Potential probiotic and safety characterisation of enterococcus bacteria isolated from indigenous fermented motal cheese

Kouhi, Fardin; Mirzaei, Hamid; Nami, Yousef; Khandaghi, Jalil; Javadi, Afshin

doi:10.1016/j.idairyj.2021.105247

Cited by 31 publications

(22 citation statements)

References 62 publications

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“…The adhesion rates of these isolates to Caco-2 cells were between 23 and 38%. It seems that adhesion ability is correlated with auto-aggregation 46 . These results also support the correlation between adhesion and auto-aggregation.…”

Section: Discussionmentioning

confidence: 99%

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Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples

Mazlumi

Panahi

Hejazi

et al. 2022

Sci Rep

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This research aimed to isolate lactic acid bacteria from the bowel of saltwater fish to assess their potential probiotic properties. Nineteen isolates of LAB including Lactiplantibacillus plantarum, Lactiplantibacillus pentosus, Lactobacillus acidophilus, Levilactobacillus brevis, Pediococcus pentosaceus, and Pediococcus acidilactici were recognized using molecular tools. All the isolates survived in the simulated conditions of the GI tract. Auto-aggregation ranged from 01.3 ± 0.5 to 82.6 ± 1.4% and hydrophobicity with toluene ranged from 3.7 ± 1.6 to 69.4 ± 1.3%, while the range of hydrophobicity with xylene was from 02.2 ± 1.6 to 56.4 ± 2.1%. All the isolates of lactobacilli, pediococci, enterococci, and lactococci indicated variable sensitivity and resistance towards clinical antibiotics. Non-neutralized cell free supernatant of isolates F12 and F15 showed antimicrobial activity against all the 8 evaluated enteric pathogens. Cluster analysis of identified potential probiotic bacteria based on heat-map and PCA methods also highlighted the priority of isolates F3, F7, F12, and F15 as bio-control agents in fishery industry. The findings of this study may essentially contribute to the understanding of the probiotic potential of LAB in saltwater fish, in order to access their probiotic characterization for use as biocontrol in fishery.

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Section: Discussionmentioning

confidence: 99%

“…There are many studies, which investigated the cholesterol reduction ability of LAB isolates 28 , 46 , 47 . The reduction of serum cholesterol values is important to prevent the coronary heart disease due to the main risk factor for the progression of coronary heart disease is hypercholesterolemia 48 .…”

Section: Discussionmentioning

confidence: 99%

Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples

Mazlumi

Panahi

Hejazi

et al. 2022

Sci Rep

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“…Strains with survival rates of more than 80% were chosen for further study 21 . The growth potential of the isolate was tested in the presence of 0.3% w/v bile salts.…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity of potential probiotic Limosilactobacillus fermentum strains and their role against toxigenic aflatoxin-producing aspergilli

Mahjoory

Mohammadi

Hejazi

et al. 2023

Sci Rep

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Two major aflatoxin-producing strains are Aspergillus flavus and Aspergillus niger. Probiotic bacteria have been identified as a potential means to fight aspergilli and reduce the availability of aflatoxin (AFs) as well as other food contaminants. In this study, the potential of ABRIIFBI-6 and ABRIIFBI-7 strains to inhibit the growth of aspergilli was investigated. Both strains survived in the simulated gastrointestinal conditions and inhibited the growth of Aspergillus significantly. Auto-aggregation ranged from 67.4 ± 1.9 for ABRIIFBI-6 to 75.8 ± 2.3% for ABRIIFBI-7, and hydrophobicity ranged from 57.3 ± 1.6 to 61.2 ± 1.4% for ABRIIFBI-6 and ranged from 51.2 ± 1.4 to 55.4 ± 1.8% for ABRIIFBI-7. The ranges of coaggregation with Staphylococcus aureus were 51.3 ± 1.7 and 52.4 ± 1.8% for ABRIIFBI-6 and ABRIIFBI-7, respectively, while coaggregation with Bacillus cereus was 57.9 ± 2.1 and 49.3 ± 1.9% for ABRIIFBI-6 and ABRIIFBI-7, respectively. Both strains indicated remarkable sensitivity to clinical antibiotics. According to the analysis of the identified potential probiotics, the findings of this study could significantly contribute to the understanding of the probiotic potential of LAB in dairy products in order to access their probiotic characterization for use as biocontrol of aflatoxin-producing species.

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“…Although numerous strains of Bifidobacterium and Lactobacillus species have been reported to alleviate cholesterol both in vivo and in vitro , very limited reports are available for the hypocholesterolemic effect of the Enterococcus strains (Tomaro-Duchesneau et al, 2014 ; Shehata et al, 2016 ; Hyrslova et al, 2021 ; Marras et al, 2021 ; Yang et al, 2021 ; Kouhi et al, 2022 ). Thus, this study has aimed to determine the beneficial effects of probiotic Enterococcus durans HS03 (BSH-negative) and Enterococcus lactis YY1 (BSH-positive) on lipid metabolism in Sprague–Dawley rats fed with a high-cholesterol diet.…”

Section: Introductionmentioning

confidence: 99%

Revealing Probiotic Potential of Enterococcus Strains Isolated From Traditionally Fermented Chhurpi and Healthy Human Gut

Ghatani

Thapa

Sha³

et al. 2022

Front. Microbiol.

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In this study, the two lactic acid bacterial strains Enterococcus durans and Enterococcus lactis previously isolated from soft chhurpi, a traditionally fermented milk product prepared by the indigenous community of Sikkim Himalayas and healthy human gut were used. In this study, we attempted to investigate the probiotic attributes, safety, and health beneficial role, and hypercholesterolemia of Enterococcus durans and Enterococcus lactis. Both probiotic potential strains showed good hypocholesterolemic activity in vitro along with tolerance to acid pH (2 and 2.5), tolerance to three bile salts, oxbile, cholic acid, and taurocholic acid (0.5 and 1%), presence of BSH enzyme and its activity, and cell surface adherence. On assessing for safety, both LAB strains were sensitive to antibiotics and exhibited no hemolytic activity. The probiotic strains were tested in vivo in the Sprague–Dawley rats which were divided into five experimental groups: Normal Control (ND), probiotic strain Enterococcus durans HS03 (BSH-negative) and high-cholesterol diet (HCD1), probiotic strain Enterococcus lactis YY1 (BSH-positive) and high-cholesterol diet (HCD2), and a combination of both strains and high-cholesterol diet (HCD3) and Negative Control (HCD). The probiotic-treated groups HCD1, HCD2, and HCD3 showed a decrease in serum cholesterol levels up to 22.55, 6.67, and 31.06%; the TG and VLDL concentrations were 25.39, 26.3, and 33.21%; reduction in LDL-cholesterol was 33.66, 28.50, and 35.87%; and increase of HDL was 38.32, 47.9, and 41.92%. Similarly, the effects of total cholesterol and TG in the liver, kidney and liver histopathology, liver and body lipid index, and oxidative stress in rat liver were also studied. The fecal lactobacilli were more in the samples of the probiotic-treated groups and their fecal coliform and E. coli counts decreased relatively as compared to the control groups in 0, 7, 14, and 21 days. This is the first report on the probiotic potential of Enterococcus durans HS03 and Enterococcus lactis YY1 strains that gives a new insight into the cholesterol-lowering and probiotic product development with wide health attributes.

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Potential probiotic and safety characterisation of enterococcus bacteria isolated from indigenous fermented motal cheese

Cited by 31 publications

References 62 publications

Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples

Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples

Antifungal activity of potential probiotic Limosilactobacillus fermentum strains and their role against toxigenic aflatoxin-producing aspergilli

Revealing Probiotic Potential of Enterococcus Strains Isolated From Traditionally Fermented Chhurpi and Healthy Human Gut

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