, and tethered cofactors Cosnier (1999) Biomolecule immobilisation on electrode surfaces by entrapment in or attachment to electropolymerised films. Mainly approached from a biosensor point of view Armstrong and Wilson (2000) Faradaic bioelectrochemistry including electron transfer techniques and kinetics at the protein-electrode interface, including promoters, SAMs and surfactant films Habermuller et al (2000) Electron transfer mechanisms in amperometric biosensors, covering many electron transfer pathways that are potentially of use in biofuel cell design Kano and Ikeda (2000) Fundamentals and practices of mediated bioelectrocatalysis.…”

Section: Applications Of Biofuel Cellsmentioning

confidence: 99%

Biofuel cells and their development

Bullen

Arnot

Lakeman

et al. 2006

Biosensors and Bioelectronics

939

545

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This review considers the literature published since 1994 on microbial and enzymatic biofuel cells. Types of biofuel cell are classified according to the nature of the electrode reaction and the nature of the biochemical reactions. The performance of fuel cells is critically reviewed and a variety of possible applications is considered. The current direction of development of biofuel cells is carefully analysed. While considerable chemical development of enzyme electrodes has occurred, relatively little progress has been made towards the engineering development biofuel cells. The limit of performance of biofuel cells is highlighted and suggestions for future research directions are provided.

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Section: Applications Of Biofuel Cellsmentioning

confidence: 99%

Biofuel cells and their development

Bullen

Arnot

Lakeman

et al. 2006

Biosensors and Bioelectronics

939

545

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show abstract

“…Willner and colleagues reported amperometric glucose sensors based on pyrroloquinoline quinone/glucose dehydrogenase SAMs that exhibited high current densities [15]- [19]. These surfaces are easily formed, particularly using alkane thiols and their derivatives on gold-coated surfaces.…”

Section: A Electrochemical Biomedical Sensing Technologiesmentioning

confidence: 99%

Emerging biomedical sensing technologies and their applications

2003

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Abstract-Recent progress in biomedical sensing technologies has resulted in the development of several novel sensor products and new applications. Modern biomedical sensors developed with advanced microfabrication and signal processing techniques are becoming inexpensive, accurate, and reliable. A broad range of sensing mechanisms has significantly increased the number of possible target measurands that can be detected. The miniaturization of classical "bulky" measurement techniques has led to the realization of complex analytical systems, including such sensors as the BioChemLab-on-a-Chip. This rapid progress in miniature devices and instrumentation development will significantly impact the practice of medical care as well as future advances in the biomedical industry. Currently, electrochemical, optical, and acoustic wave sensing technologies have emerged as some of the most promising biomedical sensor technologies. In this paper, important features of these technologies, along with new developments and some of the applications, are presented.

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“…Por essa razão, várias estratégias de imobilização tanto do mediador quanto da enzima sobre a monocamada têm sido desenvolvidas 117,118 . O contato elétrico entre a proteína redox e o eletrodo pode ser obtido pela ligação covalente do mediador à estrutura da enzima.…”

Section: Eletrodos Modificados Com Sam -Análise De Compostos Orgânicosunclassified

Emprego de monocamadas auto-organizadas no desenvolvimento de sensores eletroquímicos

Freire¹,

Pessôa²,

Kubota³

2003

Quím. Nova

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Recebido em 18/7/02; aceito 9/10/02 SELF-ASSEMBLED MONOLAYERS APPLICATIONS FOR THE DEVELOPMENT OF ELECTROCHEMICAL SENSORS. Selfassembled monolayers (SAMs) modified electrodes exhibit unique behavior that can greatly benefit electrochemical sensing. This brief review highlights the applications of SAM modified electrodes in electroanalytical chemistry. After a general introduction, which includes the approaches for SAM development, different electrochemical systems for detecting inorganic and organic species are described and discussed. Special attention to the coupling of biological sensing element to the SAM is given, which can selectively recognize the analyte. Future prospects are also evaluated.Keywords: chemically modified electrodes; self-assembled monolayers; electrochemical sensors. INTRODUÇÃOO desenvolvimento de sensores eletroquímicos é uma das áreas de maior e mais rápido crescimento dentro da Química Analítica, principalmente devido aos novos desafios impostos por amostras de interesse industrial, clínico e ambiental, que têm levado a uma crescente busca por sensores com melhores características, tais como alta sensibilidade, seletividade e estabilidade [1][2][3][4][5][6][7][8] . Apesar da grande versatilidade e perspectivas apresentadas pelos sensores eletroquímicos, a utilidade de um eletrodo é muitas vezes limitada devido a uma passivação gradual de sua superfície, que é conseqüência principalmente da adsorção dos produtos da própria reação de óxido-redução utilizada na detecção, ou ainda, dos sub-produtos destas reações que podem se polimerizar e se depositar sobre a superfície dos eletrodos 6 . Além disso, a sensibilidade de muitos analitos importantes pode ser prejudicada em função da cinética de transferência de elétrons entre estes compostos e os materiais dos eletrodos ser excessivamente lenta 9 . Uma outra limitação é a dificuldade de discriminar entre compostos alvos que possuam características redox similares 9 . Uma área que oferece grande potencial para minimizar os problemas acima descritos, e conseqüentemente para aumentar a aplicabilidade e eficiência dos sensores eletroquímicos, é a que compreende os chamados eletrodos quimicamente modificados (EQM) 9,10 . A habilidade para controlar e manipular deliberadamente as propriedades das superfícies dos eletrodos pode proporcionar uma variedade de efeitos atrativos, levando a superfícies com características que podem contornar efetivamente muitos dos problemas apresentados pelos sensores eletroquímicos tradicionais.Estudos experimentais, suportados pela teoria, têm mostrado que a velocidade de transferência de elétrons pode ser sensivelmente afetada em função da modificação da superfície dos eletrodos 11. A classificação das reações do eletrodo pode ser feita com base no grau de interação entre os reagentes e a superfície do eletrodo, ocorrida durante o estado de transição da transferência de elétrons 12 . Os processos de transferência de elétrons onde as interações entre as espécies reagentes e a superfície do eletrodo são fracas e não es...

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Electrical contact of redox enzyme layers associated with electrodes: Routes to amperometric biosensors

Cited by 191 publications

References 46 publications

Biofuel cells and their development

Biofuel cells and their development

Emerging biomedical sensing technologies and their applications

Emprego de monocamadas auto-organizadas no desenvolvimento de sensores eletroquímicos

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