A User's Guide to a Data Base of the Diversity of Pseudomonas syringae and Its Application to Classifying Strains in This Phylogenetic Complex

Berge, Odile; Monteil, Caroline; Bartoli, Claudia; Chandeysson, Charlotte; Guilbaud, Caroline; Sands, David C.; Morris, Cindy E.

doi:10.1371/journal.pone.0105547

Cited by 208 publications

(402 citation statements)

References 51 publications

Supporting

Mentioning

381

Contrasting

Order By: Relevance

“…The P. syringae genealogy reconstructed from 1889 core gene loci (a total of 108 393 bp for which 38 484 were variable sites) confirmed genealogies previously inferred by MLSA and by previous core genome analysis (Berge et al , 2014). Importantly for the goal of this study, it also revealed recent common ancestry of all lineages within phylogroups 1a and 2d, respectively (Fig.…”

Section: Resultssupporting

confidence: 82%

Population-genomic insights into emergence, crop adaptation and dissemination of Pseudomonas syringae pathogens

et al. 2016

Self Cite

View full text Add to dashboard Cite

Many bacterial pathogens are well characterized but, in some cases, little is known about the populations from which they emerged. This limits understanding of the molecular mechanisms underlying disease. The crop pathogen Pseudomonas syringae sensu lato has been widely isolated from the environment, including wild plants and components of the water cycle, and causes disease in several economically important crops. Here, we compared genome sequences of 45 P. syringae crop pathogen outbreak strains with 69 closely related environmental isolates. Phylogenetic reconstruction revealed that crop pathogens emerged many times independently from environmental populations. Unexpectedly, differences in gene content between environmental populations and outbreak strains were minimal with most virulence genes present in both. However, a genome-wide association study identified a small number of genes, including the type III effector genes hopQ1 and hopD1, to be associated with crop pathogens, but not with environmental populations, suggesting that this small group of genes may play an important role in crop disease emergence. Intriguingly, genome-wide analysis of homologous recombination revealed that the locus Psyr 0346, predicted to encode a protein that confers antibiotic resistance, has been frequently exchanged among lineages and thus may contribute to pathogen fitness. Finally, we found that isolates from diseased crops and from components of the water cycle, collected during the same crop disease epidemic, form a single population. This provides the strongest evidence yet that precipitation and irrigation water are an overlooked inoculum source for disease epidemics caused by P. syringae.

show abstract

Section: Resultssupporting

confidence: 82%

Population-genomic insights into emergence, crop adaptation and dissemination of Pseudomonas syringae pathogens

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…2). The limited reliability of biochemical tests has been observed in other diagnostic and diversity studies, and our results confirm that biochemical data is not sufficient for distinguishing Australian BLS-associated isolates (Berge et al 2014). Considering further molecular characterisation was necessary, species-specific PCR and MLSA were evaluated.…”

Section: Discussionsupporting

confidence: 56%

Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia

Roach

Mann

Gambley³

et al. 2017

Eur J Plant Pathol

View full text Add to dashboard Cite

Several species of Xanthomonas cause bacterial leaf spot, a disease that affects solanaceous crops worldwide. The diversity of 64 Australian isolates of Xanthomonas spp. associated with bacterial leaf spot in tomato, capsicum and chilli crops in eastern Australia was determined using multi-locus sequence analysis of atpD, dnaK, efp and gyrB genes, species-specific PCR assays and biochemical analyses. At least five species of Xanthomonas associated with bacterial leaf spot were identified in Australian tomato, capsicum and chilli crops and their pathogenicity assessed. Phylogenetic and biochemical analyses identified X. euvesicatoria, X. perforans and X. vesicatoria as the most frequently recovered pathogenic species. Non-pathogenic and weakly pathogenic species were also identified. The suitability of the identification methods used and the implications of the detection of these species will be discussed.Keywords amylolytic . pectolytic . Solanaceae . disease management IntroductionBacterial leaf spot (BLS) is a disease of solanaceous crops that occurs worldwide, especially in warm and humid climates (Jones et al. 2014). Several species of Xanthomonas are reported to cause BLS of tomato (Solanum lycopersicum), capsicum and chilli (Capsicum annuum) . The symptoms of BLS are small, brown, angular, water-soaked lesions on leaves, stems and fruit, and result in defoliation and direct fruit damage. Severe infection may result in extensive damage to crops with significant yield losses (Pernezny et al. 2003). Species reported to cause BLS all produce similar symptoms on their hosts, making precise diagnosis difficult from visual symptoms alone. The impact of pathological convergence and importance of phylogenetic testing in the case of BLS are further highlighted by Hajri et al. (2009). The causal bacteria are spread by wind and water, and may survive in crop residues, weeds and volunteer plants (Jones et al. 1986). A link between field disease and seed contamination has Eur J Plant Pathol (2018) 150:595-608

show abstract

“…In some cases, damage caused by the Pssc was also expressed as yield losses, sometimes higher than 25 % (Table 1). Taken together, estimates of economic losses due to diseases caused by the Pssc on annual plants are fragmentary and expressed in DI disease incidence, P. s. Pseudomonas syringae a Phylogroups according to Berge et al (2014) b P. syringae pv. alisalensis has been also described as P. cannabina pv.…”

Section: Economic Importance Disease Outbreaks and Their Hotspotsmentioning

confidence: 99%

“…Integration of P. cichorii into the P. syringae species complex (hereafter Pssc) was later proposed (Bodilis and Barray 2006;Mulet et al 2010). Recently, Berge et al (2014) described 13 P. syringae phylogroups taking into account strains in the Pssc previously reported from crops as well as strains isolated from non-agricultural areas. They report equivalencies between genomospecies and phylogroups as well as the phylogenetic position of all pathovars for which phylogenetic information is available.…”

Section: Introductionmentioning

confidence: 99%

Insights into epidemiology and control of diseases of annual plants caused by the Pseudomonas syringae species complex

Lamichhane¹,

Messéan²,

Morris³

2015

J Gen Plant Pathol

Self Cite

View full text Add to dashboard Cite

The plant pathogenic Pseudomonas syringae species complex (Pssc) causes diseases of hundreds of species of monocots, herbaceous dicots and woody dicots worldwide. On annual plants, reports of disease caused by the Pssc have markedly increased in the last years. Hence, efforts are needed to mitigate the consequences of increasing disease outbreaks and to understand the underlying epidemiological factors. Here, we have reviewed the literature to highlight the salient features of the Pssc as pathogens of annual plant species and to underscore what remains unknown about the diseases they cause. The major points are as follows: (1) Since only the beginning of this century, 72 reports of disease outbreaks in 20 countries have been associated with the Pssc on over 40 annual plant species; (2) disease incidence caused by the Pssc on annual plants ranges from 50 to 100 % in epidemic years; (3) seed infestation is the main cause of long-distance pathogen dissemination; (4) foliar disease symptoms are very common on annual plants although the Pssc causes symptoms on fruits of bean, cantaloupe, cucumber, okra and tomato; (5) environmental factors such as rainfall and humidity have important roles in the Pssc disease epidemiology; (6) several new disease reports contradict the concept of the so-called pathovar, thereby raising the question of whether strains in the Pssc are mostly generalists rather than specialists as currently believed; and (7) disease control of annual plants is frequently based on approaches integrating biological, chemical and cultural measures with available plant resistance.

show abstract

A User's Guide to a Data Base of the Diversity of Pseudomonas syringae and Its Application to Classifying Strains in This Phylogenetic Complex

Cited by 208 publications

References 51 publications

Population-genomic insights into emergence, crop adaptation and dissemination of Pseudomonas syringae pathogens

Population-genomic insights into emergence, crop adaptation and dissemination of Pseudomonas syringae pathogens

Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia

Insights into epidemiology and control of diseases of annual plants caused by the Pseudomonas syringae species complex

Contact Info

Product

Resources

About