Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time

Abstract

G6PDH-activity is a strong predictive marker of oocyte competence and may be useful in identifying oocytes with a good prognosis for further develop.

Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

“…Thereafter, numerous studies [8-12, 28-30, 32-35] including our own [14,16] have paid much interest to this method for assessing oocyte quality and evaluating the outcome of in vitro fertilization. However, the predictive value of the BCB approach used in these studies is still controversial [36].…”

“…The COCs were exposed to 26 mM of BCB dye diluted in modified phosphate buffer saline for 90 min at 38.5°C in humidified air atmosphere [16]. Following BCB staining, COCs were washed 3 times in modified phosphate buffer saline (mPBS).…”

“…Oocytes were classified as being in germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase-I (MI), and metaphase-II (MII) stages of the maturation process ( Fig. 1), as previously described [14,16].…”

“…Recently several studies [8][9][10][11][12] including our own [13][14][15][16], tried to introduce and highlight the usefulness of a test called brilliant cresyl blue (BCB) staining test for screening the most competent oocytes. The test is based on glucose-6-phosphate dehydrogenase (G6PDH) activity during oocyte development.…”

“…Using BCB test, meiotic competence, fertilization ability, potential of embryonic development, and relation of pro-and anti-apoptotic genes has been associated with G6PDH-activity in different animal models [8,9,14,16,18]. In most of these studies, the better performance of oocytes with low G6PDH-activity has been related to their better cytoplasmic maturation.…”