Prenatal zinc supplementation to lipopolysaccharide infected female rats prevents neurochemical, behavioral and biochemical deficits produced in infants

Abstract

How to cite this article: Sharma N, Arora P, Nehru B. Prenatal zinc supplementation to lipopolysaccharide infected female rats prevents neurochemical, behavioral and biochemical deficits produced in infants. Neuroimmunol Neuroinflammation 2017;4:33-45. Aim:Recent research revealed an association between maternal infection i.e. lipopolysaccharide (LPS) exposure during pregnancy and increased risk for central nervous system disorders being passed onto the off-spring. Therefore, the present study was designed to investigate the effect of LPS infection during d14-17 of pregnancy (equivalent to third trimester in humans) on neurochemical, neurobehavioral abnormalities, biochemical as well as histopathological parameters in male/female pups. Also, the effect of zinc supplementation throughout pregnancy to female rats in ameliorating LPS induced neurodegenerative effects caused in pups were evaluated. Methods: Pregnant female rats were administered single dose of LPS (200 µg/kg) intraperitoneal on d14-17 of their pregnancy. Zinc supplementation was given throughout pregnancy (75 mg ZnSO 4 /L) in drinking water. Results: LPS injection to pregnant female rats significantly altered the levels of neurotransmitters (dopamine, serotonin and norepinephrine) in pups. Also, marked deterioration of motor behavior parameters (actophotometer, rotarod) as well as cognitive decline (plus maze and active avoidance) has been observed in male as well as female pups. Whereas, supplementation with zinc limited the alterations in behavioral parameters as well as significantly improved the level of neurotransmitters in prenatally exposed pups of both genders. However, levels of malondialdehyde and nitric oxide formed as well as antioxidant defense system including reduced glutathione, superoxide dismutase and catalase were found to be excessively compromised in female pups when compared to male pups. Conclusion: Hence, the study indicated LPS mediated toxicity in prenatally exposed pups is gender specific and zinc supplementation during pregnancy was found to attenuate LPS induced toxicity in pups. Key words:Neuroinflammation, lipopolysaccharide, motor dysfunction, cognitive decline, oxidative stress, cytokines, zinc sulphate ABSTRACTArticle history:

Zinc-nanoparticles alleviate the ovarian damage induced by bacterial lipopolysaccharide (LPS) in pregnant rats and their fetuses

“…A related study revealed that prenatally LPS-exposed rats resulted in a significant reduction in the levels of SOD and CAT in the midbrain of female offspring (Sharma et al. 2017 ). Furthermore, short- and long-term effects of LPS were found to induce endotoxemia in mouse ovarian tissue (Shokrizadeh et al.…”

“…who found that zinc supplementation during gestation can alleviate the oxidative stress in LPS maternally treated rats offspring (Sharma et al. 2017 ).…”

Study the Effect of Hemoxygenase-1 Induction and Suppression on Lipopolysaccharide- Brain Injury in Male Rats

“…Sharma et al, 2017 [44] , observed memory and behavioral impairments via active avoidance test in LPS treated rats, which escaped maximum number of trails in active avoidance test after prenatal LPS exposure as compared to the control rats.Also, many individuals have been observed for cognitive impairment in long term inflammatory conditions or treatment with cytokine based therapies.…”