Association of glucose-6-phosphate dehydrogenase activity with oocyte cytoplasmic lipid content, developmental competence, and expression of candidate genes in a sheep model

Mohammadi‐Sangcheshmeh, Abdollah; Veshkini, Abbas; Hajarizadeh, Athena; Jamshidi-Adegani, Fatemeh; Zhandi, Mahdi; Abazari-Kia, Amir Hossein; Çınar, Mehmet Ulaş; Soleimani, Masoud; Gastal, E.L.

doi:10.1007/s10815-014-0264-6

Cited by 19 publications

(11 citation statements)

References 48 publications

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“…The activated oocytes were washed twice in CR1aa. Each group of 10 oocytes was cultured in 50 mL of CR1aa under mineral oil at 38.5 C, maximum humidity (98%), and 5% CO2 for 8 d [Mohammadi-Sangcheshmeh et al 2014]. To prevent toxic accumulation of ammonium, a half of the culture medium was substituted at 48 h intervals during the culture period.…”

Section: Parthenogenetic Activation and In Vitro Culturementioning

confidence: 99%

Impact of supplementary royal jelly onin vitromaturation of sheep oocytes: genes involved in apoptosis and embryonic development

Amiri

Deldar

Pirsaraei

2015

Systems Biology in Reproductive Medicine

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Optimizing culture conditions lead to the improvement of oocyte developmental competence and additives with anti-oxidative activity in culture media improved embryonic development. Royal jelly (RJ) is a product from the cephalic glands of nurse bees that has considerable health effects. The aim of this study was to investigate the effect of different concentrations of RJ on the maturation, cleavage, and blastocyst rates and gene expression in the oocyte and cumulus cells during in vitro maturation (IVM) of sheep oocyte. IVM of oocyte was performed in the presence of control (RJ0), 2.5 (RJ2.5), 5 (RJ5), 10 (RJ10), 20 (RJ20), and 40 (RJ40) mg/mL of RJ. Following the maturation period, parthenogenetic activation was carried out in two treatment groups (RJ0 and RJ10) and embryonic development was examined three and eight days thereafter. Moreover, the relative expression of BCL2 and BAX in oocyte as well as BCL2, BAX, HAS2, PTGS2, and STAR in cumulus cells were assessed. The results indicated that the addition of 10 mg/mL of RJ (90 ± 4.51%) to the maturation medium linearly increased the oocyte maturation rate compared to the control group (57 ± 2.42%), then it remained constant to the RJ40 (93 ± 3.10%) group. The higher RJ concentrations were associated with increased (p < 0.01) cleavage (53.3 ± 1.55% to 82.3 ± 2.82%) and blastocyst rate (15.5 ± 1.16% to 33.8 ± 3.09%) from the RJ0 to the RJ10 group. The relative mRNA expression of BCL2 and BAX in the oocyte was higher at RJ10. In cumulus cells, the expression of BCL2 was not affected, but that of BAX decreased, and expression of HAS2, PTGS2, and STAR were increased following the addition of RJ to the maturation media. In conclusion, the addition of 10 mg/mL of RJ to maturation medium improved blastocyst formation and decreased the apoptotic incidence in sheep cumulus cells and the oocyte during the in vitro development.

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Section: Parthenogenetic Activation and In Vitro Culturementioning

confidence: 99%

Impact of supplementary royal jelly onin vitromaturation of sheep oocytes: genes involved in apoptosis and embryonic development

Amiri

Deldar

Pirsaraei

2015

Systems Biology in Reproductive Medicine

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“…In addition, the diameter of the oocytes grown in “AF‐GCs add OGCs” was significantly greater than that of oocytes developed in “EAFs‐GCs add OGCs” and “natural OGCs” (Table ). As lipid content in oocytes is a biomarker of oocyte ability, we examined the lipid content in the oocytes, and we found significantly higher lipid content in oocytes grown in both “EAF‐GCs add OGCs” and “AF‐GCs add OGCs” compared with the natural counterparts (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…Another major impact of GCs addition to oocyte was the high lipid content in the oocytes. Lipid content is a simple but important oocyte marker associated with high developmental abilities . Many factors have been reported to affect lipid content in oocytes; for example, higher lipid content in culture medium increased lipid content in oocyte and GCs .…”

Section: Discussionmentioning

confidence: 99%

Addition of granulosa cells collected from differential follicle stages supports development of oocytes derived from porcine early antral follicles

Ishiguro

Munakata

Shirasuna

et al. 2018

Reprod Medicine & Biology

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PurposeImprovement of in vitro oocyte growth by addition of granulosa cells derived from differential developmental stages of follicles.MethodsGranulosa cells (GCs) collected from either early antral follicles (EAFs) or antral follicles (AFs) were added to oocyte‐granulosa cell complexes (OGCs) derived from EAFs, and the in vitro growth of the oocytes was evaluated.ResultsGranulosa cells were incorporated into OGCs to form new OGCs within 2 days of culture. After 14 days of culture, the number of GCs surrounding oocytes was similar among the three OGCs conditions (unmanipulated “natural OGCs,” “EAF‐GCs add OGCs,” and “AF‐GCs add OGCs”), whereas the survival rate of the GCs and diameter of oocytes grown in vitro were the greatest for “AF‐GCs added OGCs.” After parthenogenetic activation, developmental rate till the blastocyst stage tended to be higher for “AF‐GCs add OGCs” compared with other groups. Addition of AF‐GCs significantly increased a hypoxic marker (pimonidazole staining) and increased the lipid content in oocytes grown in vitro compared with unmanipulated OGCs.ConclusionAddition of GCs derived from more advanced stages of follicles to the OGCs changes the metabolism of oocytes and is beneficial for in vitro growth of oocytes derived from EAFs.

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“…Therefore, BCB staining has been widely used to select competent oocytes in mouse [19,22], sheep [23], goat [24], and cattle [25]. Studies have revealed that the embryos derived from (BCB + ) cumulus-oocyte complexes (COCs) had higher developmental competency than embryos derived from (BCB -) COCs [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Increase of Reactive Oxygen Species Associates with the Achievement of Meiotic Competency in Rat Oocytes Cultured In Vitro

Tiwari

Chaube

2017

ROS

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Reactive oxygen species (ROS) play an important role during meiotic maturation and ovulation. The downstream impact of ROS during the achievement of meiotic competency remains ill understood. The present study was aimed to find out the impact of ROS on the level of cyclic nucleotides and maturation promoting factor (MPF) during the achievement of meiotic competency. For this purpose, cumulus oocyte complexes (COCs) were collected from the ovary of experimental rats injected with 20 IU pregnant mare's serum gonadotropin (PMSG) for 48 h, and PMSG was followed by 20 IU human chorionic gonadotropin (hCG) for 14 h. The morphological changes, meiotic status of oocyte, brilliant cresyl blue (BCB) staining for meiotic competency, total ROS, hydrogen peroxide (H2O2), adenosine 3′,5′-cyclic monophosphate (cAMP), guanosine 3′,5′-cyclic monophosphate (cGMP), cell division cycle 25B (Cdc25B), Wee1, specific phosphorylation status of cyclin-dependent kinase 1 (Cdk1), and cyclin B1 expression levels were analyzed. Data suggest that the culture of diplotene-arrested COCs in vitro resulted in spontaneous as well as hCGinduced meiotic exit from diplotene arrest (EDA) in a time-dependent manner, but the first polar body (PB-I) was not extruded. However, 20 IU hCG surge induced extrusion of PB-I in majority of ovulated oocytes. A moderate increase of total ROS as well as H2O2 levels associate with EDA. The cAMP, cGMP, Cdc25B, Thr161 phosphorylated Cdk1 as well as cyclin B1 levels were decreased, while Wee1 and Thr14/Tyr15 phosphorylated Cdk1 levels were significantly increased leading to MPF destabilization. The destabilized MPF resulted in spontaneous EDA in rat COCs cultured in vitro. Taken together, these data suggest that a moderate increase of ROS decreases the cyclic nucleotides level that destabilizes MPF. The destabilized MPF results in EDA in rat COCs cultured in vitro.

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Association of glucose-6-phosphate dehydrogenase activity with oocyte cytoplasmic lipid content, developmental competence, and expression of candidate genes in a sheep model

Abstract: Activity of G6PDH in sheep oocytes is highly associated with lipid content, and compared with the morphological parameters might be a more precise and objective predictor for subsequent developmental competence in vitro.

Cited by 19 publications

References 48 publications

Impact of supplementary royal jelly onin vitromaturation of sheep oocytes: genes involved in apoptosis and embryonic development

Impact of supplementary royal jelly onin vitromaturation of sheep oocytes: genes involved in apoptosis and embryonic development

Addition of granulosa cells collected from differential follicle stages supports development of oocytes derived from porcine early antral follicles

Increase of Reactive Oxygen Species Associates with the Achievement of Meiotic Competency in Rat Oocytes Cultured In Vitro

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