Proteomic investigation on bio-corona of Au, Ag and Fe nanoparticles for the discovery of triple negative breast cancer serum protein biomarkers

Chantada-Vázquez, María Del Pilar; López, Antonio Castro; Vence, María García; Vázquez‐Estévez, Sergio; Nebril, Benigno Acea; Calatayud, David G.; Jardiel, Teresa; Bravo, Susana B.; Núñez, Cristina

doi:10.1016/j.jprot.2019.103581

Cited by 42 publications

(65 citation statements)

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“…AuNPs with a size of 12.96 ± 0.72 nm were prepared by a chemical reduction method [ 12 , 13 , 14 ]. As Figure 2 shows, proteins presented in serum samples (×2) were chemically reduced with dithiothreitol (DTT) and alkylated with iodoacetamide (IAA) before their ex vivo incubation with AuNPs (12.96 ± 0.72 nm) to get the formation of the PCs [ 12 , 13 , 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…Colloidal AuNPs with a size of 12.96 ± 0.72 nm were prepared by chemical reduction method as per the protocol developed previously [ 12 , 13 , 14 ]. In short, sodium citrate tribasic solution (0.075% w / v ) was dissolved in 60 mL warm distilled water under constant magnetic stirring.…”

Section: Methodsmentioning

confidence: 99%

“…Resultant serum were transferred to sterile cryovials, frozen and stored at −80 °C until further use at Research Unit, Lucus Augusti University Hospital (HULA). The formation of the ex vivo PC was achieved following the steps shown in Figure 2 [ 12 , 13 , 14 ]. After that, unbound serum proteins from the surface of AuNPs were removed by centrifugation at 18,840× g for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…SWATH/MS experiments were carried out following the instrumental parameters described elsewhere [ 13 ]. Briefly, two biological replicates of LA, LB-, LB+, HER+, TNBC and HC samples were used to get extensive quantitative data by label-free SWATH-MS analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Disease-associated biomarkers comprise less than 1% of serum proteins. In this way, through the formation of the PC, nanoparticles could act as sorbent materials for the enrichment of low-abundance peptides/proteins presented in serum samples before the biomarker identification by mass spectrometry (MS) analysis [ 12 , 13 , 14 ]. Importantly, otherwise undetectable changes in protein concentration at an early stage of the disease (as breast cancer), after any treatment (chemotherapy, immunotherapy) or surgery could be detected analyzing the PC composition [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Chantada-Vázquez

López

García-Vence

et al. 2020

IJMS

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Breast cancer (BC) is a molecularly heterogeneous disease that encompasses five major molecular subtypes (luminal A (LA), luminal B HER2 negative (LB-), luminal B HER2 positive (LB+), HER2 positive (HER2+) and triple negative breast cancer (TNBC)). BC treatment mainly depends on the identification of the specific subtype. Despite the correct identification, therapies could fail in some patients. Thus, further insights into the genetic and molecular status of the different BC subtypes could be very useful to improve the response of BC patients to the range of available therapies. In this way, we used gold nanoparticles (AuNPs, 12.96 ± 0.72 nm) as a scavenging tool in combination with Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) to quantitatively analyze the serum proteome alterations in the different breast cancer intrinsic subtypes. The differentially regulated proteins specific of each subtype were further analyzed with the bioinformatic tools STRING and PANTHER to identify the major molecular function, biological processes, cellular origin, protein class and biological pathways altered due to the heterogeneity in proteome of the different BC subtypes. Importantly, a profile of blood coagulation proteins was identified in the serum of HER2-overexpressing BC patients.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Chantada-Vázquez

López

García-Vence

et al. 2020

IJMS

Self Cite

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Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

Battaglini

Gümüş

Ciofani

2021

Adv Materials Technologies

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investigating the use of nanomaterials within living organisms. When a nanoparticle (NP) encounters biological molecules such as proteins, lipids, nucleic acids, or polysaccharides, it tends to adsorb these molecules on its surface due to electrostatic, hydrophobic, or other forms of interactions between the molecules and the surface of the NP. [1][2][3][4] Depending on their abundance and affinity, proteins and other molecules may form a hard corona that consists of tightly bounded proteins on the surface, or a soft corona, which indicates a second layer of proteins that are loosely attached to the proteins of the hard corona. [4] The composition of the soft corona changes over time according to the environmental conditions, and so the biological identity of nanostructures is usually, but not always, determined by the hard corona. This can be both advantageous and disadvantageous to the application of NPs. Some studies have focused on preventing biomolecular corona (BC) formation, as this may change the size and surface properties, hinder modifications on the surface, and cause the rapid detection and clearance of NPs by the immune system. [5,6] However, BCs can be exploited or even altered, in order to be made beneficial for targeting purposes as they can enable specific cells to more easily recognize NPs and may lead to a reduction in their toxicity. [7,8] A wide range of techniques have been used in BC studies, such as dynamic light scattering (DLS), [9] sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry (MS)-based approaches, [10][11][12][13][14][15][16][17][18][19][20][21] UV/vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), [22] atomic force microscopy (AFM), [23] scanning electron microscopy (SEM), [24][25][26][27] transmission electronic microscopy (TEM), [23] and circular dichroism spectroscopy (CD). [28] Various information (size, surface properties, morphology, structure, etc.) about BCs can be obtained through these methods. Many techniques have been applied in BC studies, which can be divided into microscopybased approaches that use direct imaging of the sample, and indirect techniques. Microscopy techniques can provide information about corona composition and shape with a spatial resolution up to the dimension scale of single protein molecules, but they are limited by the requirement for staining or extensive sample preparation. Indirect techniques can provide extensive information about BC-nanostructure interactions and properties, but typically have a limited resolution and so cannot analyze the BC at the level of single nanostructures. Other emerging

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Protein corona: Dr. Jekyll and Mr. Hyde of nanomedicine

Fasoli

2020

Biotechnology and Applied Biochemistry

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Nanomedicine is an interdisciplinary field of research, comprising science, engineering, and medicine. Many are the clinical applications of nanomedicine, such as molecular imaging, medical diagnostics, targeted therapy, and image-guided surgery. Despite major advances during the past 20 years, many efforts must be done to understand the complex behavior of nanoparticles (NPs) under physiological conditions, the kinetic and thermodynamic principles, involved in the rational design of NP. Once administrated in physiological environment, NPs interact with biomolecules and they are surrounded by protein corona (PC) or biocorona. PC can trigger an immune response, affecting NPs toxicity and targeting capacity. This review aims to provide a detailed description of biocorona and of parameters that are able to control PC formation and composition. Indeed, the review provides an overview about the role of PC in the modulation of both cytotoxicity and immune response as well as in the control of targeting capacity.

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Proteomic investigation on bio-corona of Au, Ag and Fe nanoparticles for the discovery of triple negative breast cancer serum protein biomarkers

Cited by 42 publications

References 64 publications

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

Protein corona: Dr. Jekyll and Mr. Hyde of nanomedicine

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