Pramipexole and Lactoferrin ameliorate Cyclophosphamide-Induced haemorrhagic cystitis via targeting Sphk1/S1P/MAPK, TLR-4/NF-κB, and NLRP3/caspase-1/IL-1β signalling pathways and modulating the Nrf2/HO-1 pathway

Baky, Nayira A. Abdel; Al-Najjar, Aya H.; Elariny, Hemat A.; Sallam, Amany Said; Mohammed, Asmaa N.

doi:10.1016/j.intimp.2022.109282

Cited by 10 publications

(5 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect is achieved through the inhibition of NLRP3 inflammasome activation and regulation of the NF-κB signaling pathway, both of which are closely linked to toll-like receptors (TLRs). Additionally, GA promotes the upregulation of nuclear factor E2-related factor 2 (Nrf2) and concurrently lowers the production of reactive oxygen species (ROS). ,,− In addition, immunofluorescence detection using corresponding antibodies to label macrophages with M1 pro-inflammatory phenotype or M2 anti-inflammatory phenotype is conducive to exploring the inflammatory state . Although a decrease in M1 phenotype and an increase in M2 phenotype have been observed in numerous anti-inflammatory studies involving GA, recent studies suggest that GA may be involved in inhibition of M2 phenotype to alleviate tissue fibrosis. − The anti-inflammatory effects of GA-PEPE are likely due to its antioxidant polyphenol structure and its targeting of the TLR anti-inflammatory pathway.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, GA promotes the upregulation of nuclear factor E2-related factor 2 (Nrf2) and concurrently lowers the production of reactive oxygen species (ROS). 50 , 77 , 85 − 87 In addition, immunofluorescence detection using corresponding antibodies to label macrophages with M1 pro-inflammatory phenotype or M2 anti-inflammatory phenotype is conducive to exploring the inflammatory state. 88 Although a decrease in M1 phenotype and an increase in M2 phenotype have been observed in numerous anti-inflammatory studies involving GA, recent studies suggest that GA may be involved in inhibition of M2 phenotype to alleviate tissue fibrosis.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Gallic Acid-Modified Polyethylenimine–Polypropylene Carbonate–Polyethylenimine Nanoparticles: Synthesis, Characterization, and Anti-Periodontitis Evaluation

Xie,

Gao,

Liu

et al. 2024

ACS Omega

View full text Add to dashboard Cite

The aim of the research was to develop novel gallic acid (GA)-modified amphiphilic nanoparticles of polyethylenimine (PEI)−polypropylene carbonate (PPC)−PEI (PEPE) and comprehensively assess its properties as an antiperiodontitis nanoparticle targeting the Toll-like receptor (TLR). The first step is to evaluate the binding potential of GA to the core trigger receptors TLR2 and TLR4/MD2 for periodontitis using molecular docking techniques. Following this, we conducted NMR, transmission electron microscopy, and dynamic light scattering analyses on the synthesized PEPE nanoparticles. As the final step, we investigated the synthetic results and in vitro antiperiodontitis properties of GA-PEPE nanoparticles. The investigation revealed that GA exhibits potential for targeted binding to TLR2 and the TLR4/MD2 complex. Furthermore, we successfully developed 91.19 nm positively charged PEPE nanoparticles. Spectroscopic analysis indicated the successful synthesis of GA-modified PEPE. Additionally, CCK8 results demonstrated that GA modification significantly reduced the biotoxicity of PEPE. The in vitro antiperiodontitis properties assessment illustrated that 6.25 μM of GA-PEPE nanoparticles significantly reduced the expression of pro-inflammatory factors TNF-α, IL-1β, and IL-6. The GA-PEPE nanoparticles, with their targeted TLR binding capabilities, were found to possess excellent biocompatibility and antiperiodontitis properties. GA-PEPE nanoparticles will provide highly innovative input into the development of anti-periodontitis nanoparticles.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Gallic Acid-Modified Polyethylenimine–Polypropylene Carbonate–Polyethylenimine Nanoparticles: Synthesis, Characterization, and Anti-Periodontitis Evaluation

Xie,

Gao,

Liu

et al. 2024

ACS Omega

View full text Add to dashboard Cite

show abstract

“…55 We have previously reported a close correlation between the induction of ROS-derived liver lipoperoxidation and the appearance of preneoplastic lesions. 38 On the other hand, the antioxidant potential of bLf has been widely documented; 52,56,57 however, it has been mostly attributed to the iron sequestering capability of native- or apo-bLf, since iron is a well-known pro-oxidant that induces ROS production through the Fenton reaction. 58 Therefore, we verified that iron-saturated bLf did not increase ROS production beyond the increase produced by DEN.…”

Section: Discussionmentioning

confidence: 99%

Iron-saturated bovine lactoferrin: a promising chemopreventive agent for hepatocellular carcinoma

Hernández-Galdámez,

Fattel-Fazenda,

Flores-Téllez

et al. 2024

Food Funct.

View full text Add to dashboard Cite

show abstract

“…The SphK1/S1P/NF-κB signaling pathway is implicated in the regulation of inflammation in different diseases, such as spinal cord injury, acute liver failure, and haemorrhagic cystitis. [38][39][40] Inactivation of SphK1/S1P/NF-κB pathway by diacerein leads to suppression of apoptosis and oxidative stress in an LPS-induced rat model of acute lung injury. 41 It is worth noting that resveratrol inhibited LPS-mediated inflammation and fibronectin expression in rat glomerular mesangial cells by blocking the SphK1/S1P/NF-κB pathway.…”

Section: Discussionmentioning

confidence: 99%

Baicalin Exerts a Protective Effect in Diabetic Nephropathy by Repressing Inflammation and Oxidative Stress Through the SphK1/S1P/NF-κB Signaling Pathway

Ren¹,

Jiao²,

Yan³

et al. 2023

DMSO

View full text Add to dashboard Cite

Background: Inflammation and oxidative stress contribute to the development of diabetic nephropathy (DN). Baicalin (BA) shows renal protection against DN through its anti-inflammatory and anti-oxidant properties. However, the molecular mechanism by which BA exerts the therapeutic effects on DN remains to be investigated. Methods: The db/db mice and high glucose (HG)-induced HK-2 cells were used as the in vivo and in vitro model of DN, respectively. The effects of BA were assessed by detecting the related blood and urine biochemical parameters, kidney histopathology, inflammatory cytokine production, oxidative stress indicators, and apoptosis. Cell viability and apoptosis were detected by CCK-8 assay and TUNEL assay, respectively. Related protein levels were measured by an immunoblotting method. Results: In db/db model mice, BA reduced serum glucose concentration, decreased blood lipid levels, ameliorated kidney functions, and decreased histopathological changes in kidney tissues. BA also alleviated oxidative stress and inflammation in db/db mice. In addition, BA blocked the activation of sphingosine kinases type 1/sphingosine 1-phosphate (SphK1/S1P)/NF-κB pathway in db/db mice. In HK-2 cells, BA hindered HG-induced apoptosis, oxidative stress and inflammation, while overexpression of SphK1 or S1P could reverse these effects. BA alleviated HG-induced apoptosis, oxidative stress and inflammation in HK-2 cells through the S1P/NF-κB pathway. Furthermore, BA blocked the NF-κB signaling by diminishing p65 nuclear translocation via the SphK1/S1P pathway. Conclusion: Our study strongly suggests that BA protects against DN via ameliorating inflammation, oxidative stress and apoptosis through the SphK1/S1P/NF-κB pathway. This study provides a novel insight into the therapeutic effects of BA in DN.

show abstract

Pramipexole and Lactoferrin ameliorate Cyclophosphamide-Induced haemorrhagic cystitis via targeting Sphk1/S1P/MAPK, TLR-4/NF-κB, and NLRP3/caspase-1/IL-1β signalling pathways and modulating the Nrf2/HO-1 pathway

Cited by 10 publications

References 106 publications

Gallic Acid-Modified Polyethylenimine–Polypropylene Carbonate–Polyethylenimine Nanoparticles: Synthesis, Characterization, and Anti-Periodontitis Evaluation

Gallic Acid-Modified Polyethylenimine–Polypropylene Carbonate–Polyethylenimine Nanoparticles: Synthesis, Characterization, and Anti-Periodontitis Evaluation

Iron-saturated bovine lactoferrin: a promising chemopreventive agent for hepatocellular carcinoma

Baicalin Exerts a Protective Effect in Diabetic Nephropathy by Repressing Inflammation and Oxidative Stress Through the SphK1/S1P/NF-κB Signaling Pathway

Contact Info

Product

Resources

About