Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens

Abstract

Background: The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives. Objective: To determine the antimicrobial activity of cow's intestinal Lactic acid bacteria (LAB) against enteric commensals. Method: Escherichia coli, Klebsiella species (spp) and LAB were isolated from thirty different cow faecal samples and the LAB identified by partial sequencing of 16S rRNA. The antimicrobial activity of the LAB was determined against the test Escherichia coli and Klebsiella spp. Results: Five species of LAB were isolated from thirty cow faecal samples and identified as Enterococcus hirae (8), Enterococcus durans (6), Enterococcus faecium (1), Enterococcus faecalis (1) and Weissella confusa (1). Viable cells and cell free supernatant (CFS) of the LAB were able to inhibit the growth of the test organisms with the largest zone of inhibition by the viable cells being 26mm against Escherichia coli CB6 produced by Enterococcus hirae CO6A while Weissella confusa CO29M and Enterococcus hirae CO2A produced the largest zones of inhibition (26mm) Sci. 2015;15 (3):888-95. doi: http://dx.doi.org/10.4314/ahs.v15i3.24

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

“…According to [ 6 , 47 , 66 , [68] , [69] , [70] ] the antibacterial activities displayed by the antagonizing bacteria are as a result of production of antibacterial agents Surfactin and bacteriocin which include mersacidin and erisin are some of the antibacterial compounds produced by Bacillus species responsible for their antibacterial activities [ 23 , [71] , [72] , [73] , [74] ]. Brochocin-C is a bacteriocin produced by Brochothrix species, known for its broad spectrum activity [ 68 ].…”

Antimicrobial compounds produced by Enterococcus spp. isolates from fecal samples of wild South American fur seals

“…Only E. mundtii J5 produced antilisterial activity after the pH was adjusted. Other studies evaluating the bacteriocin-like substances from enterococci showed similar data to this (Birri et al, 2010;Adeniyi et al, 2015). Birri et al (2010) worked with the supernatant of 104 LAB study from the gastrointestinal tracts of children, and only one isolate of E. avium (0.96%) was a producer of an antagonist substance.…”

Beneficial Microbes: Roles in the Era of Antimicrobial Resistance

“…The antimicrobial activities of beneficial microorganisms particularly lactic acid bacteria isolated from various sources against pathogens have been reported by many authors [7,36].…”

“…This co-administration is done with the hope that this action will reduce antibiotic selective pressure, and decrease the emergence of drug-resistant pathogens. Currently, research is ongoing on the packaging of lyophilized lactic acid bacteria into capsules so that they can be used in the veterinary field (as probiotics) to inhibit the proliferation of zoonotic pathogens [36]. This method will limit the spread of diseases from animals to humans through animal-derived products.…”