Preliminary description of biocidal (syringomycin) activity in fluorescent plant pathogenic Pseudomonas species

Hu, F.-P.; Young, J. M.; Fletcher, Margaret J.

doi:10.1046/j.1365-2672.1998.00516.x

Cited by 28 publications

(12 citation statements)

References 15 publications

(25 reference statements)

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“…The three strains of phylogroup 11 tested here produced a toxin in the bioassays with G. candidum . It is likely that this toxicity is not due to syringomycin-like toxins as already shown by Hu et al [47]. Interestingly the cytochrome c oxidase operon present in P. cichorii was also found in the genome of phylogroup 7 strains (CC1582 and TA043) but in none of the other phylogroups (unpublished data).…”

Section: Resultssupporting

confidence: 57%

A User's Guide to a Data Base of the Diversity of Pseudomonas syringae and Its Application to Classifying Strains in This Phylogenetic Complex

Berge¹,

Monteil²,

et al. 2014

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The Pseudomonas syringae complex is composed of numerous genetic lineages of strains from both agricultural and environmental habitats including habitats closely linked to the water cycle. The new insights from the discovery of this bacterial species in habitats outside of agricultural contexts per se have led to the revelation of a wide diversity of strains in this complex beyond what was known from agricultural contexts. Here, through Multi Locus Sequence Typing (MLST) of 216 strains, we identified 23 clades within 13 phylogroups among which the seven previously described P. syringae phylogroups were included. The phylogeny of the core genome of 29 strains representing nine phylogroups was similar to the phylogeny obtained with MLST thereby confirming the robustness of MLST-phylogroups. We show that phenotypic traits rarely provide a satisfactory means for classification of strains even if some combinations are highly probable in some phylogroups. We demonstrate that the citrate synthase (cts) housekeeping gene can accurately predict the phylogenetic affiliation for more than 97% of strains tested. We propose a list of cts sequences to be used as a simple tool for quickly and precisely classifying new strains. Finally, our analysis leads to predictions about the diversity of P. syringae that is yet to be discovered. We present here an expandable framework mainly based on cts genetic analysis into which more diversity can be integrated.

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Section: Resultssupporting

confidence: 57%

A User's Guide to a Data Base of the Diversity of Pseudomonas syringae and Its Application to Classifying Strains in This Phylogenetic Complex

Berge¹,

Monteil²,

et al. 2014

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“…This study expands previous researches (Hu et al, 1998) highlighting that several strains of Pseudomonas cichorii, including the two used in this study (lCMP5707 and ICMP9417), were able to accumulate in culture low molecular weight diffusible toxins as well as non-dialysable syringomycin- like toxins. Their conclusion was mainly based on the results of extensive antifungal assays.…”

Section: Resultssupporting

confidence: 87%

“…In particular, P. cichorii is known to be the causal agent of "varnish spot", a serious disease that damages fieldgrown lettuce. Although previous studies (Shirata et at., 1983;Hu et at., 1998) had ascertained a biocidal activity in several strains of P. cichorii, no bioactive metabolite had been so far isolated and characterised. This bacterium is also known as the causal agent of "stem melanosis" on the cultivar Park of spring wheat (Triticum aestivum L.), a disease characterised by bleached empty heads and blackening of the rachids, peduncle and stem immediately beneath the nodes (Piening et at., 1985).…”

Section: Introductionmentioning

confidence: 99%

Toxic Metabolites and Lipopolysaccharides from Pseudomonas cichorii

Lazzaroni

Evidente

Surico³

2003

Pseudomonas Syringae and Related Pathogens

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Pseudomonas ciehorii is the causal agent of "varnish spot", a serious disease that damages field-grown lettuce. This paper reports preliminary results on the chemical and biological characterisation of the lipophylic phytotoxic and the hydrophilic antibiotic metabolites produced by a strain of P. ciehorii (ICMP5707), isolated in Germany from infected tissues of endive (Ciehorium endivia L.). These features, together with the results obtained on the chemical characterisation of some components of the lipopolysaccharides (LPS), contained in the outer membrane, were compared with those of a Canadian isolate (ICMP9417) from the eultivar Park of spring wheat (Triticum aestivum L.) and preliminarily classified as P. ciehorii. The initial results of this comparison indicated that the pattern of phytotoxic metabolites was similar for both strains, while strain ICMP5707 differs from strain ICMP9417 of P. ciehorii for the production of the antibiotic metabolites. In fact, the culture filtrates of strain ICMP9417, grown under identical conditions, do not show significant antibiotic activity. Moreover, preliminary investigations of the LPS of P. ciehorii ICMP9417 showed that the repeating unit of O-specific polysaccharide chain was certainly different from the one of P. ciehorii ICMP5707. Conversely the LPS lipid moiety is the same in the fatty acid composition.

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“…tolaasii and P. "reactans" (29,9) and this reaction results from a specific interaction between two diffusible lipodepsipeptides, tolaasin toxin by P. tolaasii (21,24) These isolates shared a very high similarity (of 100% for some of them) to the reference strain of P. tolaasii except one which scored 89% and was weakly pathogenic to A. bisporus . The two Pseudomonas isolates in group (II) which produced typical white line (Characteristic to P. "reactans") against P. tolaasii reference strain were identified as Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus

Abou-Zeid¹

2012

Braz. J. Microbiol.

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Twenty seven bacterial isolates were isolated from superficial brown discolorations on the caps of cultivated Agaricus bisporus. After White Line Assay (WLA) and the assist of Biolog computer-identification system, isolates were divided into groups: (I) comprised ninteen bacterial isolates that positively responded to a Pseudomonas "reactans" reference strain (NCPPB1311) in WLA and were identified as Pseudomonas tolaasii, (II) comprised two isolates which were WLA+ towards the reference strain (JCM21583) of P.tolaasii and were proposed to be P. "reactans". The third group comprised six isolates, two of which weakly responded to the strain of P. tolaasii and were identified as P. gingeri whereas the other four were WLAand identified as P. fluorescens (three isolates) and P. marginalis (one isolate). Isolates of P. tolaasii showed high aggressiveness compared with those of P. "reactans" in pathogenicity tests. Cubes of 1 cm 3 of A. bisporus turned brown and decreased in size when were inoculated with 10 µl of P. tolaasii suspension containing 10 8 CFU ml, whereas a similar concentration of P. "reactans" caused only light browning.Fifty µl of the same concentration of P. tolaasii isolates gave typical brown blotch symptoms on fresh mushroom sporophores whereas the two P. "reactans" isolates caused superficial light discoloration only after inoculation with 100 µl of the same concentration. Mixture from both bacterial suspensions increased the brown areas formed on the pileus. This is the first pathogenicity report of P. tolasii and P. "reactans" isolated from cultivated A. bisporus in Egypt.

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Preliminary description of biocidal (syringomycin) activity in fluorescent plant pathogenic Pseudomonas species

Cited by 28 publications

References 15 publications

A User's Guide to a Data Base of the Diversity of Pseudomonas syringae and Its Application to Classifying Strains in This Phylogenetic Complex

A User's Guide to a Data Base of the Diversity of Pseudomonas syringae and Its Application to Classifying Strains in This Phylogenetic Complex

Toxic Metabolites and Lipopolysaccharides from Pseudomonas cichorii

Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus

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