Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables

Ponce, Alejandra Graciela; Moreira, M.R.; Valle, C.E. del; Roura, Sara I.

doi:10.1016/j.lwt.2007.03.021

Cited by 73 publications

(51 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The effect of pH on bacteriocin activity in the CFS was evaluated by adjusting the pH from 1 to 10 with 1 M HCl or 1 M NaOH. After 2 h of incubation at 30°C, the samples were adjusted to pH 6.5 and the antimicrobial activity was determined (Ponce et al, 2008). The CFS was treated with proteinase K, α-chymotrypsine, and pronase E (final concentration 1 mg/ml) for 1 h, then the antimicrobial activity was measured by the AWDA method using L. monocytogenes as an indicator.…”

Section: Experimental Materials and Methodsmentioning

confidence: 99%

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

Morales-Estrada

López-Merino

Gutiérrez‐Méndez

et al. 2016

Polish Journal of Microbiology

View full text Add to dashboard Cite

A b s t r a c tDuring a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01 and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance, after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 but inactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1-9% NaCl concentration. The plate overlay assay showed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P. acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1). 280and Tetragenococcus halophilus) have previously been detected in Cotija cheese (Morales et al., 2011) without identification of those producing bacteriocins.The present work, aimed to identify the halotolerant lactic acid bacteria isolated from Cotija cheese and its antimicrobial activity. The bacteriocin produced by the isolate were also characterized. Experimental Materials and MethodsScreening for bacteriocin-producing lactic acid bacteria. Cotija cheese samples were collected from the mountain range of Michoacán, México as 20 g of cheese were added to 180 ml of peptone water. Then, the mixture was serially diluted with peptone water and plated on MRS and M-17 agar (Oxoid, Basingstoke, Hampshire, England) and supplemented with cycloheximide at 1 µg/ml to prevent growth of yeast and molds. The plates were incubated at 28°C for two to three days. The isolates were evaluated by cell morphology and Gram's stain reaction. The isolates were screened for bacteriocin production using the spot on the lawn assay using MRS plates (Moraes et al., 2010). Five µl of each isolated strain was spotted on MRS plates and incubated at 28°C for 24 h. After incubation, the plates were overlaid with 10 ml of BHI soft agar and inoculated with 10 5 CFU/ml of every tested pathogenic indicator strain. Then, the inhibition zone around the spots revealed potency of antibacterial activity.Biochemical identification of isolated bacterio cinproducers. Phenotypic identification was performed using Gram´s staining and an H 2 O 2 production test. Fermentation of carbohydrates was done by API 50 CHL system (BioMérieux, Inc,.Hazelwood Missouri, USA).Identification of the isolate by 16S rDNA sequencing and phylogenetic analysis. Bacteriocin-like substance producers were identified by 16S rDNA sequencing to confirm the results obtained from the biochemical identification. DNA was extracted using the modified method described by Wilson (2001)...

show abstract

Section: Experimental Materials and Methodsmentioning

confidence: 99%

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

Morales-Estrada

López-Merino

Gutiérrez‐Méndez

et al. 2016

Polish Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…), lignin, and protein, which can be metabolized by different microorganisms as a source of energy through enzymatic hydrolysis. While there is a wide diversity of microorganism producing hydrolytic enzymes, including bacteria, yeast, and fungi, the use of indigenous microorganisms (waste native microflora) ensures efficient activity, acting in an environment to which it is adapted (PONCE; MOREIRA; ROURA, 2008).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

et al. 2013

View full text Add to dashboard Cite

“…Organic acid produced by LAB leads to a reduction in pH levels and increases the production of hydrogen peroxide 19 . These products exhibit antibacterial activity against various pathogenic microorganisms, including Gram-positive and Gram negative bacteria 20 .…”

mentioning

confidence: 99%

Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens

Adeniyi¹,

Adetoye²,

Ayeni³

2015

Afr H. Sci.

View full text Add to dashboard Cite

Background: The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives. Objective: To determine the antimicrobial activity of cow's intestinal Lactic acid bacteria (LAB) against enteric commensals. Method: Escherichia coli, Klebsiella species (spp) and LAB were isolated from thirty different cow faecal samples and the LAB identified by partial sequencing of 16S rRNA. The antimicrobial activity of the LAB was determined against the test Escherichia coli and Klebsiella spp. Results: Five species of LAB were isolated from thirty cow faecal samples and identified as Enterococcus hirae (8), Enterococcus durans (6), Enterococcus faecium (1), Enterococcus faecalis (1) and Weissella confusa (1). Viable cells and cell free supernatant (CFS) of the LAB were able to inhibit the growth of the test organisms with the largest zone of inhibition by the viable cells being 26mm against Escherichia coli CB6 produced by Enterococcus hirae CO6A while Weissella confusa CO29M and Enterococcus hirae CO2A produced the largest zones of inhibition (26mm) Sci. 2015;15 (3):888-95. doi: http://dx.doi.org/10.4314/ahs.v15i3.24

show abstract

Preliminary characterization of bacteriocin-like substances from lactic acid bacteria isolated from organic leafy vegetables

Cited by 73 publications

References 32 publications

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens

Contact Info

Product

Resources

About