Precipitation methods for the determination of LDL-cholesterol

Kerscher, Lorenz; Schiefer, Sigbert; Draeger, Brigitte; Maier, Josef; Ziegenhorn, Joachim

doi:10.1016/s0009-9120(85)80093-x

Cited by 27 publications

(19 citation statements)

References 19 publications

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“…Blood glucose and triglycerides, total cholesterol, low-density lipoprotein cholesterol (ldl-c) and high-density lipoprotein cholesterol (Hdl-c) (n=10) were estimated using commercial kits (BioSino Bio-Technology and Science inc., Beijing, china) (8)(9)(10)(11)(12). Fecal lipid assays were conducted according to the following protocol.…”

Section: Animals and Dietsmentioning

confidence: 99%

Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice

et al. 2010

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Abstract. certain individuals ingesting a low-fat diet are still prone to visceral obesity, and the reasons remain undetermined. This study aimed to investigate differentially expressed proteins of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone c57Bl/6 mice through a proteomics approach and to ascertain potential mechanisms associated with these obesity-prone animals. First, animals were fed low-fat or normal-chow diets for 6 weeks. dietary intake and body weight were monitored during this period. after 6 weeks of the low-fat diet, obesityprone and obesity-resistant mice (noP and nor groups) were defined as those within the upper and lower quarters for weight gain, respectively. Proteomic analysis showed that ubiquinol-cytochrome c reductase core protein 1 (Uqcrc1) and enolase 3, β muscle were decreased by 8.1-and 8.8-fold in the visceral adipose tissues of the noP mice compared to the nor controls, respectively, while monoglyceride lipase (MGll) and glucose-6-phosphate dehydrogenase (G6PdH) X-linked were increased by 5.3-and 4.7-fold, respectively. These results indicate that obesity-prone animals fed low-fat diets exhibited differentially expressed proteins, e.g., Uqcrc1, enolase 3, MGll and G6PdH involved in energy metabolism, glycolysis and fat synthesis in visceral adipose tissues, which are useful for defining molecular markers and understanding the mechanisms of the development of obesity.

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Section: Animals and Dietsmentioning

confidence: 99%

Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice

et al. 2010

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“…The HMG CoA reductase catalyses the rate limiting step in cholesterol synthesis 10 . The activity of HMG CoA reductase is regulated by the nutritional and hormonal state of animals.…”

Section: Discussionmentioning

confidence: 99%

Possible inhibition of hydroxy methyl glutaryl CoA reductase activity by nicotinic acid and ergosterol: as targeting for hypocholesterolemic action

et al. 2016

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Objective: Coronary artery diseases including atherosclerosis is considered as commonest problem worldwide. Ergosterols are the main components of vegetable oils and nuts. The objective of this study was to evaluate the potential hypoplipidemic and hypocholesterolemic effects of ergosterol in combination with niacin in rats fed high fat diet (HFD). Methods: Eighty male albino rats were included in this study divided into two main groups: Group I: Normal rats fed standard diet treated with either niacin (8.5 mg /kg b.w) or ergosterol (100 mg/Kg b.w) or both. Group II; rats fed HFD treated with either niacin (8.5 mg /kg b.w) or ergosterol (100 mg/Kg b.w) or both The feeding and treatment lasted for 8 weeks.Results: A significant elevation in the levels of total cholesterol, triacylglycerol, VLDL-c, LDL-c and atherogenic factor (p<0.001) in rats fed on HFD compared with normal control while HDL-c was significantly reduced in HFD rats compared with control group. Supplementation of diet with niacin or ergosterol or combined exerts improvement in the studied parameters by lowering triacylglycerol, total cholesterol, LDL-c and atherogenic factor and elevate HDL-c near to the value of control. Niacin combined with ergosterol were effective in the reduction of hydroxy methyl glutaryl-CoA reducatase (HMGCoA) compared with control (p<0.001). The combined effect was more potent than individual alone. Conclusion: Utilization of niacin and ergosterol may prevent the hypercholesterolemia and incidence of coronary heart diseases. These functional foods act as nutriceutical as dyslipidemics.

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“…Blood glucose and triglycerides, total cholesterol, low-density lipoprotein cholesterol and highdensity lipoprotein cholesterol (n=10) were estimated using commercial kits (BioSino Bio-technology and Science inc., Beijing, china) (10)(11)(12)(13)(14). Blood leptin levels were assayed through the avidin-biotin complex-EliSa kit (Shanghai Westang Bio-tech, china).…”

Section: Methodsmentioning

confidence: 99%

Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice

Xie

Wang

Zhang

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. this study aimed to investigate the potential mechanisms of natural resistance to high-fat diet-induced obesity. Four-week-old c57Bl/6 mice were fed a high-fat diet for 6 weeks and were then designated as high-fat diet-fed obesity-prone (hOp) and obesity-resistant (hOr) animals. their blood biochemistry was evaluated, and visceral adipose tissue samples were subjected to proteomic, Western blot and quantitative real-time pcr (q-pcr) analyses. the hOr mice showed reduced visceral fat weight and size, as well as lowered serum lipid and leptin levels. proteomic analysis showed that enoyl coenzyme a hydratase 1, peroxisomal (Ech1) expression was significantly increased in their visceral adipose tissues. moreover, other proteins, such as α-tropomyosin, myosin light chain, urine-nucleoside phosphorylase and transgelin, were also significantly increased. Furthermore, q-PCR analysis showed that the expression of acyl-coa oxidase 1 palmitoyl, enoyl-coa hydratase/3-hydroxyacyl-coa dehydrogenase and 3-oxoacyl-coa thiolase responsible for peroxisomal β-oxidation was also up-regulated in the visceral adipose tissues of the hOr mice. the expression of peroxisome proliferator-activated receptor α (pparα) was increased in the hOr mice as shown by Western blot analysis. Obesityresistant animals show enhanced peroxisomal β-oxidation metabolism and reduced fat accumulation in visceral adipose tissues by up-regulating the expression of Ech1, peroxisomal or other related peroxisomal β-oxidation marker genes, which may be driven or enhanced by the up-regulation of the expression of pparα. however, further validation in future studies is required.

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Precipitation methods for the determination of LDL-cholesterol

Cited by 27 publications

References 19 publications

Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice

Proteomic profile of visceral adipose tissues between low-fat diet-fed obesity-resistant and obesity-prone C57BL/6 mice

Possible inhibition of hydroxy methyl glutaryl CoA reductase activity by nicotinic acid and ergosterol: as targeting for hypocholesterolemic action

Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice

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