Precise determination of N‐acetylcysteine in pharmaceuticals by microchip electrophoresis

Abstract

A novel microchip electrophoresis method for the rapid and high-precision determination of N-acetylcysteine, a pharmaceutically active ingredient, in mucolytics has been developed. Isotachophoresis separations were carried out at pH 6.0 on a microchip with conductivity detection. The methods of external calibration and internal standard were used to evaluate the results. The internal standard method effectively eliminated variations in various working parameters, mainly run-to-run fluctuations of an injected volume. The repeatability and accuracy of N-acetylcysteine determination in all mucolytic preparations tested (Solmucol 90 and 200, and ACC Long 600) were more than satisfactory with the relative standard deviation and relative error values <0.7 and <1.9%, respectively. A recovery range of 99-101% of N-acetylcysteine in the analyzed pharmaceuticals predetermines the proposed method for accurate analysis as well. This work, in general, indicates analytical possibilities of microchip isotachophoresis for the quantitative analysis of simplified samples such as pharmaceuticals that contain the analyte(s) at relatively high concentrations.

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

“…The well‐resolved separation of 9 AAs extracted from sand leachate was obtained. One can observe that ITP using a CE microchip and conductimetry was also used for acetyl cysteine quantification in pharmaceutics .…”

Analytical capillary isotachophoresis after 50 years of development: Recent progress 2014–2016

“…They demonstrated successful purification and preconcentration of the analyte from two proteins with similar isoelectric properties: from R‐phycoerythrin and albumin, a highly abundant serum protein (LE: 20 mM KOH, HEPES, 0.5 M urea, 1% Triton X‐100, 2% PVP, pH 8.0; TE: 60 mM histidine, HEPES, 0.5 M urea, 1% Triton X‐100, 2% PVP, pH 7.2). Rudašová and Masár described a method for the analysis of N‐acetylcysteine in mucolytics on a microchip with conductivity detection. The authors used 2‐aminobenzoic acid as internal standard and reached a LOD of about 8 mg/L (LE: 10 mM Cl − , 20 mM histidine, 0.1% MHEC, pH = 6.02; TE: 10 mM MES, 12 mM histidine, 0.1% MHEC, pH = 6.20).…”

Surface modification by deposition of IPA plasma and gellan gum/chitosan hybrid hydrogel onto thermoplastic polyurethane for controlled release of N‐acetylcysteine

“…N‐acetylcysteine demonstrated some notable activities including cell proliferation, glutathione levels, burn wound healing, inhibiting reactive oxygen species (·OH, ·NO 2 , and ·CO 3 − ..), and abating tobacco dependence . It is a cheap, harmless, convenient, and effective medicine for pharmacotherapy …”

“…[40][41][42][43][44] It is a cheap, harmless, convenient, and effective medicine for pharmacotherapy. [45][46][47][48] Recently, nonwoven fabric has been widely used as biomedical materials, biosensors, [8] and bioreactors, which has some advantages including resistant acid and base, large surface area, and mechanical strength. [49,50] In this study, thermoplastic polyurethane (TPU, advantages: weathering resistance, oil resistance, heat and aging resistance, water proof, transparent, and elasticity) nonwoven fabric is used as a drug carrier material.…”