Molecular characterization of an adiponectin receptor homolog in the white leg shrimp,<i>Litopenaeus vannamei</i>

Kim, Ah Ran; Alam, Md. Jobaidul; Yoon, Tae-Ho; Lee, Soo Rin; Park, Hyun; Kim, Doo-Nam; An, Doo-hae; Lee, Jae-Bong; Lee, Chung Il; Kim, Hyun Woo

doi:10.7717/peerj.2221

Cited by 8 publications

(2 citation statements)

References 65 publications

(84 reference statements)

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“…There was also a similar effect on the expression of lipophorin, all of which suggest that the mosquito adiponectin receptor may be modulated by an incoming blood meal which contains mammalian adiponectin. Since different species of insects have homologues of adiponectin receptor proteins ( 40 ), it is possible that other ligands, such as C1q-like proteins, can activate the adiponectin receptor. While homology searches do not uncover any C1q-like protein in mosquitoes, we cannot exclude the possibility that there are additional unknown ligands for the adiponectin receptor in mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

Chuang,

Stone,

Abouneameh

et al. 2024

mBio

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When a female mosquito engorges on a mammalian host, components of the blood meal can affect mosquito fitness and indirectly influence pathogen infectivity. We demonstrate that mammalian adiponectin, ingested during Anopheles gambiae blood feeding, co-localizes within mosquito midguts and decreases Plasmodium infection in the vector. Transcriptomic and RNAi studies reveal that the A. gambiae adiponectin receptor is involved in downregulating lipophorin, a lipid transporter that is important for egg development and Plasmodium infection in mosquitoes. These studies characterize a cross-phyla interaction between the mammalian host and A. gambiae that negatively impacts Plasmodium survival in its arthropod vector. IMPORTANCE When a female mosquito takes a blood meal from a mammalian host, components of the blood meal can affect mosquito fitness and indirectly influence pathogen infectivity. We identified a pathway involving an Anopheles gambiae adiponectin receptor, which, triggered by adiponectin from an incoming blood meal, decreases Plasmodium infection in the mosquito. Activation of this pathway negatively regulates lipophorin expression, an important lipid transporter that both enhances egg development and Plasmodium infection. This is an unrecognized cross-phyla interaction between a mosquito and its vertebrate host. These processes are critical to understanding the complex life cycle of mosquitoes and Plasmodium following a blood meal and may be applicable to other hematophagous arthropods and vector-borne infectious agents.

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Section: Discussionmentioning

confidence: 99%

Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

Chuang,

Stone,

Abouneameh

et al. 2024

mBio

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“…Adiponectin (AdipoQ) and its receptor (AdipoR) are closely associated with skeletal muscle growth and development, along with glucose and lipid metabolism (Kim et al 2016). Decreased AdipoR expression results in diminished fatty acid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis provides insights into a molecular mechanism of histamine response in the cyprid larvae of Amphibalanus amphitrite

Jiang

Sun

Jin

et al. 2022

Mar. Ecol. Prog. Ser.

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Barnacles are notorious marine fouling creatures; their planktonic cyprid larvae attach to material substrates and metamorphose. Histamine has shown great importance in regulating cyprid settlement and metamorphosis. This study aimed to investigate the mechanisms of histamine-induced larval settlement. Cyprids were exposed to histamine or loratadine, an anti-histamine compound. The percent larval settlements of the histamine- and anti-histamine-treated cyprids were significantly higher and lower, respectively, than the control group. Transcriptomic analyses showed that histamine-treated cyprids had 18498 differentially expressed genes (DEGs, 14531 up-regulated, 3967 down-regulated) and the anti-histamine group had 18055 DEGs (17237 up-regulated, 818 down-regulated) in comparison to untreated controls. In both treatment groups, significant enrichment of DEGs involved in the mitogen-activated protein kinase signaling pathway was observed. Based on the results of larval settlement bioassays, we set 4 filter conditions to perform DEG analyses, and 19 DEGs were selected as functional genes related to cyprid settlement. The functional categories of these genes included structural proteins, spider silk proteins, energy metabolism proteins, cement proteins, glycosyl proteins, and multifunctional proteins. The energy metabolism protein AdipoR was significantly up-regulated in the histamine-treated cyprids but significantly down-regulated in the anti-histamine group. The activity of adenosine monophosphate-activated protein kinase, a downstream signaling protein of AdipoR, increased in the histamine-treated group and decreased in the anti-histamine-treated group. Our results provide new insights into the molecular mechanisms underlying the histamine-induced settlement of barnacle cyprids and identify AdipoR as an important gene that can affect the settlement of cyprids, likely through regulating cyprid energy metabolism.

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Identification, characterization, and expression analysis of adiponectin receptors in black carp Mylopharyngodon piceus in response to dietary carbohydrate

Gao

Chen

et al. 2018

Fish Physiol Biochem

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In the present study, three cDNAs of AdipoRs (MpAdipoR1a, MpAdipoR1b, and MpAdipoR2) were identified from juvenile black carp Mylopharyngodon piceus. There were 375, 378, and 356 amino acids in the MpAdipoR1a, MpAdipoR1b, and MpAdipoR2, respectively. BLAST analysis reveals that MpAdipoRs share high identities with other known AdipoRs from zebrafish, rainbow trout, human, etc. And there were all seven transmembrane regions in the amino acid sequences of MpAdipoR1s and MpAdipoR2, respectively. The relative expression levels of MpAdipoR1s were higher in the liver, blood, brain, and eyes in black carp (p < 0.05). Relatively higher expression of MpAdipoR2 was detected in the liver (p < 0.05) and then in the adipose tissues and blood by real-time PCR assays. The relative expression levels of AdipoR mRNA in the liver, muscle, brain, blood, and adipose tissues were detected by real-time PCR in black carp fed with four levels of dietary carbohydrate (CHO) (10.65, 19.43, 28.84, and 37.91%) for 9 weeks, respectively. The expression levels of MpAdipoR1s in the liver, muscle, brain, and blood were induced and reached to the maximum at optimal dietary CHO (19.43 or 28.84%) and then were decreased at 37.91% dietary CHO, although there were no significant differences on the expression levels of MpAdipoR1a in the liver between 19.43, 28.84, and 37.91% dietary CHO. Similarly, MpAdipoR2 were also induced to the maximal levels in the liver, muscle, brain, and blood at optimal dietary CHO (19.43 or 28.84%) compared with that at 10.65% dietary CHO, although no significant differences were observed on the expression levels of MpAdipoR2 in the liver and muscle between groups fed 19.43 and 37.91% dietary CHO (p > 0.05). However, the expression levels of MpAdipoRs in the adipose tissues were significantly downregulated at excessive dietary CHO (37.91%) compared with other groups (p < 0.05). These results indicated that MpAdipoRs were inducible proteins and might enhance the understanding of their vital roles in the regulation of glucose metabolic homeostasis in black carp.

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Molecular characterization of an adiponectin receptor homolog in the white leg shrimp,Litopenaeus vannamei

Cited by 8 publications

References 65 publications

Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

Transcriptome analysis provides insights into a molecular mechanism of histamine response in the cyprid larvae of Amphibalanus amphitrite

Identification, characterization, and expression analysis of adiponectin receptors in black carp Mylopharyngodon piceus in response to dietary carbohydrate

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