Preliminary screening and immunogenicity analysis of antigenic epitopes of spring viremia of carp virus

Zhu, Bin; Zhang, Chen; Yang, Ben; Guo, Zi‐Rao; Zheng, Yonghua; Gong, Yu-Ming; Wang, Gao‐Xue

doi:10.1016/j.fsi.2019.09.063

Cited by 27 publications

(16 citation statements)

References 34 publications

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“…Additionally, the report indicates that the GCRV distributes in different tissues and organs of grass carp, particularly in the hepatopancreas, head kidney, dorsal muscle and spleen, especially it has the highest numbers of viral RNA copies in the head kidney after GCRV infection (Liang et al, ). Despite its great progress in vaccine control (Qiu et al, ), the frequent mutation of this RNA virus makes it more difficult to control than other types of pathogenic factors (Rao & Su, ; Zhu et al, ). At present, there is a shortage of effective anti‐GCRV agents and systematic research on pharmacology in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

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In vivo antiviral efficacy of moroxydine hydrochloride against grass carp reovirus and the drug safety assessment

Hao

Ling

et al. 2020

Aquac Res

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Our previous studies have verified that moroxydine hydrochloride (Mor) could inhibit replication of grass carp reovirus (GCRV) and suppress apoptosis of Ctenopharyngodon idella kidney cells, but be lack of information whether exists on antiviral activity in vivo. The paper was undertaken to explore the antiviral response of Mor against GCRV in grass carp and investigate the safety of drug for aquatic organisms. The results showed that injection treatment of Mor could more effectively inhibit GCRV replication than immersion administration. All the RNA systheses of vp3 and vp6 on day 7 in head kidney, gill, hepatopancreas and dorsal muscle in the Mor injection group were lesser than 0.07‐fold than that of in control group. And the GCRV‐inducing grass carp mortality was effectively controlled within 7 days post Mor injection therapy. Additionally, the reduction of superoxide dismutase activity, total antioxidant capacity and catalase activity in serum was effectively controlled by Mor. Moreover, drug safety assessment results showed that 500 mg/L of Mor was safe to C. idella, Bacillus subtilis, Chlorella vulgaris and Tetrahymena thermophila, which was far higher than the therapeutic concentration. The present study proved Mor as harmless formulations or products had potential value in the control of GCRV in aquaculture, with the advantage of super in vivo antiviral activity and environmental safety.

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Section: Introductionmentioning

confidence: 99%

“…mutation of this RNA virus makes it more difficult to control than other types of pathogenic factors (Rao & Su, 2015;Zhu et al, 2019).…”

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confidence: 99%

In vivo antiviral efficacy of moroxydine hydrochloride against grass carp reovirus and the drug safety assessment

Hao

Ling

et al. 2020

Aquac Res

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“…The dominating constituent of the humoral immune system of teleost fish is IgM (Tian et al, 2009) The bacterial ghost-loaded DNA vaccine in this study was established on the basis of pEG-G, its optimal immune concentration and immunization times are worth discussing. Several researches have indicated that the strength of immune response and immune protection is positively correlated with immune dose/concentration and times in a certain range (Zhao et al, 2020;Zhu, Liu, et al, 2014;Zhu et al, 2019). Therefore, we should further explore the optimal immersion concentration of pEG-G under different common carp specifications.…”

Section: Discussionmentioning

confidence: 99%

Immersion immunization of common carp with bacterial ghost‐based DNA vaccine inducing prophylactic protective immunity against spring viraemia of carp virus

Zheng

Zhang

et al. 2021

Journal of Fish Diseases

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Aquaculture products represent a key component of world trade (Naylor et al., 2000). According to the State of World Fisheries and Aquaculture 2020 report released by the FAO, the data showed that global aquaculture production reached 82.1 million tons in 2018, accounting for 46% of total aquatic products. Nevertheless, the frequent outbreak of infectious disease in aquatic animal has attracted more and more public attention on the development of aquaculture fishery (Gao et al., 2020). OIE has listed the spring viraemia of carp (SVC) as one of the animal diseases as notifiable (Ashraf et al., 2016). This disease usually breaks out with the spring and causes a large number of deaths in young fish once infected (Kanellos et al., 2006).Researchers have been working to find effective ways to prevent and control spring viraemia of carp virus (SVCV), but there is no approved treatment (Boonthai et al., 2017).

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“…The latter are produced in response to SVCV infection in carp (Sulimanovic 1973, Hill et al 1975, Fijan et al 1977 and to vesiculoviruses, the closest relatives to spriviviruses (Björklund et al 1996, Dietzgen et al 2017, which show evidence of immune selection in the G protein sequence (Kelley et al 1972, Vandepol et al 1986, Munis et al 2018. The region encoded by the Q1G target sequence (pre-cleavage amino acids 184−214) does not overlap with regions encoding neutralizing epitopes predicted for SVCV (Luo et al 2014, Zhu et al 2019 or vesicular stomatis virus (VSV) (Vandepol et al 1986, Keil & Wagner 1989, Munis et al 2018. Nevertheless, viral adaptation due to immune selection or other selection pressures acting in this region may pose a potential risk for future false negative results if nucleotide changes occur in the Q1G target sequence.…”

Section: Discussionmentioning

confidence: 99%

Analytical validation of two RT-qPCR tests and detection of spring viremia of carp virus (SVCV) in persistently infected koi Cyprinus carpio

et al. 2021

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Spring viremia of carp virus (SVCV) ia a carp sprivivirus and a member of the genus Sprivivirus within the family Rhabdoviridae. The virus is the etiological agent of spring viremia of carp, a disease of cyprinid species including koi Cyprinus carpio L. and notifiable to the World Organisation for Animal Health. The goal of this study was to explore hypotheses regarding inter-genogroup (Ia to Id) SVCV infection dynamics in juvenile koi and contemporaneously create new reverse-transcription quantitative PCR (RT-qPCR) assays and validate their analytical sensitivity, specificity (ASp) and repeatability for diagnostic detection of SVCV. RT-qPCR diagnostic tests targeting the SVCV nucleoprotein (Q2N) or glycoprotein (Q1G) nucleotides were pan-specific for isolates typed to SVCV genogroups Ia to Id. The Q2N test had broader ASp than Q1G because Q1G did not detect SVCV isolate 20120450 and Q2N displayed occasional detection of pike fry sprivivirus isolate V76. Neither test cross-reacted with other rhabdoviruses, infectious pancreatic necrosis virus or co-localizing cyprinid herpesvirus 3. Both tests were sensitive with observed 50% limits of detection of 3 plasmid copies and high repeatability. Test analysis of koi immersed in SVCV showed that the virus could be detected for at least 167 d following exposure and that titer, prevalence, replicative rate and persistence in koi were correlated significantly with virus virulence. In this context, high virulence SVCV isolates were more prevalent, reached higher titers quicker and persisted in koi for longer periods of time relative to moderate and low virulence isolates.

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Preliminary screening and immunogenicity analysis of antigenic epitopes of spring viremia of carp virus

Cited by 27 publications

References 34 publications

In vivo antiviral efficacy of moroxydine hydrochloride against grass carp reovirus and the drug safety assessment

In vivo antiviral efficacy of moroxydine hydrochloride against grass carp reovirus and the drug safety assessment

Immersion immunization of common carp with bacterial ghost‐based DNA vaccine inducing prophylactic protective immunity against spring viraemia of carp virus

Analytical validation of two RT-qPCR tests and detection of spring viremia of carp virus (SVCV) in persistently infected koi Cyprinus carpio

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