Antifungal activity of potential probiotic Limosilactobacillus fermentum strains and their role against toxigenic aflatoxin-producing aspergilli

Mahjoory, Yalda; Mohammadi, Reza; Hejazi, Mohammad Amin; Nami, Yousef

doi:10.1038/s41598-023-27721-1

Cited by 19 publications

(12 citation statements)

References 37 publications

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“…Numerous studies have highlighted the potential of specific probiotic strains to exhibit anti-infective properties against intestinal microbes 7 – 9 . This anti-pathogenic activity against harmful bacteria underscores the importance of carefully considering probiotics in promoting health 16 . Probiotic strains designated as accepted probiotics are expected to demonstrate antimicrobial activities against both Gram-positive and Gram-negative pathogenic microorganisms.…”

Section: Resultsmentioning

confidence: 93%

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Anti-oral cancer properties of potential probiotic lactobacilli isolated from traditional milk, cheese, and yogurt

Nami,

Tavallaei,

Kiani

et al. 2024

Sci Rep

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This study investigates the probiotic and anti-cancer effects of 21 isolated Lactobacillus strains from cheese, milk, and yogurt in Kermanshah, Iran, on oral cancer cell lines KB and OSCC. Four selected isolates (Y33, M45, C5, and C28) displayed good viability and resistance to specific antibiotics. Notably, strains C28 and Y33 exhibited the best results, showing susceptibility or semi-susceptibility to five antibiotics. Y33, with high cell surface hydrophobicity (62%), demonstrated significant anti-pathogenic activity, inhibiting the growth of tested pathogens and displaying strong adhesion to human intestinal Caco-2 cells (52%). Further assessments, including acridine orange/ethidium bromide staining and mRNA expression analysis, revealed four isolates (C5, C28, M45, and Y33) with promising probiotic properties. Particularly, Y33’s protein-based extract metabolites showed dose- and time-dependent inhibition of KB and OSCC cancer cell lines, inducing apoptosis without significant cytotoxic effects on normal cells. Y33 (Lactiplantibacillus plantarum) exhibited the strongest probiotic potential, surpassing conventional anti-cancer drugs, suggesting its therapeutic potential for preventing oral cancer cell proliferation and improving survival rates in oral cancer patients.

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Section: Resultsmentioning

confidence: 93%

“…Auto-aggregation and co-aggregation are crucial mechanisms for probiotics in preventing surface colonization by pathogens 8 , 9 , 16 . The auto-aggregation assay revealed that isolate Y33 demonstrated the highest auto-aggregation percentage (57%).…”

Section: Resultsmentioning

confidence: 99%

Anti-oral cancer properties of potential probiotic lactobacilli isolated from traditional milk, cheese, and yogurt

Nami,

Tavallaei,

Kiani

et al. 2024

Sci Rep

Self Cite

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“…Natural resources have always provided solutions to produce several natural products such as antimicrobials(Hutchings et al 2019 ). Soil is a habitat for a diverse population of microbes with the potential for new metabolites that have not been discovered yet (Vehapi et al 2023 ; Mahjoory et al 2023 ). Some of these metabolites are produced as survival mechanisms against microbes present in their environments that we can utilize as antimicrobials.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic nanopore sequencing for exploring the nature of antimicrobial metabolites of Bacillus haynesii

Eltokhy,

Saad,

Eltayeb

et al. 2024

AMB Expr

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Multidrug-resistant (MDR) pathogens are a rising global health worry that imposes an urgent need for the discovery of novel antibiotics particularly those of natural origin. In this context, we aimed to use the metagenomic nanopore sequence analysis of soil microbiota coupled with the conventional phenotypic screening and genomic analysis for identifying the antimicrobial metabolites produced by promising soil isolate(s). In this study, whole metagenome analysis of the soil sample(s) was performed using MinION™ (Oxford Nanopore Technologies). Aligning and analysis of sequences for probable secondary metabolite gene clusters were extracted and analyzed using the antiSMASH version 2 and DeepBGC. Results of the metagenomic analysis showed the most abundant taxa were Bifidobacterium, Burkholderia, and Nocardiaceae (99.21%, followed by Sphingomonadaceae (82.03%) and B. haynesii (34%). Phenotypic screening of the respective soil samples has resulted in a promising Bacillus isolate that exhibited broad-spectrum antibacterial activities against various MDR pathogens. It was identified using microscopical, cultural, and molecular methods as Bacillus (B.) haynesii isolate MZ922052. The secondary metabolite gene analysis revealed the conservation of seven biosynthetic gene clusters of antibacterial metabolites namely, siderophore lichenicidin VK21-A1/A2 (95% identity), lichenysin (100%), fengycin (53%), terpenes (100%), bacteriocin (100%), Lasso peptide (95%) and bacillibactin (53%). In conclusion, metagenomic nanopore sequence analysis of soil samples coupled with conventional screening helped identify B. haynesii isolate MZ922052 harboring seven biosynthetic gene clusters of promising antimicrobial metabolites. This is the first report for identifying the bacteriocin, lichenysin, and fengycin biosynthetic gene clusters in B. haynesii MZ922052.

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“…Rare mechanisms present in only a few strains of a given species. Examples: those responsible for neurological (Dahiya & Nigam, 2023a), detoxification (Kariyawasam et al., 2021; Mahjoory et al., 2023), immunological and endocrine effects (Mändar et al., 2023), and the production of specific bioactive substances (Sun et al., 2023). …”

Section: Addition Of Dietary Supplements—diversification Of Functions...mentioning

confidence: 99%

From the past to the future: Fermented milks and their health effects against human diseases

Zhang,

Geng,

Cheng

et al. 2023

Food Frontiers

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The benefits of fermented milk have been known to mankind since the ancient days of civilization. The important role of fermented milk in human nutrition has been well documented through the ages, from the original unpredictable souring of milk caused by the microorganisms inherent in milk to modern microbiological processes, leading to fermented milk with the greater nutritional value produced under controlled conditions. The bioactive peptides synthesized by the microbial degradation of proteins in fermented milk and the addition of supplements, such as probiotics and prebiotics endow them with various physiological functions, including the regulation of the gut microbiota, cholesterol lowering, and immunomodulation, making them potential functional foods that are notable in healthy diets. Here, we summarized the development of fermented milk into four stages and also summarized their impact on health and diseases during different developmental stages. In addition, we described the characteristics and limitations of fermented milk in different eras. Accordingly, we integrated the future trends in the development of fermented milk as functional foods. It is hoped that the efforts to develop the potential of fermented milk as functional foods will help manage the health risks faced by many countries.

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Antifungal activity of potential probiotic Limosilactobacillus fermentum strains and their role against toxigenic aflatoxin-producing aspergilli

Cited by 19 publications

References 37 publications

Anti-oral cancer properties of potential probiotic lactobacilli isolated from traditional milk, cheese, and yogurt

Anti-oral cancer properties of potential probiotic lactobacilli isolated from traditional milk, cheese, and yogurt

Metagenomic nanopore sequencing for exploring the nature of antimicrobial metabolites of Bacillus haynesii

From the past to the future: Fermented milks and their health effects against human diseases

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