“…Sequence-specific dsRNA injection was demonstrated to be the most successful strategy for gene-specific RNA knockdown in most decapod crustaceans, and provides an alternative means of increasing knowledge about their physiology ( Sagi, Manor & Ventura , 2013 ). Although RNAi technique has been applied to understand various aspects of physiology, including growth and development ( De Santis et al , 2011 ; Glazer et al , 2010 ; Soñanez-Organis, Racotta & Yepiz-Plascencia , 2010 ), immunity ( Robalino et al , 2007 ), and reproduction ( Nagaraju, Rajitha & Borst , 2011 ; Sathapondecha et al , 2011 ; Treerattrakool, Panyim & Udomkit , 2011 ), the cellular mechanism of this method is still not clearly understood in decapod crustaceans, and the doses, types and sizes of RNA vary for each gene examined. In the present study, transcription of Liv-AdipoR was suppressed by up to 95% in thoracic muscle and deep abdominal muscle, whereas its suppressive effects in the hepatopancreas differed according to the dsRNA concentration injected ( Fig.…”