Characterization, expression, and function analysis of gonad-inhibiting hormone in Oriental River prawn, Macrobrachium nipponense and its induced expression by temperature

Qiao, Hui; Xiong, Yiwei; Zhang, Wenyi; Fu, Hongtuo; Jiang, Sufei; Sun, Shengming; Bai, Hongkun; Jin, Shubo; Gong, Yongsheng

doi:10.1016/j.cbpa.2015.03.005

Cited by 97 publications

(79 citation statements)

References 49 publications

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“…The relative expression level of PcC1q-like mRNA significantly decreased by~82% (Fig. 7) one day after 24 h of the second injection, which is comparable to the RNAi efficiencies in the prawn M. nipponense for knockdown of the GIH gene (Qiao et al, 2015) and in the crab E. sinensis for knockdown of EsDmrt-like gene (Ma et al, 2016). This indicates an efficient interference of dsPcC1q-like in this study.…”

Section: Discussionsupporting

confidence: 61%

Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii

et al. 2017

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Section: Discussionsupporting

confidence: 61%

Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii

et al. 2017

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“…Higher future global temperature is unequivocal as IPCC predicts an increase in global average surface temperature from +2 to +7°C for the year 2100 depending on the scenario (IPCC, ). Hsp70 represents a molecular chaperone that is well known for its rapid responses to environmental stresses (Qiao et al., ). This study focused on the gene expression level of Hsp70 in the ovary and hepatopancreas of M. rosenbergii in response to different thermal stress conditions and how well their reproductive performance in that thermal stress circumstances.…”

Section: Discussionmentioning

confidence: 99%

“…Not only that, the Hsp70 expression in treatment with 2 hr recovery period at 28°C, T3 in similar organ demonstrated higher expression of Hsp70 at 6, 24 hr and 30 days compared to control and without recovery period. These showed that the induction of Hsp70 was time‐dependent, where the expression of Hsp70 depend on the duration of the prawns being exposed to the treatment (Qiao et al., ) and the duration of the organisms under recovery or normal temperature (Sung, Pineda, MacRae, Sorgeloos & Bossier, ). Environment temperature induced short‐ and long‐term (acclimatory) thermal stress responses on biochemical and physiological level to aid the organisms to survive in stressful condition.…”

Section: Discussionmentioning

confidence: 99%

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii

et al. 2017

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Using Hsp70 as a biomarker, thermal stress impinges on reproductive organs, ovary and hepatopancreas were being analyzed by determining the expression of Hsp70 mRNA inside the organs after the adult inter-molt females were subjected to thermal treatment at 35, 30 and 28°C (Control). Results showed the expression of Hsp70 mRNA under thermal treatment of 35°C after 2 hr recovery in ovary were upregulated at 2, 4, 6, 12, 24 hr and 30 days compared to control whereas in hepatopancreas under similar treatment, the expression of Hsp70 mRNA were significantly higher than control at 6, 24 hr and 30 days. Frequency of reproductive molt at 35°Cshowed the ovary of females were failed to develop and only entered common molt along three consecutive molt cycles. For 30°C thermal treatment, the expression of Hsp70 mRNA was significantly higher than control after 2 hr recovery but returned to normal afterwards until 30 days' thermal treatment. Maternal heat shock for 2 hr at 35°C were found to give significantly lower frequency of reproductive molt and longer duration of ovarian development and incubation period whereas maternal heat shock for 2 hr at 30°C gave lower frequency of reproductive molt, slower development of embryo and lower hatching success compared to untreated control. This study suggests that short and long-term thermal stress at 30 and 35°C were found to affect the induction of Hsp70 mRNA in reproductive organs of Macrobrachium rosenbergii and also influence their reproductive performance. K E Y W O R D Sheat shock protein 70, Macrobrachium rosenbergii, reproductive performance, thermal stress

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“…The developmental stage of the embryo was classified into seven stages based on the criteria of Qiao et al (2015) [41]. The ovarian cycle of the prawns was classified into five stages based on the previous results Gao et al, (2006) [42].…”

Section: Methodsmentioning

confidence: 99%

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

Qiao

et al. 2018

IJMS

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Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used. Recent studies have investigated suitable reference genes for some crustaceans under various conditions, but studies in Macrobrachium nipponense are currently lacking. In this study, we selected the following seven genes from among 35 commonly used housekeeping genes as candidate qPCR reference genes for temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S ribosomal RNA), EF-1α (elongation factor-1α), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (α-tubulin), β-act (β-actin), and RPL18 (Ribosomal protein L18). The stability of each reference gene was evaluated by GeNorm, NormFinder, BestKeeper, and comparative ∆C t methods, and was comprehensively ranked using RefFinder. RPL18 was shown to be the most suitable reference gene for adult M. nipponense tissues, while EIF was the most stable in different ovarian and embryo stages and in white spot syndrome virus infection, and β-act was the most stable reference gene under hypoxia stress. The reliability of the rankings was confirmed by RNA interference experiments. To the best of our knowledge, this represents the first systematic analysis of reference genes for qPCR experiments in M. nipponense, and the results will provide invaluable information for future research in closely related crustaceans.

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Characterization, expression, and function analysis of gonad-inhibiting hormone in Oriental River prawn, Macrobrachium nipponense and its induced expression by temperature

Cited by 97 publications

References 49 publications

Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii

Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

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