Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

Mazzucotelli, Cintia Anabela; Ponce, Alejandra Graciela; Kotlar, Catalina Elena; Moreira, María del Rosario

doi:10.1590/s0101-20612013005000038

Cited by 43 publications

(43 citation statements)

References 27 publications

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“…Amylolytic activity: The obtained colonies were also screened for their ability to produce amylase on a selective medium containing 1% soluble starch as substrate as reported by Mazzucotelli et al [15]. After incubation, the plates were flooded with 1 % iodine in 2 % KI.…”

Section: Methodsmentioning

confidence: 99%

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Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Ethica¹,

Muchlissin²,

Saptaningtyas³

et al. 2018

Asian J. Chem.

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Hydrolytic bacteria are known for their crucial role in the bioconversion of organic matters mainly present in biomedical waste, making them advantageous in bioremediation. Analysis of abundance and colony diversity of culturable bacteria producing hydrolytic enzymes isolated from liquid biomedical waste of two different classes of hospitals in Semarang city, Central Java was carried out. Bacterial cultivation on nutrient agar slants resulted in total 26 viable colonies from inlet samples of Rumah Sakit Umum Roemani Muhammadiyah (R1) and Rumah Sakit Umum Daerah KRMT Wongsonegoro (R2) hospitals. Both hospitals represented two different classes of hospitals in Semarang (C and B, where B class is higher grade). Screening for production of four main hydrolytic enzymes (protease, lipase, amylase and cellulose) on all cultivable colonies obtained in this study. The results showed that all of them were capable of producing more than one type of hydrolytic enzymes. The composition was as follows: Protease producers (26 colonies), amylase producers (14 colonies), cellulose producers (13 colonies) and lipase producers (10 colonies).This study demonstrated that primary inlet of hospitals is a rich source of hydrolytic bacteria, where extracellular protease producers were found to be the main population of this group. In Semarang case, the inlet of lower class of hospital appeared to be more potential source of these hydrolytic bacteria than that of higher class one.

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Section: Methodsmentioning

confidence: 99%

“…After incubation, the plates were stained with Congo red dye and washed. A positive cellulase activity was detected by the formation of a yellow halo (8 mm or more) against a red background [15].…”

Section: Methodsmentioning

confidence: 99%

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Ethica¹,

Muchlissin²,

Saptaningtyas³

et al. 2018

Asian J. Chem.

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“…Consistently, in samples of the most popular fermented milk in Egypt, Lc. garvieae species were shown to be the dominant microflora (Mazzucotelli et al, 2013). Recently, Lc.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Lc. garvieae has also been isolated from faecal samples of healthy people, suggesting that this microorganism could be part of the human commensal microbiota or transient bacteria ingested with food (Mazzucotelli et al, 2013). Lc.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of the bacterial communities of bamboo shoots (Dendrocalamus latiflorus) during pickling process

Xia

Chunxia

et al. 2017

Int J of Food Sci Tech

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Summary The diversity and dynamics of the dominant bacterial communities arising during the pickling process of bamboo shoots (Dendrocalamus latiflorus) were investigated by nested polymerase chain reaction denaturing gradient gel electrophoresis combined with quantitative real‐time polymerase chain reaction. Results showed only several kinds of halophilic bacteria during early sampling time (0−3 days). After pickling for 7 days, Lactococcus lactis significantly increased (P < 0.05, quantities were 6.39 × 105 copies μL−1) and became the first dominant bacterium. After pickling for 14 days, Weissella sp. bands appeared and quickly became dominant on the 21st day (4.07 × 106 copies μL−1). As maturation progressed, Lc. lactis decreased in intensity whereas Weissella sp. increased in intensity. Finally, the quantities of Weissella sp. (2.50 × 107 copies μL−1) became higher than those of Lc. lactis (1.43 × 107 copies μL−1) after pickling for 56 days. The pickling process was initiated by Lc. lactis, followed by Lc. lactis and Weissella sp., and was finally succeeded by Weissella sp.

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“…Enterococcus is part of the intestinal microbiota of animals and has been used as an indicator of fecal contamination in environmental samples; in fermented corn mass, a strain was identified with low α-amylase activity (Kumar et al, 2012;Mazzucotelli et al, 2013;Montor-Antonio et al, 2014;Tambekar et al, 2016). It should be noted that all these genera have strains which produce amylolytic enzymes.…”

Section: Phylogenetic Classification Of the Library Sequences Obtainementioning

confidence: 99%

Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico

López

Ruiz

Sánchez³

et al. 2018

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Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing.Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch.Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter.

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Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

Cited by 43 publications

References 27 publications

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Protease Producers Predominate Cultivable Hydrolytic Bacteria Isolated from Liquid Biomedical Waste

Monitoring of the bacterial communities of bamboo shoots (Dendrocalamus latiflorus) during pickling process

Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico

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