Potential Biomarkers in the Sera of Breast Cancer Patients from Bahawalpur, Pakistan

Abstract

Most of the approximately 90,000 cases of Breast Cancer (BC) documented annually in Pakistan are not diagnosed properly because of lack of suitable markers. We performed serum proteome expression profiling of BC and benign breast disease (BBD) patients with the aim to identify biomarkers that can be helpful for diagnosis and prognosis of the disease. Sera of patients were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis (PAGE). Differentially expressed proteins were subjected to identification through LC-MS/MS analysis. In majority of the BC cases some acute phase proteins (APP) and some complement system components (C3 and C8) containing fractions were up-regulated with the exception of transthyretin (TTR) which was predominantly (68.75%) down-regulated (n = 33/48) in the sera of these patients. Varying expression patterns were observed in BBD patients and healthy controls. These differentially expressed proteins have the potential to serve as diagnostic biomarkers for BC as well as benign breast diseases.

Proteomic investigation on bio-corona of Au, Ag and Fe nanoparticles for the discovery of triple negative breast cancer serum protein biomarkers

“…This result was observed after the analysis of the protein corona formed around AuNPs (10.02 ± 0.91 nm). A similar observation was recently reported, where it was found that transthyretin (TTHY) was predominantly (68.75%) down-regulated (n = 33/48) in the sera of breast cancer patients [57].…”

Proteomic Analysis of Urine to Identify Breast Cancer Biomarker Candidates Using a Label-Free LC-MS/MS Approach

“…Protein DYH8 was previously detected in serum of normal health subjects [ 65 ]. Although HBA was reported as a potential serum biomarker in ovarian [ 39 ] and colon cancer [ 38 ], only hemoglobin subunit β was reported to be elevated in the BC patients [ 19 ]. Notably, Desmoglein-1, Kallikrein-1, Keratin, type II cytoskeleton 2 epidermal (K22E) and Poliovirus receptor (PVR) were all proteins significantly down regulated in IBC ( S1 Table ).…”

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

“…In the present work, three complement system components implicated in the innate immune response were identified in the PC for the different BC subtypes: complement component C8 beta chain (C8B) for LA, complement C5 (C5) for HER2+ and complement C2 (C2) for TNBC (see Table 2 ). Previous studies also found deregulation of some of these complement system components in the sera of BC patients [ 25 , 26 ].…”