Analytical capillary isotachophoresis after 50 years of development: Recent progress 2014–2016

Malá, Zdena; Gebauer, Petr; Boček, Petr

doi:10.1002/elps.201600289

Cited by 28 publications

(15 citation statements)

References 75 publications

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“…As shown in the recent reviews on capillary and microchip ITP , this CE method is much more often used for determination of small ions, e.g. counterions or ionic admixtures of peptides than to analyses of peptides themselves.…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…The usual procedure to achieve enantiomeric separation in techniques such as liquid chromatography (LC), gas chromatography or thinlayer chromatography, involves the interaction with a chiral compound or "selector" in stationary or mobile phase. Capillary electrophoresis (CE) is a liquid phase separation technique, which offers the alternative of being operated under several modes depending on the sample properties [1][2][3][4][5][6]. Since it does not necessary involve typical parabolic flow profiles of pressure driven techniques, higher efficiencies can be achieved.…”

Section: Highlightsmentioning

confidence: 99%

Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration

Lancioni

Keunchkarian

Castells

et al. 2018

Journal of Chromatography A

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A method to optimize the ligand concentration [S] in the background electrolyte of capillary electrophoresis separations is presented. It is based on the use of a model which predicts apparent electrophoretic mobilities as a function of ligand concentration (expressed as p[S] = -log[S]). This model is employed to compose the expression of a recently proposed criterion to qualify separations in electrophoresis. Two strategies to find the optimum p[S], leading to the best separation of all compounds, are explained: 1.- a graphical method using a windows map depicting the single separation criteria between all possible combination of compounds by pairs, and 2.- an analytical method where an extended multicriterion optimization function is composed and optimum p[S] is found by mathematical maximization. The procedure is applied to a hard-to-separate model system: enantiomeric separations of racemic mixtures. 2-Hydroxypropyl-β-cyclodextrin was chosen as a model ligand, and four pharmaceutical drugs as model analytes. In order to demonstrate the performance of the procedure, results of electrophoretic separations obtained at p[S] found as optimum are compared with separations obtained at p[S] values slightly higher and lower than the optimum.

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“…In both FASS and ITP, a buffer mismatch is used to manipulate an externally applied electric field to cause DNA to preferentially accumulate at the interface 57 , 58 . DNA analysis with FASS-CE, ITP-CE, and related electrokinetic platforms can boast limits of detection in the range of low pM to tens of fM 13 , 59 , 60 . In our technique, the electric field is generated internally via pressure-driven flow and diffusion, eliminating the need for an external input.…”

Section: Discussionmentioning

confidence: 99%

Molecular rheotaxis directs DNA migration and concentration against a pressure-driven flow

et al. 2017

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In-line preconcentration techniques are used to improve the sensitivity of microfluidic DNA analysis platforms. The most common methods are electrokinetic and require an externally applied electric field. Here we describe a microfluidic DNA preconcentration technique that does not require an external field. Instead, pressure-driven flow from a fluid-filled microcapillary into a lower ionic strength DNA sample reservoir induces spontaneous DNA migration against the direction of flow. This migratory phenomenon that we call Molecular Rheotaxis initiates in seconds and results in a concentrated DNA bolus at the capillary orifice. We demonstrate the ease with which this concentration method can be integrated into a microfluidic total analysis system composed of in-line DNA preconcentration, size separation, and single-molecule detection. Paired experimental and numerical simulation results are used to delineate the parameters required to induce Molecular Rheotaxis, elucidate the underlying mechanism, and optimize conditions to achieve DNA concentration factors exceeding 10,000 fold.

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Analytical capillary isotachophoresis after 50 years of development: Recent progress 2014–2016

Cited by 28 publications

References 75 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration

Molecular rheotaxis directs DNA migration and concentration against a pressure-driven flow

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