Potential Antidiabetic Activities of Probiotic Strains, <i>L. acidophilus</i> and <i>L. bulgaricus</i> against Fructose-Fed Hyperglycemic Rats

Abstract

Background: Deregulation of the gut microbiota results in various pathological disorders such as diabetes, inflammation, cancer, dyslipidemia etc. Modulation of intestinal microbiota by probiotics may facilitate the management of a number of clinical conditions of diabetes. Methods: The present study was designed to investigate the effect of feeding low-fat probiotic yogurt containing L. acidophilus and L. bulgaricus on fructose-fed hyperglycemic rats. Yogurt containing L. acidophilus or L. bulgaricus (9.5 × 10 9 cfu/rat/day) alone or in combination of both strains was supplied orally for 8 weeks concurrently with 20% fructose solution. Fasting blood glucose (FBG), oral glucose tolerance test, glycosylated hemoglobin (HbA1c), lipid profiles from blood and histopathological study of liver tissues were analyzed to evaluate anti-diabetic effect. Statistical analysis was done by Graph Pad Prism software. Values at p < 0.05 were considered statistically significant. Results: Administration of L. acidophilus or L. bulgaricus alone or in combination of both to hyperglycemic rats decreased serum FBG, onset of glucose intolerance, HbA1c, total cholesterol, triglycerides, LDL and VLDL-cholesterol, increased HDL-cholesterol levels significantly and preserved antioxidant pool such as activities of superoxide dismutase, catalase etc. Probiotic administration also prevented/repaired the oxidative damage of liver tissues. Conclusion: In conclusion, administration of yogurt containing L. acidophilus or L. bulgaricus that balanced the intestinal microbiota can prevent or lower risks of type-2 diabetes and its related complications.

Study on Diabetic Rats Comparing the Effects of Sitagliptin and Probiotics on Serum Glucose

“…According to a slew of research, probiotics can significantly reduce the risk of developing type 2 diabetes. [26] Sohag MSU et al [27] found that treatment of Probiotics reduced FBG in diabetic rats, which is consistent with our findings. Streptozotocin-induced diabetic mice were fed Probiotics, and their anti-hyperlipidemic effects were examined by Aggarwal J et al [28].…”

Ameliorative Effect of Probiotic Strains, Lactobacillus Acidophilus and Lactobacillus Bulgaricus against Acetic Acid-Induced Inflammation in the Mouse Colon

“…Morphological, physiological and biochemical examination of probiotics suggested that the isolated strains from the commercial product 'Probio' were Lactobacillus (L) acidophilus (LAB-A) and L. bulgaricus (LAB-B) that all features consistent to previous reports [19,20]. This morphological characterization has already been published [21]. After preparation of yogurt by inoculating probiotic strain L. acidophilus or L. bulgaricus in low fat UHT liquid milk at 37°C for 48 hours, colonies were counted.…”

Physicochemical properties and sensory acceptability of sugar free dark chocolate formulations added with probiotics

“…The combination of sodium alginate and maltodextrin increases the functional properties for the protection of diverse microorganisms (Krasaekoopt et al, 2003). According to literature, Lactobacillus platarum and Lactobacillus acidophilus are adequate probiotic candidates to prevent and ameliorate metabolic syndrome diseases such as type 2 diabetes (Sohag et al, 2019;Stevenson et al, 2014;Andrade-Velasques et al, 2021). Although there are previous reports in literature evaluating the development of chocolates with prebiotic, probiotic and synbiotic characteristics (Konar et al, 2016), there is scares information on the design of a chocolate formulations for the diabetic population, which should be sugarfree and also provide prebiotic and probiotic characteristics.…”