Preliminary molecular identification of cylindrospermopsin-producing Cyanobacteria in two Polish lakes (Central Europe)

Mankiewicz-Boczek, Joanna; Kokociński, Mikołaj; Gągała, Ilona; Pawełczyk, Jakub; Jurczak, Tomasz; Dziadek, Jarosław

doi:10.1111/j.1574-6968.2011.02451.x

Cited by 32 publications

(21 citation statements)

References 35 publications

(73 reference statements)

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“…Therefore, it is likely that CYN is widely distributed in European waters; however, the list of potential producers of this toxin remains incomplete. Moreover, no CYN-producing strains of C. raciborskii have yet been isolated, although it is a commonly occurring species in this region (Fastner et al 2003, 2007; Saker et al 2003; Bernard et al 2003; Haande et al 2008; Antal et al 2011; Mankiewicz-Boczek et al 2012). In Poland, CYN was first detected in 2006 in two shallow eutrophic lakes (Kokociński et al 2009), and preliminary molecular identification has shown that Aphanizomenon sp.…”

Section: Introductionmentioning

confidence: 99%

“…In Poland, CYN was first detected in 2006 in two shallow eutrophic lakes (Kokociński et al 2009), and preliminary molecular identification has shown that Aphanizomenon sp. appears to be the primary cyanobacterial genus responsible for the production of CYN in Polish lakes (Mankiewicz-Boczek et al 2012). Our recent study showed that C. raciborskii and Aphanizomenon spp.…”

Section: Introductionmentioning

confidence: 99%

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Aphanizomenon gracile (Nostocales), a cylindrospermopsin-producing cyanobacterium in Polish lakes

Kokociński

Mankiewicz-Boczek

Jurczak

et al. 2013

Environ Sci Pollut Res

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The cyanobacterial cytotoxin cylindrospermopsin (CYN) has become increasingly common in fresh waters worldwide. It was originally isolated from Cylindrospermopsis raciborskii in Australia; however, in European waters, its occurrence is associated with other cyanobacterial species belonging to the genera Aphanizomenon and Anabaena. Moreover, cylindrospermopsin-producing strains of widely distributed C. raciborskii have not yet been observed in European waters. The aims of this work were to assess the occurrence of CYN in lakes of western Poland and to identify the CYN producers. The ELISA tests, high-performance liquid chromatography (HPLC)-DAD, and HPLC-mass spectrometry (MS)/MS were conducted to assess the occurrence of CYN in 36 lakes. The cyrJ, cyrA, and pks genes were amplified to identify toxigenic genotypes of cyanobacteria that are capable of producing CYN. The toxicity and toxigenicity of the C. raciborskii and Aphanizomenon gracile strains isolated from the studied lakes were examined. Overall, CYN was detected in 13 lakes using HPLC-MS/MS, and its concentrations varied from trace levels to 3.0 μg L−1. CYN was widely observed in lakes of western Poland during the whole summer under different environmental conditions. Mineral forms of nutrients and temperature were related to CYN production. The molecular studies confirmed the presence of toxigenic cyanobacterial populations in all of the samples where CYN was detected. The toxicity and toxigenicity analyses of isolated cyanobacteria strains revealed that A. gracile was the major producer of CYN.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aphanizomenon gracile (Nostocales), a cylindrospermopsin-producing cyanobacterium in Polish lakes

Kokociński

Mankiewicz-Boczek

Jurczak

et al. 2013

Environ Sci Pollut Res

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“…CYN-producing Cylindrospermopsis from Australia and Asia have been reported, whereas Cylindrospermopsis strains isolated from Europe and America are incapable of CYN production (32)(33)(34)(35)(36). However, no conclusion can be drawn about the geographic distribution of the CYN-producing genotype of Cylindrospermopsis before additional samples from each continent are investigated by molecular and chemical methods.…”

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confidence: 97%

Sporadic Distribution and Distinctive Variations of Cylindrospermopsin Genes in Cyanobacterial Strains and Environmental Samples from Chinese Freshwater Bodies

Jiang

Xiao

et al. 2014

Appl Environ Microbiol

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e Increasing reports of cylindrospermopsins (CYNs) in freshwater ecosystems have promoted the demand for identifying all of the potential CYN-producing cyanobacterial species. The present study explored the phylogenetic distribution and evolution of cyr genes in cyanobacterial strains and water samples from China. Four Cylindrospermopsis strains and two Raphidiopsis strains were confirmed to produce CYNs. Mutant cyrI and cyrK genes were observed in these strains. Cloned cyr gene sequences from eight water bodies were clustered with cyr genes from Cylindrospermopsis and Raphidiopsis (C/R group) in the phylogenetic trees with high similarities (99%). Four cyrI sequence types and three cyrJ sequence types were observed to have different sequence insertions and repeats. Phylogenetic analysis of the rpoC1 sequences of the C/R group revealed four conserved clades, namely, clade I, clade II, clade III, and clade V. High sequence similarities (>97%) in each clade and a divergent clade IV were observed. Therefore, CYN producers were sporadically distributed in congeneric and paraphyletic C/R group species in Chinese freshwater ecosystems. In the evolution of cyr genes, intragenomic translocations and intergenomic transfer between local Cylindrospermopsis and Raphidiopsis were emphasized and probably mediated by transposases. This research confirms the existence of CYN-producing Cylindrospermopsis in China and reveals the distinctive variations of cyr genes.

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“…aphanizomenoides, and C. raciborskii, in line with our previous findings in Lake Karla . Recently it was shown that Aphanizomenon seems to be the main cyanobacterial genus responsible for the production of CYN in Polish lakes (Mankiewicz-Boczek et al, 2012). Evidences point to Aph.…”

Section: Mc-rrmentioning

confidence: 99%

Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake

et al. 2017

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Lake Karla (Central Greece) is a unique example -at European scale -of a shallow lake ecosystem that was dried in the 1960s and in 2009 started to be restored. The lake is listed in the network of the Greek protected areas as it is considered a vital aquatic ecosystem, in terms of biodiversity. It has, however, already been adversely affected by both agricultural and industrial land uses in the surrounding area, leading to eutrophication and shifting algal community towards bloom-forming toxic cyanobacterial species. After repeated heavyblooms, cyanotoxin occurrence and mass fish kills, the local ecosystem management authority has implemented a water quality monitoring program (July 2013 -July 2015) to assess environmental pressures and the response of aquatic biota in the lake. Microscopic, immunological, and molecular techniques combined with physico-chemical parameters, complemented by liquid chromatography tandem mass spectrometry (LC-MS/MS), were used to monitor cyanobacteria blooms and the associated cyanotoxin production from three different sites in Lake Karla and from the adjacent Kalamaki Reservoir. Water quality was also assessed by the structure of benthic invertebrate community on the sediment. Cyanobacteria were the main phytoplankton component, representing more than 70% of the total phytoplankton abundance; dominant taxa belonged to Cylindrospermopsis raciborskii, Limnothrix redekei, Anabaenopsis elenkinii, and Microcystis spp. Euglenophytes (Euglena), diatoms (Nitzschia), and chlorophytes (Scenedesmus) were also important phytoplankton constituents. LC-MS/MS confirmed the co-occurrence of microcystins, cylindrospermopsin, saxitoxin, neo-saxitoxin and anatoxin-a. The occurrence of cyanotoxins in relation to the persistent and dominant cyanobacteria and the impact of cyanobacterial harmful algal blooms on the newly constructed lake along with the land uses and the emergent mitigation measures are discussed.

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Preliminary molecular identification of cylindrospermopsin-producing Cyanobacteria in two Polish lakes (Central Europe)

Cited by 32 publications

References 35 publications

Aphanizomenon gracile (Nostocales), a cylindrospermopsin-producing cyanobacterium in Polish lakes

Aphanizomenon gracile (Nostocales), a cylindrospermopsin-producing cyanobacterium in Polish lakes

Sporadic Distribution and Distinctive Variations of Cylindrospermopsin Genes in Cyanobacterial Strains and Environmental Samples from Chinese Freshwater Bodies

Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake

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