Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection

Moreno, Luisa Zanolli; Matajira, Carlos Emílio Cabrera; Poor, André Pegoraro; Mesquita, Renan E.; Gomes, Vasco T.M.; Silva, Ana Paula S.; Amigo, Cristina Román; Christ, Ana Paula Guarnieri; Barbosa, Mikaela Renata Funada; Sato, Maria Inês Zanoli; Moreno, Andréa Micke

doi:10.1080/01652176.2017.1397302

Cited by 16 publications

(20 citation statements)

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“…and Gram-positive (Staphylococcus spp., Enterococcus spp.) isolates were resistant to the vast majority of antibiotics considered, a nding in line with previous relevant reports 15,37 . Based on our ndings, the narrow range of options for antimicrobial treatment of urinary tract infections in the studied herds only includes cephalosporins or polymyxins; both last-resort categories of drugs against multi-resistant bacteria strains of major signi cance in human health 38 .…”

Section: Discussionsupporting

confidence: 90%

Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis

Cernat

Skampardonis

Papadopoulos

et al. 2020

Preprint

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Background Urinary tract infections (UTI) of sows which include cystitis, which may progress to ureteritis and pyelonephritis affect their productivity, longevity and welfare. In this study we determined the prevalence of UTI by histopathology and bacteriology and investigated possible associations between histologically confirmed cystitis and the results of urinalysis and urine cultures in culled sows from three Greek farrow-to-finish herds. Materials and methods Routinely culled sows were included in the study. Their urinary bladders were collected from abattoirs and examined histopathologically. Furthermore, urinalysis and urine cultures were performed on urine samples aseptically collected from the bladders. Results Histologically confirmed cystitis was evident in 85/185 (45.94%) culled sows. Among those, 44 (51.76%) suffered from acute and 41 (48.24%) from chronic inflammation. The majority of the positive urine cultures were due to colonization of the urinary tract with E.coli, which was responsible for 55.81% of the total cases, followed by Staphylococcus spp. which caused 18.60% of the infections detected. Evidence of cystitis was associated with bacteriuria and sows with bacteriuria were 2.30 (p = 0.03, 95% CI: 1.10–4.83) times more likely to have histologically confirmed cystitis compared to sows with negative urine cultures. Bacteriuria was associated with proteinuria (p < 0.01), urine pH (p < 0.01) and presence of sediment (p < 0.01) in urine. Sows with proteinuria had 9.72 (2.63–35.88) times higher odds of bacteriuria than those without. Histologically defined cystitis was associated with proteinuria (p < 0.01) and increased urine pH (p < 0.01). Sows with proteinuria were 5.18 times (2.03–13.2) more likely to have histological lesions consistent with cystitis, than those without. Conclusions In the studied herds, UTI affected almost one out of two culled sows. Bacteriuria, which was more common among sows with UTI than those without, was mainly ascribed to members of the intestinal and environmental microbiota. Proteinuria and the existence of urine sediment which were associated with UTI may be proposed as likely on-farm predictors of UTI in live sows.

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Section: Discussionsupporting

confidence: 90%

Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis

Cernat

Skampardonis

Papadopoulos

et al. 2020

Preprint

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“…These bacteria which likely originate from sows’ intestinal tract, contaminate the environment of pig houses and ascend and colonize the lower part of the urinary tract [ 12 , 40 , 41 ]. Interestingly, we found only 2 sows infected with Actinobaculum suis , a specific contaminant of the urinary tract with descending significance compared to data reported in the past mainly due the wide application of artificial insemination [ 12 , 42 ].…”

Section: Discussionmentioning

confidence: 52%

Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis

et al. 2021

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Background Urinary tract infections (UTI) of sows characterized by cystitis, which may progress to ureteritis and pyelonephritis, can affect their productivity, longevity and welfare. In this study, we determined the prevalence of UTI by histopathology and bacteriology. Furthermore, we investigated possible associations between histologically confirmed cystitis and the results of urinalysis and urine cultures in culled sows from three farrow-to-finish herds in Greece. Materials and methods One hundred eighty-five routinely culled sows were included in the study. Their urinary bladder was collected from abattoirs and subjected to histopathology. Furthermore, urinalysis and urine cultures were performed on urine samples aseptically collected from the bladders. Results Histologically confirmed cystitis was evident in 85/185 (45.94%) culled sows. Among those, 44 (51.76%) suffered from acute and 41 (48.24%) from chronic inflammation. The majority of positive urine cultures were due to colonization of the urinary tract with E.coli, which accounted for 55.81% of the total cases, followed by Staphylococcus spp. which accounted for 18.60% of detected infections. Evidence of cystitis was associated with bacteriuria and sows with bacteriuria were 2.30 (P = 0.03, 95% CI: 1.10–4.83) times more likely to have histologically confirmed cystitis compared to sows with negative urine cultures. Bacteriuria was associated with proteinuria (P < 0.01, OR = 9.72, 2.63–35.88), increased urine pH (P < 0.01, 3.40, 1.10–10.56) and presence of sediment (P < 0.01, 6.00, 1.50–23.76) in urine. Sows with proteinuria had 9.72 (P < 0.01, 2.63–35.88) times higher odds of bacteriuria than those without. Histologically defined cystitis was associated with proteinuria (P < 0.01, 2.03–13.20) and decreased urine pH (P < 0.01, 0.13–0.72). Sows with proteinuria were 5.18 (P < 0.01, 2.03–13.2) times more likely to have histological lesions consistent with cystitis, than those without. For one unit increase in pH, it was 3.20 (P = 0.006, 1.39–7.69) times less likely for a sow to have chronic or acute cystitis compared to absence of cystitis. Conclusion In the studied population, UTI affected almost one out of two culled sows. Bacteriuria, which was more common among sows with UTI than those without, was mainly ascribed to members of the intestinal and environmental bacteria. Proteinuria and the existence of urine sediment which were associated with UTI, could be used as proxy traits for UTI in live sows.

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“…Molecular studies such as Protein Chain Reaction (PCR) are not widely available for M. marinum . The cultures should be incubated at 32 °C [ 5 ].…”

Section: Infections By Mycobacterium Marinum Anmentioning

confidence: 99%

“…The response is typically slow. The therapy is often maintained for 4 to 6 weeks after the lesions resolve [ 5 ].…”

Section: Infections By Mycobacterium Marinum Anmentioning

confidence: 99%

Nodular Lymphangitis (Sporotrichoid Lymphocutaneous Infections). Clues to Differential Diagnosis

Tirado‐Sánchez

Bonifáz

2018

JoF

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Nodular lymphangitis, also known as sporotrichoid lymphocutaneous infections, is characterized by suppurative inflammatory nodules along the lymphatic vessels. This manifestation is classic of sporotrichosis, however, other infections such as nocardiosis, atypical mycobacteriosis, leishmaniasis, among others, can also express this clinical pattern. Sporotrichosis, which often occurs in gardeners, remains the most recognized cause of nodular lymphangitis. The histopathological studies, as well as the culture are diagnostic standards of lesions that do not respond to empirical treatment. In this article, we will review the main causes of nodular lymphangitis or lymphocutaneous sporotrichoid infections.

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Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection

Abstract: MALDI-TOF MS enabled the identification of rare pathogens related to UTI which may represent higher risk for porcine health, especially considering high frequency of multiresistant profiles.

Cited by 16 publications

References 13 publications

Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis

Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis

Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis

Nodular Lymphangitis (Sporotrichoid Lymphocutaneous Infections). Clues to Differential Diagnosis

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