Potential roles of transglutaminase and thioredoxin in the release of gonad-stimulating factor in Penaeus monodon: Implication from differential expression in the brain during ovarian maturation cycle

Gene Silencing in Crustaceans: From Basic Research to Biotechnologies

“…However, expression levels of GIH in the shrimp fed with GIH dsRNA (72%) in comparison with dsRNA injection (32%) showed that oral administration was significantly less effective. Sathapondecha et al [ 51 ] studied the effect of ds-GIH on the expression of other genes in P. monodon . Among the genes that were found to be up regulated in the ds-GIH injected females were thioredoxin and transglutaminase .…”

“…Among the genes that were found to be up regulated in the ds-GIH injected females were thioredoxin and transglutaminase . The expression of the both latter genes was strongly up-regulated in the brains of early-vitellogenic ds-GIH treated female shrimp, suggesting that thioredoxin and transglutaminase are both required for an initial stage of vitellogenesis [ 51 ].…”

Characterization, expression, and function analysis of gonad-inhibiting hormone in Oriental River prawn, Macrobrachium nipponense and its induced expression by temperature

“…In decapod crustaceans, GIH was first isolated and characterized in the eyestalk extracts of Homarus americanus (Soyez et al, 1987) and then was studied in other a few species such as Macrobrachium rosenbergii, Nephrops norvegicus, Penaeus monodon, and Litopenaeus vannamei (Yang and Rao, 2001;Edomi et al, 2002;Ohira et al, 2006;Treerattrakool et al, 2008;Hopkins, 2012;Chen et al, 2014). More recently, GIH was silenced by GIH dsRNA injections in P. monodon (Treerattrakool et al, 2008Sathapondecha et al, 2011;Sagi et al, 2013;Treerattrakool et al, 2013). However, there is a lack of information regarding GIH gene in M. nipponense.…”

Molecular characterization of an adiponectin receptor homolog in the white leg shrimp,Litopenaeus vannamei

“…Sequence-specific dsRNA injection was demonstrated to be the most successful strategy for gene-specific RNA knockdown in most decapod crustaceans, and provides an alternative means of increasing knowledge about their physiology ( Sagi, Manor & Ventura , 2013 ). Although RNAi technique has been applied to understand various aspects of physiology, including growth and development ( De Santis et al , 2011 ; Glazer et al , 2010 ; Soñanez-Organis, Racotta & Yepiz-Plascencia , 2010 ), immunity ( Robalino et al , 2007 ), and reproduction ( Nagaraju, Rajitha & Borst , 2011 ; Sathapondecha et al , 2011 ; Treerattrakool, Panyim & Udomkit , 2011 ), the cellular mechanism of this method is still not clearly understood in decapod crustaceans, and the doses, types and sizes of RNA vary for each gene examined. In the present study, transcription of Liv-AdipoR was suppressed by up to 95% in thoracic muscle and deep abdominal muscle, whereas its suppressive effects in the hepatopancreas differed according to the dsRNA concentration injected ( Fig.…”

“…The recent advancements of next generation sequencing (NGS) technology and RNA interference (RNAi) technique have enabled researchers who study the non-model organisms, including decapod crustaceans, to expand their knowledge by using those techniques with a limited budget. For example, transcriptional suppression of target gene can be achieved simply by injecting long dsRNA, and this method has changed the strategy for studying crustacean physiology, including growth and development ( De Santis et al , 2011 ; Glazer et al , 2010 ; Lee et al , 2015 ; Soñanez Organis, Racotta & Yepiz-Plascencia , 2010 ), immunity ( Robalino et al , 2007 ), and reproduction ( Nagaraju, Rajitha & Borst , 2011 ; Sathapondecha et al , 2011 ; Treerattrakool, Panyim & Udomkit , 2011 ).…”