Preliminary virulence genotyping and phylogeny of Escherichia coli from the gut of pigs at slaughtering stage in Brazil

Martins, Rodrigo Prado; Silva, Maria Cristina da; Dutra, Valéria; Nakazato, Luciano; Leite, Domingos da Silva

doi:10.1016/j.meatsci.2012.10.007

Cited by 6 publications

(5 citation statements)

References 41 publications

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“…The role of different animal species as asymptomatic carriers of EHEC/STEC has been extensively studied in the last years in Brazil. Besides cattle, which are their most common natural reservoir,173, 178 the presence of these pathogens has been identified in the feces of dairy buffaloes, 179 sheep,180, 181 pigs,182, 183 birds, 184 and fishes 185 . It is noteworthy that some relevant serotypes linked to human infections such as O103:H2 and O157:H7 have been recovered from the feces of sheep 186 and cattle 173 respectively.…”

Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning

confidence: 99%

Diarrheagenic Escherichia coli

Gomes

Elias

Scaletsky

et al. 2016

Brazilian Journal of Microbiology

384

353

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Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are collectively known as diarrheagenic E. coli. In this review, we have gathered information on current definitions, serotypes, lineages, virulence mechanisms, epidemiology, and diagnosis of the major diarrheagenic E. coli pathotypes.

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Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning

confidence: 99%

Diarrheagenic Escherichia coli

Gomes

Elias

Scaletsky

et al. 2016

Brazilian Journal of Microbiology

384

353

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“…Although very few studies have verified virulence genes in E . coli detected in the environment, equipment, and utensils in swine slaughterhouses/carcasses, some studies have detected these virulence genes in pig carcasses [ 107 – 110 ], suggesting that the virulence genes investigated in this study are circulating in E . coli strains in pigs.…”

Section: Discussionmentioning

confidence: 81%

“…The virulence genes present in isolates from slaughterhouses A and B were different, which can be attributed to the different batches of animals received for slaughter and different sanitary management in livestock animal farms [106]. Although very few studies have verified virulence genes in E. coli detected in the environment, equipment, and utensils in swine slaughterhouses/carcasses, some studies have detected these virulence genes in pig carcasses [107][108][109][110], suggesting that the virulence genes investigated in this study are circulating in E. coli strains in pigs. In addition to the potential public health risk related to food contamination, it is important to emphasize the economic loss due to infection by pathogenic E. coli strains in pigs (particularly piglets) and feed conversion reduction due to diarrheal symptoms, which may contaminate other pigs on the farm and cause death due to severe dehydration or the development of syndromes related to pathogenic E. coli strains [111].…”

Section: Plos Onementioning

confidence: 99%

Molecular characterization and biofilm-formation analysis of Listeria monocytogenes, Salmonella spp., and Escherichia coli isolated from Brazilian swine slaughterhouses

et al. 2022

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This study aimed to verify the presence of Listeria monocytogenes, Salmonella spp., and Escherichia coli in two Brazilian swine slaughterhouses, as well as to perform antibiograms, detect virulence and antimicrobial resistance genes, and evaluate the in vitro biofilm-forming capability of bacterial isolates from these environments. One Salmonella Typhi isolate and 21 E. coli isolates were detected, while L. monocytogenes was not detected. S. Typhi was isolated from the carcass cooling chamber’s floor, resistant to several antimicrobials, including nalidixic acid, cefazolin, chloramphenicol, doxycycline, streptomycin, gentamicin, tetracycline, and sulfonamide, and contained resistance genes, such as tet(B), tet(C), tet(M), and ampC. It also showed moderate biofilm-forming capacity at 37°C after incubating for 72 h. The prevalence of the 21 E. coli isolates was also the highest on the carcass cooling chamber floor (three of the four samplings [75%]). The E. coli isolates were resistant to 12 of the 13 tested antimicrobials, and none showed sensitivity to chloramphenicol, an antimicrobial prohibited in animal feed since 2003 in Brazil. The resistance genes MCR-1, MCR-3, sul1, ampC, clmA, cat1, tet(A), tet(B), and blaSHV, as well as the virulence genes stx-1, hlyA, eae, tir α, tir β, tir γ, and saa were detected in the E. coli isolates. Moreover, 5 (23.8%) and 15 (71.4%) E. coli isolates presented strong and moderate biofilm-forming capacity, respectively. In general, the biofilm-forming capacity increased after incubating for 72 h at 10°C. The biofilm-forming capacity was the lowest after incubating for 24 h at 37°C. Due to the presence of resistance and virulence genes, multi-antimicrobial resistance, and biofilm-forming capacity, the results of this study suggest a risk to the public health as these pathogens are associated with foodborne diseases, which emphasizes the hazard of resistance gene propagation in the environment.

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“…The role of different animal species as asymptomatic carriers of STEC/EHEC has been extensively studied in the last years in Brazil. In addition to cattle, which are their most common natural reservoir [18,19] the presence of these pathogens has been identified in the feces of dairy buffaloes [20], sheep [21,22], pigs [23,24], birds [25,26], and fishes [27]. It is noteworthy that some relevant serotypes linked to human infections such as O103:H2 and O157:H7 have been recovered from the feces of sheep and cattle [18,28].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Shiga Toxin-Producing Escherichia coli Immunodiagnostic Assay

et al. 2019

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Shiga toxin (Stx)–producing Escherichia coli (STEC) and its subgroup enterohemorrhagic E. coli are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal failure in children. Early diagnosis is essential for clinical management, as an antibiotic treatment in STEC infections is not recommended. Previously obtained antibodies against Stx1 and Stx2 toxins were employed to evaluate the sensitivity and specificity of the latex Agglutination test (LAT), lateral flow assay (LFA), and capture ELISA (cEIA) for STEC detection. The LAT (mAb Stx1 plus mAb stx2) showed 99% sensitivity and 97% specificity. Individually, Stx1 antibodies showed 95.5% and 94% sensitivity and a specificity of 97% and 99% in the cEIA and LFA assay, respectively. Stx2 antibodies showed a sensitivity of 92% in both assays and a specificity of 100% and 98% in the cEIA and LFA assay, respectively. These results allow us to conclude that we have robust tools for the diagnosis of STEC infections.

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Preliminary virulence genotyping and phylogeny of Escherichia coli from the gut of pigs at slaughtering stage in Brazil

Cited by 6 publications

References 41 publications

Diarrheagenic Escherichia coli

Diarrheagenic Escherichia coli

Molecular characterization and biofilm-formation analysis of Listeria monocytogenes, Salmonella spp., and Escherichia coli isolated from Brazilian swine slaughterhouses

Development and Validation of Shiga Toxin-Producing Escherichia coli Immunodiagnostic Assay

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