Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice

Abstract

Abstract. this study aimed to investigate the potential mechanisms of natural resistance to high-fat diet-induced obesity. Four-week-old c57Bl/6 mice were fed a high-fat diet for 6 weeks and were then designated as high-fat diet-fed obesity-prone (hOp) and obesity-resistant (hOr) animals. their blood biochemistry was evaluated, and visceral adipose tissue samples were subjected to proteomic, Western blot and quantitative real-time pcr (q-pcr) analyses. the hOr mice showed reduced visceral fat weight and size, as well as lowered serum lipid and leptin levels. proteomic analysis showed that enoyl coenzyme a hydratase 1, peroxisomal (Ech1) expression was significantly increased in their visceral adipose tissues. moreover, other proteins, such as α-tropomyosin, myosin light chain, urine-nucleoside phosphorylase and transgelin, were also significantly increased. Furthermore, q-PCR analysis showed that the expression of acyl-coa oxidase 1 palmitoyl, enoyl-coa hydratase/3-hydroxyacyl-coa dehydrogenase and 3-oxoacyl-coa thiolase responsible for peroxisomal β-oxidation was also up-regulated in the visceral adipose tissues of the hOr mice. the expression of peroxisome proliferator-activated receptor α (pparα) was increased in the hOr mice as shown by Western blot analysis. Obesityresistant animals show enhanced peroxisomal β-oxidation metabolism and reduced fat accumulation in visceral adipose tissues by up-regulating the expression of Ech1, peroxisomal or other related peroxisomal β-oxidation marker genes, which may be driven or enhanced by the up-regulation of the expression of pparα. however, further validation in future studies is required.

Icariin Is A PPARα Activator Inducing Lipid Metabolic Gene Expression in Mice

“…Peroxisomal FA β-oxidation is another important route of FA metabolism and is regulated by PPARα. Acox1, Ech1, and Ehhadh are key enzymes involved in peroxisomal β-oxidation [39,40,41,42]. Green tea polyphenols [40] and Compound K [42] alleviate obesity by induction of Acox1.…”

“…Green tea polyphenols [40] and Compound K [42] alleviate obesity by induction of Acox1. Ech1 is an important component of enhanced peroxisomal β-oxidation in C57 mice [39]. Ehhadh is indispensable for the production of medium-chain dicarboxylic acids, providing an explanation for the coordinated induction of mitochondrial and peroxisomal oxidative pathways during fasting [41].…”

Transcriptomic Responses in the Livers and Jejunal Mucosa of Pigs under Different Feeding Frequencies

“…Acetyl-CoA acyltransferase (ACAA), also known as 3-Ketoacyl-CoA thiolase, includes two subtypes, ACAA1 and ACAA2, which catalyze the last step of fatty acid beta oxidation. Previous studies have shown that the increase of ACAA activity promotes the oxidation of fatty acids [39,40]. The ACAA1 gene is mainly involved in physiological and biochemical processes such as the oxidation of very long-chain fatty acids, bile acid metabolism, and regulation of peroxisome proliferation [41].…”

New Role of Hispidulin in Lipid Metabolism: PPARα Activator

“…Consistently, our results here also indicate that enhancement of mitochondrial FA β-oxidation contributes to hispidulin induced hypolipidemic effects. Acox1, Ech1 and EHHADH are key enzymes in peroxisome FA oxidation, another crucial route of FA metabolism in mammalian animals [35]. Additionally, it has been reported that green tea polyphenols can modulate lipid metabolism by targeting peroxisome FA oxidation [36].…”