The Atchafalaya River Basin in south-central Louisiana (USA) comprises the largest contiguous river-floodplain swamp in North America and functions as a distributary for the Mississippi River to the Gulf of Mexico. We assessed the impact of the annual flood pulse on fisheries production at a basin-wide scale. We modelled flood duration (days/year Butte LaRose gauge height >3.6 m) and magnitude (mean daily gauge height) against long term ) fishery-independent and fishery-dependent data on largemouth bass (Micropterus salmoides), crappie (Pomoxis spp.), blue catfish (Ictalurus furcatus), buffalofish (Ictiobus spp.), gizzard shad (Dorosoma cepedianum) and crayfish (Procambarus spp.). When the Atchafalaya River at Butte LaRose is at flood stage (≥ 3.6 m gauge height) for approximately 121-157 days/year, then annual relative abundances of largemouth bass, crappie, blue catfish and buffalofish are optimized during the fall (October-December). In contrast to the tenets of the flood pulse concept, gizzard shad abundance was optimized during low flow years, when flood duration is ≤10 days/year. Annual proportion of age-1 largemouth bass was associated positively with flow magnitude during the previous year, whereas proportions of age-2 largemouth bass and crappie were associated positively with flood magnitude 2 years prior to the fish sample. Results for commercial production metrics were ambiguous. Crayfish catches were associated positively with flood magnitude and duration, whereas gizzard shad landings peaked at a relatively small flood magnitude level and were not related to flood duration. Blue catfish and buffalofish annual landings were not influenced by the annual flood pulse. The annual flood pulse can be managed to optimize the availability of recreational (largemouth bass and crappie) and some commercial fisheries resources (blue catfish and crayfish). We estimate that a minimum discharge of 8807 m 3 s À1 is required at the river's source for approximately 4-5 months during the winter-spring months to optimize fisheries production. Figure 1. The Atchafalaya River Basin showing the study area and sample locations for the electrofishing, gill net and commercial crayfisher creel surveys. This figure is available in color online at wileyonlinelibrary.com/journal/rra MANAGING FLOODS TO OPTIMIZE ATCHAFALAYA BASIN FISHERIES Only stock-size fish were included (TL ≥ 200 mm for largemouth bass and 150 mm for crappie). b All buffalo species (Ictiobus spp.) were included. MANAGING FLOODS TO OPTIMIZE ATCHAFALAYA BASIN FISHERIES 283