Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Kencana, Gusti Ayu Yuniati; Suartha, I Nyoman; Kardena, I Made; Dewi, Gusti Ayu Mayani Kristina; Nurhandayani, Arini; Syamsidar,; Agustina, Kadek Karang

doi:10.14202/vetworld.2018.1637-1640

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…Compared with inactivated EDS vaccine, the fiber protein could induce an equal level of HI and neutralizing antibodies, indicating good immunogenicity of the fiber protein. Serologically, the fiber protein was able to produce significantly higher protective antibody titer than that induced by whole viral DAdV-1 in the second week after vaccination (24). In laying hens, both the fiber protein and inactivated EDS vaccine provided complete protection against DAdV-1 infection.…”

Section: Discussionmentioning

confidence: 94%

Fiber Protein Produced in Escherichia coli as a Subunit Vaccine Candidate Against Egg-Drop Syndrome 76

et al. 2022

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The egg-drop syndrome ‘76 (EDS ‘76) caused by duck atadenovirus A (DAdV-1) infection in laying hens leads to the decrease in egg production, causing heavy economic losses in the poultry industry; thus, vaccines with high safety and immunogenicity are needed. In this study, the DAdV-1 fiber protein expressed in Escherichia coli with codon optimization showed the hemagglutination (HA) titer of 13 log2 after purification (0.6 mg/mL). Compared with inactivated EDS ‘76 vaccine, the specific pathogen-free chickens immunized with 0.4 mL fiber protein (HA titer of 11 log2) induced an equal level of HA inhibition (HI) titer and neutralizing antibodies. Meanwhile, after immunization with fiber protein, the lowest HI titer that could provide the effect to reduce egg production rate in laying hens after the challenge was 7 log2. Moreover, fiber protein with an HA titer of 7 log2 could induce an HI titer no <7 log2 in laying hens, which was equal to or higher than the lowest HI titer (7 log2) that could reduce egg production against DAdV-1 infection significantly, indicating that it is economically feasible for vaccine development. Importantly, the HI antibodies maintained at a high level up to 180 days postimmunization contribute to the clinical application of the vaccine candidate. Overall, the fiber protein produced in E. coli is an effective subunit vaccine candidate in EDS ‘76 control for its high immunogenicity and protection in chickens.

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Section: Discussionmentioning

confidence: 94%

Fiber Protein Produced in Escherichia coli as a Subunit Vaccine Candidate Against Egg-Drop Syndrome 76

et al. 2022

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“…Peningkatan populasi ayam petelur belum diikuti dengan peningkatan produktivitasnya (Setiawati et al, 2016). Faktor penyakit merupakan salah satu penyebab utama terjadinya penurunan produksi telur (Kencana et al, 2018).…”

Section: Pendahuluanunclassified

Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali

Wardani

Kencana

Winaya

et al. 2022

bulvet

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Avian Influenza H5N1 termasuk ke dalam kelompok penyakit menular strategis dan bersifat zoonosis mematikan baik pada hewan maupun manusia yang terinfeksi. Salah satu strategi utama dalam pencegahan AI di Indonesia yaitu dengan vaksinasi. Efektivitas vaksin AI akan lebih baik apabila strain virus dalam vaksin yang digunakan homolog dengan strain virus yang ada di lapangan. Penelitian ini bertujuan untuk mengetahui histopatologi limpa ayam petelur pascavaksinasi AI-H5N1 isolat dari Bali dengan parameter diameter folikel limfoid pulpa putih limpa. Sebanyak 36 ekor ayam petelur strain Novogen Brown digunakan untuk sampel penelitian. Sampel dibagi menjadi 2 kelompok yaitu P0/kelompok kontrol tanpa perlakuan dan P1 kelompok dengan perlakuan vaksinasi AI-H5N1 isolat dari Bali. Masing-masing kelompok terdiri dari 18 ekor ayam petelur. Ayam dipelihara sejak berusia 1 hari. Vaksinasi dilakukan setelah ayam berumur 5 minggu dan di booster pada umur 10 minggu dengan dosis vaksin 0,5 ml/ekor. Pengambilan sampel organ limpa dilakukan tiga minggu pascavaksinasi booster untuk pembuatan preparat histopatologi. Data hasil pemeriksaan preparat histopatologi limpa ayam petelur dianalisis dengan uji-t. Hasil penelitian pengukuran pada organ limpa, menunjukkan rerata diameter folikel limfoid pulpa putih limpa ayam petelur dengan perlakuan vaksinasi AI-H5N1 isolat dari Bali memiliki diameter lebih besar dibandingkan dengan kelompok ayam yang tidak divaksinasi. Simpulan dari penelitian ini adalah vaksinasi AI-H5N1 isolat Bali berpengaruh nyata terhadap penambahan diameter folikel limfoid pulpa putih limpa ayam petelur pascavaksinasi.

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“…Kencana et al (2017) menjelaskan bahwa vaksin kombinasi inaktif ND-IB-EDS menunjukkan ayam petelur titer antibodi EDS yang tidak protektif, hanya protektif terhadap ND. Penelitian lain dari Kencana et al, (2018) menjelaskan bahwa kandidat vaksin untuk EDSV di Indonesia berasal dari isolate di Medan. Pengembangan vaksin tersebut terbukti aman pada uji klinis sebagai kandidat vaksin EDSV asli Indonesia dan mampu menstimulasi pembentukan antibodi 2 minggu setelah vaksin dengan titer antibodi 2 5 HI units dan 2 7.3 HI units untuk 3 minggu pasca vaksinasi.…”

Section: Pengembangan Vaksin Edsvunclassified

Review: Strategi Praktis Penanganan Egg Drop Syndrome pada Ayam Petelur

Khasanah¹,

Silaban²,

Priyono³

et al. 2021

Jurnal Sain Peternakan Indonesia

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Layer poultry business is a prospective business due to meeting the need for animal protein from eggs. The average consumption of eggs is 2,152 kg per capita per week. These promising business prospects were not accompanied by an increase in the productivity of laying hens. Abnormal conditions such as cases of poultry disease will plunge the supply of eggs into a drop. One disease that is considerably popular in laying birds is Egg Drop Syndrome (EDS). This review aims to examine the EDS handling strategies in laying birds to prevent decreased egg production. Materials and methods through literature study. Some strategies that farmers can take to prevent EDS are vaccination, maintaining sanitation and cleanliness of pond land, including biosecurity, and using herbs as feed additives.

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Potential and safety tests of egg drop syndrome candidate vaccine from Medan isolate, Indonesia

Cited by 7 publications

References 12 publications

Fiber Protein Produced in Escherichia coli as a Subunit Vaccine Candidate Against Egg-Drop Syndrome 76

Fiber Protein Produced in Escherichia coli as a Subunit Vaccine Candidate Against Egg-Drop Syndrome 76

Histopatologi Limpa Ayam Petelur Pascavaksinasi Avian Influenza H5N1 Isolat dari Bali

Review: Strategi Praktis Penanganan Egg Drop Syndrome pada Ayam Petelur

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