Precisão da tira reagente e do exame microscópico da urina no diagnóstico de infecções do trato urinário em porcas

Abstract

ABSTRACT.-Mazutti K., Locatelli-Dittrich R., Lunardon I., Kuchiishi S.S., Lara A.C., Zotti E. & Alberton G.C. 2013. Evaluation of the reagent test strips and microscopic examination of urine in the diagnosis of urinary tract infection in sows. Pesquisa Veterinária Brasileira 33(9):1103-1108. Curso de Medicina Veterinária, Escola de Ciências Agrárias e Medicina Veterinária, Pontifícia Universidade Católica do Paraná, Campus Curitiba, Rua Imaculada Conceição 1155, Prado Velho, Curitiba, PR 80215-901, Brazil. E-mail: kelly.mazutti@pucpr.brThe diagnosis of the urinary tract infection (UTI) in sows is usually performed by using reagent test strips, since it is a fast and practical method, and capable of being done at the farm. The microscopic examination of the urine is rarely used at the farm since it is a more time consuming and difficult technique. However, there are no studies on the accuracy of those two techniques for the UTI diagnosis on this species. This study aims to assess the accuracy of the reagent test strip and the urine microscopic examination in the diagnosis of ITU in sows, comparing them with the bacteriological examination of urine. In order to select the sows for this study, a chemical reagent test strip was carried out previously and a total of 139 sows were selected, 66 sows of which showed positivity to nitrite in the reagent test strip and 73 without nitrituria. Then, the next day, a new sample collection for performing a complete urinalysis was carried out from those 139 sows, which included physical, chemical, microscopic and microbiological examination of these urine samples. The results revealed that the nitrite test of the reagent strip showed 100% of specificity and 93% of sensitivity. The specificity of the microscopic examination for bacteriuria was 82% and the sensitivity was 100%. The UTI diagnosis by using reagent strips and/or the urine sediment test is reliable if compared to the urine bacteriological examination, which makes possible the rapid diagnosis of UTI in sows at the farm. RESUMO.-[Precisão da tira reagente e do exame microscópico da urina no diagnóstico de infecções do trato urinário em porcas.] O diagnóstico de infecção do trato urinário (ITU) em porcas geralmente é feito com o auxílio de tiras reagentes, por ser um método rápido, prático e passível de ser realizado na própria granja. O exame microscópico da urina raramente é utilizado em granjas por ser uma técnica mais demorada e trabalhosa. No entanto, não existem estudos sobre a precisão destas duas técni-cas no diagnóstico de ITU nesta espécie. O objetivo deste estudo foi avaliar a precisão da tira reagente e do exame microscópico da urina no diagnóstico de ITU em porcas, comparando-os com o exame bacteriológico da urina. Para selecionar as porcas que iriam compor o estudo foi realizado um exame químico prévio com tira reagente, do qual foram selecionadas 139 porcas, 66 positivas para nitrito na tira reagente e 73 negativas. No dia seguinte foi realizada uma nova coleta de urina destas 139 porcas para realização d...

Cystitis: significant associations between pathology, histology, and quantitative bacteriology in sows, a cross-sectional study

“…This study shows that 69% of the samples with bacteriuria harbored E. coli . This result correlates well with other studies conducted the last 20 years [ 13 , 17 , 25 , 26 ]. The fact that only 2 samples of E. coli had a CFU counts lower than 10 3 , implies that E. coli is a likely cause of cystitis in sows, as described both in humans and other animal species where UTI is a common disease condition [ 32 – 34 ].…”

Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection

“…2008; Merlini and Merlini 2011; Mazutti et al. 2013). The high frequency of E. coli in mixed infections should not be disregarded or merely considered as contamination, since over 80% of the studied E. coli isolates were characterized as multiresistant and 93% of them presented at least one virulence gene related to urinary tract infections, among foc H , pap C , sfa, afa, hly A , iuc D and cnf 1 genes (data not shown).…”

Correlation between urinary tract infection and reproductive performance of sows

“…Prevalence above 15% indicates a severe, evolving problem, while prevalence above 25% indicates a very severe, chronic problem. After the prevalence is known, the next step is to carry out the bacteriological test and antibiogram of samples with positive results in the reagent strip test to verify the etiological agents and define the best course of therapy (Sobestiansky et al, 1999;Mazutti et al, 2013).…”