Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

Poinsot, Véréna; Ong-Meang, Varravaddheay; Ric, Audrey; Gavard, Pierre; Perquis, Lucie; Couderc, François

doi:10.1002/elps.201700270

Cited by 28 publications

(9 citation statements)

References 119 publications

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“…The goal was to obtain an experimental protocol well suited for the quantification of AA involved in major depression, including Trp. The results were then compared with those obtained by CE/C 4 D, a commonly used technique for label-free AA analysis, which therefore requires no derivatization (see [20] for a review). Finally, and it is totally new, we show here that Trp-CBQCA was detected in plasma at physiological levels but could not be reliably quantified.…”

Section: Introductionmentioning

confidence: 99%

3‐(4‐Carboxybenzoyl)quinoline‐2‐carboxaldehyde labeling for direct analysis of amino acids in plasma is not suitable for simultaneous quantification of tryptophan, tyrosine, valine, and isoleucine by CE/fluorescence

Perquis

Sarazin³

et al. 2021

Electrophoresis

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Capillary electrophoresis coupled to LED-induced fluorescence detection is a robust and sensitive technique used for amino acids (AA) analysis in biological media, after labeling with 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA). We wanted to quantitate in plasma tryptophan (Trp), tyrosine (Tyr), valine (Val), and isoleucine (Ile). Among the different labeled AA-CBQCA, Trp has the lowest fluorescence yield, which makes its detection and quantification very difficult in biological samples such as plasma. We tried to improve Trp analysis by CE/LED-induced fluorescence detection to its maximal sensitivity by using large volume sample stacking as a preconcentration step in our analytical protocol. At pH 9.5, this step caused a drop in resolution during the separation of the four AAs and it was therefore necessary to work at pH 10. We have found that Tyr, Val, Ile, and Trp are detected and well separated from the other AAs, but Trp cannot be quantified in plasma samples, mainly because of the low fluorescence yield of the Trp-CBQCA derivative. The recorded LOD is 0.18 μM for Trp-CBQCA in standard solution with a resolution between Trp and Tyr of 1.2, while the LOD is 6 μM in plasma with the same resolution. Trp, Tyr, Val, and Ile are, however, efficiently quantified when using a 3 M acetic acid electrolyte and CE associated with capacitively coupled contactless conductivity detection, which also has the advantage of not requiring derivatization or large volume sample stacking. This article demonstrates, for the CE user, that quantitative analysis of these four AA in mouse plasma can be performed by CE-fluorescence after CBQCA labeling, with the exception of Trp. It can be advantageously replaced by CE/capacitively coupled contactless conductivity detection, the only efficient one for Trp, Tyr, Val, and Ile quantification. In this case, the LOD for Trp is 2 μM. The four AAs are separated with resolution with neighbors above 1.5.

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Section: Introductionmentioning

confidence: 99%

3‐(4‐Carboxybenzoyl)quinoline‐2‐carboxaldehyde labeling for direct analysis of amino acids in plasma is not suitable for simultaneous quantification of tryptophan, tyrosine, valine, and isoleucine by CE/fluorescence

Perquis

Sarazin³

et al. 2021

Electrophoresis

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“…From the listed separation techniques, our review is focused on the determination of AAs using CE. High application potential of CE for analysis of AAs is illustrated by variety of reviews covering periodically the published articles in this area [3‐7]. In addition to high separation efficiency, CE offers benefits, such as the low consumption of samples, BGE, and washing solutions, and consequently has a low production of waste.…”

Section: Introductionmentioning

confidence: 99%

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Celá

Glatz

2020

Electrophoresis

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Amino acids are essential compounds for living organisms, and their determination in biological fluids is crucial for the clinical analysis and diagnosis of many diseases. However, the detection of most amino acids is hindered by the lack of a strong chromophore/fluorophore or electrochemically active group in their chemical structures. The highly sensitive determination of amino acids often requires derivatization. Capillary electrophoresis is a separation technique with excellent characteristics for the analysis of amino acids in biological fluids. Moreover, it offers the possibility of precapillary, on‐capillary, or postcapillary derivatization. Each derivatization approach has specific demands in terms of the chemistry involved in the derivatization, which is discussed in this review. The family of homocyclic o‐dicarboxaldehyde compounds, namely o‐phthalaldehyde, naphthalene‐2,3‐dicarboxaldehyde, and anthracene‐2,3‐dicarboxaldehyde, are powerful derivatization reagents for the determination of amino acids and related compounds. In the presence of suitable nucleophiles they react with the primary amino group to form both fluorescent and electroactive derivatives. Moreover, the reaction rate enables all of the derivatization approaches mentioned above. This review focuses on articles that deal with using these reagents for the derivatization of amino acids and related compounds for ultraviolet‐visible spectrometry, fluorescence, or electrochemical detection. Applications in capillary and microchip electrophoresis are summarized and discussed.

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“…Capillary electrophoresis (CE) is a powerful method for the separation and determination of various analytes such as amino acids, amines, steroids, nucleobases, nucleosides, nucleotides, peptides, proteins, and carbohydrates . Its separation efficiency is significantly higher compared to high performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography

Кравченко

Колобова

Карцова

2020

Separation Science Plus

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In this study, the preparation process of multifunctional coating based on imidazole and β-cyclodextrin has been developed. The synthesis includes several steps: activation of fused silica capillary walls via alkali, then process of silylation and finally functionalization with imidazole and β-cyclodextrin derivative. The latter step was carried over 22 h at 100 • C at 750 mg/mL concentration of p-toluenesulfonyl-β-cyclodextrin.It was established that the coatings thus obtained are stable within pH 2-7 range but decompose in alkaline medium. The stationary phase developed in this study allows simultaneous separation of hydrophobic and hydrophilic analytes. Chiral separation of ketoprofen enantiomers was also achieved with the newly developed covalent coating.It was found that covalent coating based on imidazole and β-cyclodextrin exceeded the dynamic coatings of similar structure in terms of repeatability, analysis time and the limits of detection.

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Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

Cited by 28 publications

References 119 publications

3‐(4‐Carboxybenzoyl)quinoline‐2‐carboxaldehyde labeling for direct analysis of amino acids in plasma is not suitable for simultaneous quantification of tryptophan, tyrosine, valine, and isoleucine by CE/fluorescence

3‐(4‐Carboxybenzoyl)quinoline‐2‐carboxaldehyde labeling for direct analysis of amino acids in plasma is not suitable for simultaneous quantification of tryptophan, tyrosine, valine, and isoleucine by CE/fluorescence

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Multifunction covalent coatings for separation of amino acids, biogenic amines, steroid hormones, and ketoprofen enantiomers by capillary electrophoresis and capillary electrochromatography

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