Preliminary studies on LTCC based PCR microreactor

Bembnowicz, Paweł; Małodobra, Małgorzata; Kubicki, Wojciech; Szczepańska, P.; Górecka-Drzazga, Anna; Dziuban, Jan; Jonkisz, Anna; Karpiewska, Anna; Dobosz, Tadeusz; Gołonka, Leszek

doi:10.1016/j.snb.2010.08.015

Cited by 30 publications

(28 citation statements)

References 18 publications

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“…The final antenna size is 13 × 16 × 2 mm using LTCC dielectric substrate (ε r = 6.7, σ = 0.0026 S/m). LTCC has been tested for biocompatibility and it has already been used in different biological applications [36,37]. The antenna geometry, with the main radiator positioned at the periphery, provides a wide internal space for future embedding of electronic integrated circuits.…”

Section: Methods and Simulation Setupmentioning

confidence: 99%

Detuning Study of Implantable Antennas Inside the Human Body

Vidal¹,

Curto²,

López-Villegas³

et al. 2012

PIER

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Abstract-This study quantifies the detuning and impedance mismatch of antennas implanted inside the human body. Maximum frequency shifts caused by variations in the electrical properties of body tissues and different anatomical distributions were derived. The results are relevant to the design of implantable antennas. They indicate the bandwidth enhancement and initial tuning necessary for correct functioning. The study was carried out using electromagnetic modeling based on the finite-difference time-domain method and highresolution anatomical models. Four anatomical computer models of two adults and two children were used. The implanted antennas operated in the Medical Implant Communication Service band. The most important detuning and impedance mismatch was found for subcutaneous locations and in areas where a layer of fat tissue was present. The maximum frequency shift towards higher frequencies was 70 MHz. The frequency shift did not occur symmetrically around 403 MHz, but was shifted towards higher frequencies.

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Section: Methods and Simulation Setupmentioning

confidence: 99%

Detuning Study of Implantable Antennas Inside the Human Body

Vidal¹,

Curto²,

López-Villegas³

et al. 2012

PIER

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“…Our lab is currently working on constructing a continuous flow PCR device [13] out of low temperature ceramic tape (LTCC). It has good thermal properties, affords easy integration of electrical and mechanical components, and fabrication of the fully integrated three-dimensional devices is relatively simple and inexpensive [13][14][15]. Once fired, the devices are highly resistant to chemical and thermal degradation [13,16].…”

Section: Introductionmentioning

confidence: 99%

“…However, it has been shown that not all LTCC is compatible with standard PCR reactions. When PCR is carried out in the presence of specific types of LTCC the reaction can be inhibited [14], which is also a problem found with silicon PCR devices [19][20][21][22]. This has been a major challenge in fabricating reliable PCR devices from LTCC and silicon.…”

Section: Introductionmentioning

confidence: 99%

Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

Besecker

Cornell

Hampikian

2013

Sensors and Actuators B: Chemical

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The increasing use of low temperature co-fired ceramic (LTCC) for the fabrication of biological microfluidic devices necessitates further research on LTCC biocompatibility. In this study we explore the inhibitory effect of DuPont's 951 LTCC on Polymerase Chain Reaction (PCR), and demonstrate a novel mechanism to increase biocompatibility between LTCC and PCR with the addition of a common passivation substance, bovine serum albumin (BSA). We show that DuPont's 951 LTCC binds negatively charged proteins including BSA and ovalbumin (OVA). This is a significant discovery as proteins (enzymes) are an essential component of most biological reactions, and a frequent addition to microfluidic devices. A proposed model for LTCC inhibition of PCR by enzyme adsorption is presented.

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“…Moreover, due to the high fusion temperature of materials involved in LTCC technology, up to 850ºC, they are preferable for high temperature applications over other multilayer technologies, such as those based on polymers. Several microreactors based on LTCC materials regarding nanoparticles synthesis [36][37][38], polymerase chain reaction systems [37], enzyme-based determinations [39] and biochemical diagnoses [40], among other applications, can be found in the bibliography. Herein, we propose the development of highly efficient ceramic microreactors to perform moderate or high temperature reactions.…”

Section: Introductionmentioning

confidence: 99%

Design, fabrication and characterization of microreactors for high temperature syntheses

Martínez-Cisneros

Pedro

Puyol

et al. 2012

Chemical Engineering Journal

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Microfluidic reactors offer many potential advantages in several research and industrial fields such as processes intensification, which includes a better reaction control (kinetics and thermodynamics), a high throughput and a safer operational environment (reduced manipulation of dangerous reagents and low sub-products generation). Nevertheless, scaling-down limitations appear concerning the materials used in the fabrication of microreactors for most of the liquid-phase reactions, since they usually require high temperatures (up to 300ºC), solvents and organic reagents. In this work, the development of a set of modular and monolithic microreactors based on the integration of microfluidics and a thermal platform (sensor/high-temperature heater) is proposed to perform high temperature reactions. The reliability and performance of both configurations were evaluated through an exhaustive characterization process regarding their thermal and microfluidic performance. Obtained results make the devices viable for their application in controlled and reproducible synthetic processes occurring at high temperatures such as the synthesis of quantum dots. The proposed microfluidic approach emerge as an engaging tool for processes intensification, since it provides better mass and temperature transfer than conventional methods with a reduction not only of the size and energy consumption, but also of by-products and reagents consumption.

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Preliminary studies on LTCC based PCR microreactor

Cited by 30 publications

References 18 publications

Detuning Study of Implantable Antennas Inside the Human Body

Detuning Study of Implantable Antennas Inside the Human Body

Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

Design, fabrication and characterization of microreactors for high temperature syntheses

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