Potential roles of two Cathepsin genes, DcCath-L and DcCath-O in the innate immune response of Diaphorina citri

Yu, Hai‐Zhong; Huang, Yu‐Ling; Li, Ning‐Yan; Xie, Yan-Xin; Zhou, Chenghua; Lu, Zhanjun

doi:10.1016/j.aspen.2019.05.010

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…1B). Moreover, cathepsin genes are up-regulated in response to Las (Ramsey et al 2017;Yu et al 2019). Cathepsins are multifunctional proteins involved in apoptosis, immune response and development (Sun et al 2017).…”

Section: Immune System and Metabolismmentioning

confidence: 99%

Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri

Galdeano¹,

Pacheco²,

Alves

et al. 2020

Trop. plant pathol.

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Interactions between insects and plant pathogens have been more enthusiastically studied in the recent decade, especially those relationships which takes the insects as vectors. The spectrum of these interactions ranges from mutualistic to pathogenic. The length of the co-evolutionary process will determine whether a microorganism shares a friend or a foe relationship with its host, and a friendship connection is frequently observed if the coexistence is longer. This review updates knowledge about the morphological, physiological and genetic mechanisms that drive the interaction between 'Candidatus Liberibacter asiaticus' (Las) and its vector, the Asian citrus psyllid, Diaphorina citri. Las is the predominant causal agent of citrus huanglongbing (HLB) disease, the major constrain to citrus production worldwide. This bacterium is transmitted by D. citri, in a propagative-circulative manner during its feeding from plant host. Understanding of the interactions among vector, plant pathogen and host plant are important for the management of this vector-borne disease complex.

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Section: Immune System and Metabolismmentioning

confidence: 99%

Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri

Galdeano¹,

Pacheco²,

Alves

et al. 2020

Trop. plant pathol.

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“…Bacterial preparation and treatment were performed as previously described [31]. Briefly, the overnight cultured E. coli (gram-negative) and S. aureus (gram-positive) cells were harvested and centrifuged at 7500× g for 15 min.…”

Section: Bacterial Challenge and Sample Preparationmentioning

confidence: 99%

Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri

et al. 2019

IJMS

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Chitin deacetylase (CDA) is a chitin degradation enzyme that strictly catalyzes the deacetylation of chitin to form chitosan, which plays an important role in regulating growth and development, as well as the immune response. In this study, a chitin deacetylase 3 gene (CDA3) was identified with a complete open reading frame (ORF) of 1362 bp from the genome database of Diaphorina citri, encoding a protein of 453 amino acids. Spatiotemporal expression analysis suggested that D. citri CDA3 (DcCDA3) had the highest expression level in the integument and third-instar nymph stage. Furthermore, DcCDA3 expression level can be induced by 20-hydroxyecdysone (20E). Injection of Escherichia coli and Staphylococcus aureus induced the upregulation of DcCDA3 in the midgut, while DcCDA3 was downregulated in the fat body. After silencing DcCDA3 by RNA interference, there was no influence on the D. citri phenotype. In addition, bactericidal tests showed that recombinant DcCDA3 inhibited gram-positive bacteria, including S. aureus and Bacillus subtilis (B. subtilis). In conclusion, our results suggest that DcCDA3 might play an important role in the immune response of D. citri.

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“…The cathepsins are involved in processes like development, growth, metamorphosis, apoptosis and immunity (Saikhedkar et al, 2015). For example, two D. citri cathepsin genes were significantly induced by CLas, suggesting that these genes play an important role in the immune response (Yu et al, 2019). In addition, cathepsin is a cysteine protease and functions as a primary digestive enzyme to degrade host defense proteins.…”

Section: Discussionmentioning

confidence: 99%

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

et al. 2020

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The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the principal vector of the Candidatus Liberibacter asiaticus (CLas) bacterium that causes Huanglongbing (HLB) disease. The D. citri salivary glands (SG) is an important barrier to the transmission of CLas. Despite its importance, the transcriptome and proteome of SG defense against CLas are unstudied in D. citri. In the present study, we generated a comparative transcriptome dataset of the SG in infected and uninfected D. citri using an Illumina RNA-Seq technology. We obtained 407 differentially expressed genes (DEGs), including 159 upregulated DEGs and 248 downregulated DEGs. Functional categories showed that many DEGs were associated with the ribosome, the insecticide resistance, the immune response and the digestion in comparison with CLas-infected SG and CLasfree SG. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases confirmed that metabolism and immunity were important functions in the SG. Among the DEGs, 68 genes (35 upregulated and 33 downregulated) encoding putativesecreted proteins were obtained with a signal peptide, suggesting that these genes may play important roles in CLas infection. A total of 673 SG proteins were identified in uninfected D. citri by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis, and 30 DEGs (15 upregulated and 15 downregulated) were found using the local tBLASTP programs. Among the 30 DEGs, many DEGs mainly involved in the metabolism and cellular processes pathways. This study provides basic transcriptome and proteome information for the SG in D. citri, and helps illuminate the molecular interactions between CLas and D. citri.

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Potential roles of two Cathepsin genes, DcCath-L and DcCath-O in the innate immune response of Diaphorina citri

Cited by 9 publications

References 52 publications

Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri

Friend or foe? Relationship between ‘Candidatus Liberibacter asiaticus’ and Diaphorina citri

Immune Functional Analysis of Chitin Deacetylase 3 from the Asian Citrus Psyllid Diaphorina citri

A Transcriptomic and Proteomic Analysis of the Diaphorina citri Salivary Glands Reveals Genes Responding to Candidatus Liberibacter asiaticus

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