Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples

Abstract

This research aimed to isolate lactic acid bacteria from the bowel of saltwater fish to assess their potential probiotic properties. Nineteen isolates of LAB including Lactiplantibacillus plantarum, Lactiplantibacillus pentosus, Lactobacillus acidophilus, Levilactobacillus brevis, Pediococcus pentosaceus, and Pediococcus acidilactici were recognized using molecular tools. All the isolates survived in the simulated conditions of the GI tract. Auto-aggregation ranged from 01.3 ± 0.5 to 82.6 ± 1.4% and hydrophobicity with toluene ranged from 3.7 ± 1.6 to 69.4 ± 1.3%, while the range of hydrophobicity with xylene was from 02.2 ± 1.6 to 56.4 ± 2.1%. All the isolates of lactobacilli, pediococci, enterococci, and lactococci indicated variable sensitivity and resistance towards clinical antibiotics. Non-neutralized cell free supernatant of isolates F12 and F15 showed antimicrobial activity against all the 8 evaluated enteric pathogens. Cluster analysis of identified potential probiotic bacteria based on heat-map and PCA methods also highlighted the priority of isolates F3, F7, F12, and F15 as bio-control agents in fishery industry. The findings of this study may essentially contribute to the understanding of the probiotic potential of LAB in saltwater fish, in order to access their probiotic characterization for use as biocontrol in fishery.

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

“…In addition, it was shown that strains C3 and C18 had the highest hydrophobic properties, with rates of 65.30 ± 2.08 and 62.10 ± 1.33% with xylene and toluene, respectively. These results confirm the results of several previous works reported by Mazlumi et al 26 , Kiani et al 23 and Nami et al 27 .…”

Cytotoxic effect of potential probiotic Lactiplantibacillus plantarum KUMS-Y8 isolated from traditional dairy samples on the KB and OSCC human cancer cell lines

“…Probiotics must adhere to the intestinal mucosa and not be easily removed from the gut by smoky bowel movements in order to colonize in the gut. Our findings are consistent with previous research, which found that only a few Lactobacillus strains, such as Y8 and C14, could adhere well to Caco-2 cells [ 41 ].…”

Biofilm formation and auto-aggregation abilities of novel targeted aqua-probiotics

“…In addition, constitutive or stressinduced bacterial aggregation has been shown to play an important role in bacteria-host interactions [88][89]. The candidates for targeted probiotics with lactic acid origin, including Lactiplantibacillus plantarum, Lactiplantibacillus pentosus, Lactobacillus acidophilus, Levilactobacillus brevis, Pediococcus pentosaceus, and Pedio-coccus acidilactici were also isolated and characterized by Mazlumi and coauthors [90]. Auto-aggregations of these bacteria was in the range from 01.3 ± 0.5 to 82.6 ± 1.4% [90].…”